Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 923
Aptamer sequence: TGAGCCCAAGCCCTGGTATGTGATCGGTGACGAGGGTGCGGGGCGGGGGGTGAGGCACAGGGCAGGTCTACTTTGGGATC
Target unique ID: BAA-2587-PACK
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_837 Apt-2 Cell Enterohemorrhagic Escherichia coli O157:H7 (E. coli O157:H7) 20.73 nM 1× BB, 50 mmol/L Tris-HCl (pH 7.4), 5 mmol/L KCl, 100 mmol/L NaCl, and 1 mmol/L MgCl2 29756774
Structure information of aptamer
Aptamer Sequence: TGAGCCCAAGCCCTGGTATGTGATCGGTGACGAGGGTGCGGGGCGGGGGGTGAGGCACAGGGCAGGTCTACTTTGGGATC
The optimal secondary structure in dot-bracket notation: ....(((..((((((.(((....(((....)))..)))))))))..))).....((.....)).(((((......)))))
The centroid secondary structure in dot-bracket notation: ....(((..(((((.((((....(((....)))..)))))))))..)))...............(((((......)))))
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_837 Description Apt-2
Aptamer chemistry DNA Length 80 nt
GC content 65.0% Molecular weight 25,050.95 Da
Molarity of 1 μg/μl solution 39.92 μM Number of G-quadruplexes 3
G-Score 62 Function Targeted binding
Sequence TGAGCCCAAGCCCTGGTATGTGATCGGTGACGAGGGTGCGGGGCGGGGGGTGAGGCACAGGGCAGGTCTACTTTGGGATC
Applications Binding to Enterohemorrhagic Escherichia coli O157:H7 (E. coli O157:H7)
Target information
Type Detail Type Detail
ATCC ID BAA-2587-PACK Cell name Enterohemorrhagic Escherichia coli O157:H7 (E. coli O157:H7)
Category Bacteria Organism N/A
Product type Reporter-labeled bacterium Cell type N/A
Morphology N/A Tissue N/A
Disease N/A Product format Frozen
Antigen expression N/A Genes expressed N/A
Cell description A kit containing reporter-labeled Escherichia coli designed to distinguish control strain cross-contamination from true contamination in test samples. This product is appropriate for use as a positive control in quality control assays for E. coli.
Application(s) Assay development; Food testing; High-throughput screening
Activity data

No relevant experimental diagram

 Interaction ID 923
Target type Cell
Target unique ID BAA-2587-PACK
Activity 20.73 nM
Binding Conditions/Buffer

1× BB, 50 mmol/L Tris-HCl (pH 7.4), 5 mmol/L KCl, 100 mmol/L NaCl, and 1 mmol/L MgCl2
Assay

N/A
PubMed ID 29756774
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_838 DNA TGAGCCCAAGCCCTGGTATGTAGTAATGGTGCGTACAGGCGACGGGGTCCAGGCTGGAGGGGCAGGTCTACTTTGGGATC 75.00%
Apta_840 DNA TGAGCCCAAGCCCTGGTATGCGGATAACGAGGTATTCACGACTGGTCGTCAGGTATGGTTGGCAGGTCTACTTTGGGATC 73.75%
Apta_841 DNA TGAGCCCAAGCCCTGGTATGGTGTGCCTGTCGTTGTATTGGTCGGTAGGGATCGGAGTGGGGCAGGTCTACTTTGGGATC 72.50%
Apta_836 DNA TGAGCCCAAGCCCTGGTATGTTACAGTATGCTACCTCTACTTGAAGGTTGGTCGACGCGGGGCAGGTCTACTTTGGGATC 71.25%
Apta_844 DNA TGAGCCCAAGCCCTGGTATGTTGCGGGAGTCTAGCGGGCCACACTTTTATAGGTTCGCAGGGCAGGTCTACTTTGGGATC 71.25%
Apta_842 DNA TGAGCCCAAGCCCTGGTATGTGTGGGGTCCTGGATTATGTTTAGCGTCTTTCGCAGTGGGGGCAGGTCTACTTTGGGATC 70.00%
Apta_843 DNA TGAGCCCAAGCCCTGGTATGTCACATCCGGGTTCTTTGCGAACGCGTTTCCGCAGTCTCAGGCAGGTCTACTTTGGGATC 70.00%
Apta_839 DNA TGAGCCCAAGCCCTGGTATGAGCCCACGGAACACTGGTCGCGCCCACTGGTTTCTATATTGGCAGGTCTACTTTGGGATC 68.75%
Apta_568 DNA CCCAGCAGCACTGGTAGTGAGGCAGTTCACCGGTGGGGCGGTGAGTTTGGCTGCTATTTAT 55.00%
Apta_953 DNA TCACCTCATACGACTCACTATAGCGGATCCGAGCCGGGGTGTTCTGTTGGCGGGGGCGGTGGGCGGGCTGGCTCGAACAAGCTTGC 54.65%
Apta_151 DNA GCAATGGTACGGTACTTCCGCGGTCCGGGGTGGGTGGGTGGTGGGGGGTGCGGGGGGGCGGCCGCAAAAGTGCACGCTACTTTGCTAA 53.41%
Apta_589 DNA GGGAATTCGAGCTCGGTACCATCTGTGTAAGGGGTAAGGGGTGGGGGTGGGTACGTCTAGCTGCAGGCATGCAAGCTTGG 52.50%
Apta_616 DNA GGTGCGGGTAGGGATGAGGGCGGGTCGGGTTGAGTGTGAGCTTAGGGGtGGCGGATCTAC 52.50%
Apta_759 DNA GAGGCATACCAGCTTATTATTGGGGCCGGGGCAAGGGGGGGGTACCGTGGTAGGACAGATAGTAAGTGCAATCTGCGAA 52.50%
Apta_832 DNA CCGGAGGTGGGTGAGGTCTGCGGCAGGCTGTGTGGGTGGACCGGAGGGGGGTGAGGTCTGCGGCAGGCTGTGTGGGTGGA 52.50%
Apta_1011 DNA GCTGTGTGACTCCTGCAATGGTAGCGGGTAGGGGAGGGAGGGTGAATGGAGGATGTTCATGGCAGCTGTATCTTGTCTCC 52.50%
Apta_589 DNA GGGAATTCGAGCTCGGTACCATCTGTGTAAGGGGTAAGGGGTGGGGGTGGGTACGTCTAGCTGCAGGCATGCAAGCTTGG 52.50%
Apta_1187 DNA TGTACCGTCTGAGCGATTCGTACTTTATTCGGGAAGGGTATCAGCGGGGTTCAACAAGCCAGTCAGTGTTAAGGAGTGC 52.50%
Apta_812 DNA ATAGGAGTCACGACGACCAGAATAGAGATATGACAGCGGGGAAGGTTAAGAGGCGCTAGGAGTATGTGCGTCTACCTCTTGACTAAT 51.72%
Apta_76 DNA CAGCGCTAGGGCTTTTAGCGTAATGGGTAGGGTGGTGCGGTGCAGATATCGGAATTGGTG 51.25%

ABOUT

AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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