Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1176
Aptamer sequence: GGGAATGGATCCACATCTACGAATTCGGAATGAGTGCCCTGCAAGCGAGGGCTAGCTTCACTGCAGACTTGACGAAGCTT
Target unique ID: P83673
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_1120 clone 6 Protein LYS1_CRAVI 128 nM 20 mM Tris pH 7.6, 100 mM NaCl, 5 mM MgCl2 15253644
Structure information of aptamer
Aptamer Sequence: GGGAATGGATCCACATCTACGAATTCGGAATGAGTGCCCTGCAAGCGAGGGCTAGCTTCACTGCAGACTTGACGAAGCTT
The optimal secondary structure in dot-bracket notation: .(((.....)))..........((((.....))))(((((.......))))).((((((..............)))))).
The centroid secondary structure in dot-bracket notation: ...................................(((((.......))))).((((((..............)))))).
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_1120 Description clone 6
Aptamer chemistry DNA Length 80 nt
GC content 52.5% Molecular weight 24,752.86 Da
Molarity of 1 μg/μl solution 40.40 μM Number of G-quadruplexes No QGRS found
G-Score N/A Function Target detection
Sequence GGGAATGGATCCACATCTACGAATTCGGAATGAGTGCCCTGCAAGCGAGGGCTAGCTTCACTGCAGACTTGACGAAGCTT
Applications Detection and Quantitation of Proteins
Target information
Type Detail Type Detail
Uniprot ID P83673 Protein name Lysozyme 1 (EC 3.2.1.17) (1,4-beta-N-acetylmuramidase 1) (Invertebrate-type lysozyme 1) (cv-lysozyme 1)
Gene name(s) lysoz1 lysoz Organism Lysozyme 1 (EC 3.2.1.17) (1,4-beta-N-acetylmuramidase 1) (Invertebrate-type lysozyme 1) (cv-lysozyme 1)
Status reviewed in uniprot database Length 184
Mass 19,986 Activity regulation N/A
Involvement in disease N/A Catalytic activity Reaction=Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
PDB ID(s)
N/A
Function Has antibacterial activity against the Gram-positive bacteria L.garvieae, M.luteus and Enterococcus sp.
Activity data
Interaction ID 1176
Target type Protein
Target unique ID P83673
Activity 128 nM
Binding Conditions/Buffer

20 mM Tris pH 7.6, 100 mM NaCl, 5 mM MgCl2
Assay

Fluorescence data showing the reactivity of the three anti-lysozyme aptamer clones after 10 min of washing
PubMed ID 15253644
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_1119 DNA GGGAATGGATCCACATCTACGAATTCGGTTGTGAAGATTGGGAGCGTCGTGGCTACTTCACTGCAGACTTGACGAAGCTT
82.50%
Apta_495 DNA GGGAATGGATCCACATCTACGTATTACTGCTGGTGACGAGGGCTAGACGACGTACCTTCACTGCAGACTTGACGAAGCTT 78.75%
Apta_1118 DNA GGGAATGGATCCACATCTACGAATTCATCAGGGCTAAAGAGTGCAGAGTTACTTAGTTCACTGCAGACTTGACGAAGCTT 77.50%
Apta_587 DNA GGAATGGATCCACATCCATGGATGGGCAATGCGGGGTGGAGAATGGTTGCCGCACTTCGGCTTCACTGCAGACTTGACGAAGCTT 69.41%
Apta_623 RNA GGGAAUGGAUCCACAUCUACGAAUUCACCGCGGGGUUGCGGACCGGGAGCUCCAGCUUCACUGCAGACUUGACGAAGCUU 61.25%
Apta_624 RNA GGGAAUGGAUCCACAUCUACGAAUUCGAAUUGCCGUAAUUUCCCGUGGAGCGAUGCUUCACUGCAGACUUGACGAAGCUU 61.25%
Apta_719 RNA GGAAUGGAUCCACAUCUACGAAGGCUUUGAAGGUGAGACCGUGCAAAUGAGGAUGGUGUGGAUGAUUAGGGUUGUCGGUUUUCACUGCAGACUUGACGAAGCUU 53.85%
Apta_589 DNA GGGAATTCGAGCTCGGTACCATCTGTGTAAGGGGTAAGGGGTGGGGGTGGGTACGTCTAGCTGCAGGCATGCAAGCTTGG 53.75%
Apta_1035 DNA ATGCGGATCCCGCGCGGGTCTGAGGAGTGCGCGGTGCCAGTGAGTGCGCGAAGCTTGCGC 53.75%
Apta_1101 DNA CCGTAATACGACTCACTATAGGTATGTGACGCGCGTCAATCGCTGTCCTACCAAGCTTTGCAGAGAGGATCCTT 53.75%
Apta_589 DNA GGGAATTCGAGCTCGGTACCATCTGTGTAAGGGGTAAGGGGTGGGGGTGGGTACGTCTAGCTGCAGGCATGCAAGCTTGG 53.75%
Apta_355 DNA GATGCAGCCACCTACATTGCAGGTTTTATAATACAACTACGCGCCGTTCGCAGGCTAGTT 52.50%
Apta_368 DNA GGTATTGAGGGTCGCATCTCAAAAGGGGTGATTGCTTGCACAATGACAGGGTAGGACAGATGGCTCTAACTCTCCTCT 52.50%
Apta_527 DNA ATACGGGAGCCAACACCAACAACCTAGATGTTCTGATAACACGAATCGCTTCGAAGAGCAGGTGTGACGGAT 52.50%
Apta_567 DNA CCTAAGGGGGGACTCAGCATTTTGTGCGGGCGGCGCTAACACAATCAGATAGAGCGGGGTT 52.50%
Apta_897 DNA ATACGGGAGCCAACACCACTCTTATTTCCGGAAGTGGTTCTTCAACAGAGGAATAGAGCAGGTGTGACGGAT 52.50%
Apta_898 DNA ATACGGGAGCCAACACCAACAAAACTAGCAAATTAGAGCCGTATCGGAGCCTATAGAGCAGGTGTGACGGAT 52.50%
Apta_935 DNA GACGCTTACTCAGGTGTGACTCGTCCGTCGGTTCGCCTTGCGAGTGCACGCCGCCAGCCGTTACATAAAGTCTGCCCACTCGAAGGACGCAGATGAAGTCTC 51.96%
Apta_68 DNA AGATGCCTGTCGAGCATGCTGTGAATAGGTAGGGTCGGATGGGCTACGGTGTAGCTAAACTGCTTTGTCGACGGG 51.25%
Apta_528 DNA ATACGGGAGCCAACACCACTTGGTTAACCAATCAAACCGGACAACGAAGGGTCCAGAGCAGGTGTGACGGAT 51.25%

ABOUT

AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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