Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 71
Aptamer sequence: GGCGAGTAGGTTGGTGTGGTTGGGGCTCGCC
Target unique ID: P00734
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_71 D4-7 Protein THRB_HUMAN N/A Tris-HCl pH 8.0, 5 mM KCl, 100 mM NaCl 17016675
Structure information of aptamer
Aptamer Sequence: GGCGAGTAGGTTGGTGTGGTTGGGGCTCGCC
The optimal secondary structure in dot-bracket notation: (((((((.................)))))))
The centroid secondary structure in dot-bracket notation: (((((((.................)))))))
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_71 Description D4-7
Aptamer chemistry DNA Length 31 nt
GC content 67.7% Molecular weight 9,951.14 Da
Molarity of 1 μg/μl solution 100.49 μM Number of G-quadruplexes 2
G-Score 20 Function Targeted binding
Sequence GGCGAGTAGGTTGGTGTGGTTGGGGCTCGCC
Applications Thrombin inhibiting
Target information
Type Detail Type Detail
Uniprot ID P00734 Protein name Prothrombin (EC 3.4.21.5) (Coagulation factor II) [Cleaved into: Activation peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain]
Gene name(s) F2 Organism Prothrombin (EC 3.4.21.5) (Coagulation factor II) [Cleaved into: Activation peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain]
Status reviewed in uniprot database Length 622
Mass 70,037 Activity regulation Inhibited by SERPINA5
Involvement in disease Factor II deficiency (FA2D) Catalytic activity Reaction=Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B
PDB ID(s)
1A2C;1A3B;1A3E;1A46;1A4W;1A5G;1A61;1ABI;1ABJ;1AD8;1AE8;1AFE;1AHT;1AI8;1AIX;1AWF;1AWH;1AY6;1B5G;1B7X;1BA8;1BB0;1BCU;1BHX;1BMM;1BMN;1BTH;1C1U;1C1V;1C1W;1C4U;1C4V;1C4Y;1C5L;1C5N;1C5O;1CA8;1D3D;1D3P;1D3Q;1D3T;1D4P;1D6W;1D9I;1DE7;1DIT;1DM4;1DOJ;1DWB;1DWC;1DWD;1DWE;1DX5;1E0F;1EB1;1EOJ;1EOL;1FPC;1FPH;1G30;1G32;1G37;1GHV;1GHW;1GHX;1GHY;1GJ4;1GJ5;1H8D;1H8I;1HAG;1HAH;1HAI;1HAO;1HAP;1HBT;1HDT;1HGT;1HLT;1HUT;1HXE;1HXF;1IHS;1IHT;1JMO;1JOU;1JWT;1K21;1K22;1KTS;1KTT;1LHC;1LHD;1LHE;1LHF;1LHG;1MH0;1MU6;1MU8;1MUE;1NM6;1NO9;1NRN;1NRO;1NRP;1NRQ;1NRR;1NRS;1NT1;1NU7;1NU9;1NY2;1NZQ;1O0D;1O2G;1O5G;1OOK;1OYT;1P8V;1PPB;1QBV;1QHR;1QJ1;1QJ6;1QJ7;1QUR;1RD3;1RIW;1SB1;1SFQ;1SG8;1SGI;1SHH;1SL3;1SR5;1T4U;1T4V;1TA2;1TA6;1TB6;1TBZ;1THP;1THR;1THS;1TMB;1TMT;1TMU;1TOM;1TQ0;1TQ7;1TWX;1UMA;1UVS;1VR1;1VZQ;1W7G;1WAY;1WBG;1XM1;1XMN;1YPE;1YPG;1YPJ;1YPK;1YPL;1YPM;1Z71;1Z8I;1Z8J;1ZGI;1ZGV;1ZRB;2A0Q;2A2X;2A45;2AFQ;2ANK;2ANM;2B5T;2BDY;2BVR;2BVS;2BVX;2BXT;2BXU;2C8W;2C8X;2C8Y;2C8Z;2C90;2C93;2CF8;2CF9;2CN0;2FEQ;2FES;2GDE;2GP9;2H9T;2HGT;2HNT;2HPP;2HPQ;2HWL;2JH0;2JH5;2JH6;2OD3;2PGB;2PGQ;2PKS;2PW8;2R2M;2THF;2UUF;2UUJ;2UUK;2V3H;2V3O;2ZC9;2ZDA;2ZDV;2ZF0;2ZFF;2ZFP;2ZFQ;2ZFR;2ZG0;2ZGB;2ZGX;2ZHE;2ZHF;2ZHQ;2ZHW;2ZI2;2ZIQ;2ZNK;2ZO3;3B23;3B9F;3BEF;3BEI;3BF6;3BIU;3BIV;3BV9;3C1K;3C27;3D49;3DA9;3DD2;3DHK;3DT0;3DUX;3E6P;3EE0;3EGK;3EQ0;3F68;3GIC;3GIS;3HAT;3HKJ;3HTC;3JZ1;3JZ2;3K65;3LDX;3LU9;3NXP;3P17;3P6Z;3P70;3PMH;3PO1;3QDZ;3QGN;3QLP;3QTO;3QTV;3QWC;3QX5;3R3G;3RLW;3RLY;3RM0;3RM2;3RML;3RMM;3RMN;3RMO;3S7H;3S7K;3SHA;3SHC;3SI3;3SI4;3SQE;3SQH;3SV2;3T5F;3TU7;3U69;3U8O;3U8R;3U8T;3U98;3U9A;3UTU;3UWJ;3VXE;3VXF;4AX9;4AYV;4AYY;4AZ2;4BAH;4BAK;4BAM;4BAN;4BAO;4BAQ;4BOH;4CH2;4CH8;4DIH;4DII;4DT7;4DY7;4E05;4E06;4E7R;4H6S;4H6T;4HFP;4HTC;4HZH;4I7Y;4LOY;4LXB;4LZ1;4LZ4;4MLF;4NZQ;4O03;4RKJ;4RKO;4RN6;4THN;4UD9;4UDW;4UE7;4UEH;4UFD;4UFE;4UFF;4UFG;4YES;5A2M;5AF9;5AFY;5AFZ;5AHG;5CMX;5DO4;5E8E;5EDK;5EDM;5EW1;5EW2;5GDS;5GIM;5JDU;5JFD;5JZY;5L6N;5MJT;5MLS;5MM6;5NHU;5TO3;5Z5W;5Z5X;6BJR;6C2W;6EO6;6EO7;6EO8;6EO9;6V5T;7KME;8KME;
Function Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C.
Activity data

