Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 407
Aptamer sequence: ATCCAGAGTGACGCAGCAGATAAGTAGGTATCCGTTTGAAAAACTTTTCTGACCGTCCGACTATGGACACGGTGGCTTAGT
Target unique ID: P47871
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_401 | GR-3 | Protein | GLR_HUMAN | 52.7 nM | 5mM MgCl2 and 4.5 g/L glucose in Dulbecco's Phosphate Bufered Saline (D-PBS, pH=7.4), and 0.1mg/mL yeast tRNA and 1mg/L bovine serum albumin (BSA) | 28775305 |
Structure information of aptamer
| Aptamer Sequence: | ATCCAGAGTGACGCAGCAGATAAGTAGGTATCCGTTTGAAAAACTTTTCTGACCGTCCGACTATGGACACGGTGGCTTAGT |
| The optimal secondary structure in dot-bracket notation: | .((((.(((((((...((((.((((...((......))....)))).))))..))))..))).)))).............. |
| The centroid secondary structure in dot-bracket notation: | ................((((.((((.................)))).)))).....((......))............... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_401 | Description | GR-3 |
| Aptamer chemistry | DNA | Length | 81 nt |
| GC content | 46.9% | Molecular weight | 25,046.06 Da |
| Molarity of 1 μg/μl solution | 39.93 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted binding |
| Sequence | ATCCAGAGTGACGCAGCAGATAAGTAGGTATCCGTTTGAAAAACTTTTCTGACCGTCCGACTATGGACACGGTGGCTTAGT | ||
| Applications | Aptamer GR-3 against GCGR can be a promising tool with the potential to attenuate hyperglycemia in diabetes mellitus | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P47871 | Protein name | Glucagon receptor (GL-R) |
| Gene name(s) | GCGR | Organism | Glucagon receptor (GL-R) |
| Status | reviewed in uniprot database | Length | 477 |
| Mass | 54,009 | Activity regulation | N/A |
| Involvement in disease | Mahvash disease (MVAH) | Catalytic activity | N/A |
| PDB ID(s) | 2A83;3CZF;4ERS;4L6R;4LF3;5EE7;5XEZ;6LMK;6LML;6WHC;6WPW;7V35; |
 |
Function | G-protein coupled receptor for glucagon that plays a central role in the regulation of blood glucose levels and glucose homeostasis. |
Activity data
 |
Interaction ID | 407 |
| Target type | Protein | |
| Target unique ID | P47871 | |
| Activity | 52.7 nM | |
| Binding Conditions/Buffer | 5mM MgCl2 and 4.5 g/L glucose in Dulbecco's Phosphate Bufered Saline (D-PBS, pH=7.4), and 0.1mg/mL yeast tRNA and 1mg/L bovine serum albumin (BSA) |
|
| Assay | Dissociation constant (Kd) of GR-3 for CHO-GCGR cells was measured by fow cytometry |
|
| PubMed ID | 28775305 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_922 | DNA | ATCCAGAGTGACGCAGCACACCGTTTGTATTCTGCATTGTTTTGCATTCTACATGGACACGGTGGCTTAGT | |
| Apta_1089 | DNA | ATCCAGAGTGACGCAGCAATTACCGATGGCTTGTTTGTAATGTAGGGTTCCGTCGGATTGGACACGGTGGCTTAGT | |
| Apta_1088 | DNA | ATCCAGAGTGACGCAGCATCGAGTGGCTTGTTTGTAATGTAGGGTTCCGGTCGTGGGTTGGACACGGTGGCTTAGT | |
| Apta_786 | DNA | ATCCAGAGTGACGCAGCATTTGTATCCTATTTTGTTTATGTAATTGTCGTTGATCATGTGGACACGGTGGCTTAGT | |
| Apta_787 | DNA | ATCCAGAGTGACGCAGCATTTGTTTAATATGTTTTTTAATTCCCCTTGTGGTGTGTTGTGGACACGGTGGCTTAGT | |
| Apta_1090 | DNA | ATCCAGAGTGACGCAGCAGGGCTTGGGTTGGGAATAAGGATGTGGGAGGCGGCGAACATGGACACGGTGGCTTAGT | |
| Apta_785 | DNA | ATCCAGAGTGACGCAGCATGAATCTTTTCTTTTGGTTTTGATATTTATAGTTGGTGAATGGACACGGTGGCTTAGT | |
| Apta_794 | DNA | ATCCAGAGTGACGCAGCAAGGAGATTCGAGGGGGAAGGTTTGTTATAGGGGTTAATGGACACGGTGGCTTAGT | |
| Apta_1293 | DNA | ATCCAGAGTGACGCAGCATGCCCTAGTTACTACTACTCTTTTTAGCAAACGCCCTCGCTTTGGACACGGTGGCTTAGT | |
| Apta_725 | DNA | ATCCAGAGTGACGCAGCATGCTCATCCACGATTCTGGCGAATTTAGTGCCTGTCTTTTTCTCTTGGACACGGTGGCTTAGT | |
| Apta_921 | DNA | ATCCAGAGTGACGCAGCACGAGCCAGACATCTCACACCTGTTGCATATACATTTTGCATGGACACGGTGGCTTAGT | |
| Apta_923 | DNA | ATCCAGAGTGACGCAGCATCGACCTCTGTGCGAGCGGGTTGCATATATATTTTGCATGGACACGGTGGCTTAGT | |
| Apta_784 | DNA | ATCCAGAGTGACGCAGCATTTTGACGCTTTATCCTTTTCTTATGGCGGGATAGTTTCGTGGACACGGTGGCTTAGT | |
| Apta_920 | DNA | ATCCAGAGTGACGCAGCACGGCACTCACTCTTTGTTAAGTGGTCTGCTTCTTAACCTTCATCGACACGGTGGCTTA | |
| Apta_288 | DNA | ATCCAGAGTGACGCAGCATTTCATCGGGTCCAAAAGGGGCTGCTCGGGATTGCGGATATGGACACGT | |
| Apta_761 | DNA | AGCAGAGTTCACGACCCGATAAGGGGAAAACACATTTCGGATCTCCGTTGTCGCCCTGATACCGAGTTACATACCAATCGTCGCAG | |
| Apta_763 | DNA | AGCAGAGTTCACGACCCGATAAGGGGAAAACACATTTCGGATCTCCGTTGTCGCCCTGGTACCGAGTTACATACCAATCGTCGCAG | |
| Apta_764 | DNA | AGCAGAGTTCACGACCCGATAAGGGGAAAATACATTTCGGATCTCCGTTGTCGCCCTGATACCGAGTTACATACCAATCGTCGCAG | |
| Apta_765 | DNA | AGCAGAGTTCACGACCCGATAAGGGGAAAATACATTTCGGATCTCCGTTGTCGCCCTGGTACCGAGTTACATACCAATCGTCGCAG | |
| Apta_527 | DNA | ATACGGGAGCCAACACCAACAACCTAGATGTTCTGATAACACGAATCGCTTCGAAGAGCAGGTGTGACGGAT |
DEV TOOL
 |
 |
 |
 |