No relevant experimental diagram

 Interaction ID 71
Target type Protein
Target unique ID P00734
Activity N/A
Binding Conditions/Buffer

Tris-HCl pH 8.0, 5 mM KCl, 100 mM NaCl
Assay

N/A
PubMed ID 17016675
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_69 DNA AGCGAGTAGGTTGGTGTGGTTGGGGCTCGCT
93.55%
Apta_73 DNA GGGGAGTAGGTTGGTGTGGTTGGGGCTCCCC
93.55%
Apta_74 DNA GGACAGTAGGTTGGTGTGGTTGGGGCTGTCC
87.10%
Apta_72 DNA AGTCAGTAGGTTGGTGTGGTTGGGGCTGACT
80.65%
Apta_70 DNA AGGTCGTAGGTTGGTGTGGTTGGGGCGACCT 74.19%
Apta_75 DNA CACTGGTAGGTTGGTGTGGTTGGGGCCAGTG 74.19%
Apta_25 DNA TGTGGGGGTGGTTGGGCGGGGCTGC 64.52%
Apta_63 DNA GACCCGTGGTAGGGTAGGATGGGGTGGTC 64.52%
Apta_350 DNA GGTGGTGGTGGTTGTGGTGGTGGTGG 64.52%
Apta_1085 DNA TGGGGTGGTGGTGTGGGTGGGGGTTTGTTC 64.52%
Apta_1241 DNA GGTGTGGAGTGGTGTGTGTTGTTGTTTGTC 64.52%
Apta_1247 DNA GGTGGTTGCTGTTGTTTGTGGTTGGTGTGC 64.52%
Apta_646 DNA TGGAGCTTGGATTGATGTGGTGTGTGAGTGCGGTGCCC 63.16%
Apta_1210 DNA GGGGAGGCAGTGTGTTGTGTCGTGTGTGTGCTTGG 62.86%
Apta_1258 DNA GGCGAAGGAGTCATGTAGGTGTGGTCGAGACCGCTGTGCT 62.50%
Apta_17 DNA AGGGGGGGGGGTGGGGGTGGGTCCC 61.29%
Apta_18 DNA TGGGGGGTTGGACGGGCGGGGCTGC 61.29%
Apta_124 DNA GGTGGTGGGGGGGGTTGGTAGGGTGTCTTC 61.29%
Apta_757 DNA GGTGGTGGTGGTTGTGGTGGTGG 61.29%
Apta_1094 DNA GGAATCGTGGGTGGTAGGGTAGGGGATGCA 61.29%

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AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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