AptaDB: Result

Interaction ID: 921

Aptamer sequence: TATGGCGTGGCAAGCTTGGCCCGCTTCTCAAGCATGGTTATCTAC

Target unique ID: 25922

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_835	20-10	Cell	Escherichia Coli (E.Coli)	10.1 nM	1X PBS, 0.1 mg/mL salmon sperm, 1% bovine serum albumin, and 0.05% Tween-20	31837967

Aptamer Sequence:	TATGGCGTGGCAAGCTTGGCCCGCTTCTCAAGCATGGTTATCTAC
The optimal secondary structure in dot-bracket notation:	...((((.(((.......)))))))....................
The centroid secondary structure in dot-bracket notation:	....(((.(((.......)))))).....................

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_835	Description	20-10
Aptamer chemistry	DNA	Length	45 nt
GC content	53.3%	Molecular weight	13,818.86 Da
Molarity of 1 μg/μl solution	72.36 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted binding
Sequence	TATGGCGTGGCAAGCTTGGCCCGCTTCTCAAGCATGGTTATCTAC
Applications	Isolation of bacteria

Type	Detail	Type	Detail
ATCC ID	25922	Cell name	Escherichia Coli (E.Coli)
Category	Bacteria	Organism	N/A
Product type	Preceptrol culture	Cell type	N/A
Morphology	N/A	Tissue	N/A
Disease	N/A	Product format	Freeze-dried
Antigen expression	N/A	Genes expressed	N/A
Cell description	This strain does not produce verotoxin. This organism is a CLSI control strain for antimicrobial susceptibility testing. Used in susceptibility disc testing of neomycin, colistin [colimycin], kanamycin, cephalexin, gentamicins, cefamandole, cephalothin, tetracycline, cephaloglycin, cephaloridine [cephalomycin], nalidixic acid, and chloramphenicol.
Application(s)	Bioinformatics; Food testing; Media testing; Quality control

	Interaction ID	921
	Target type	Cell
	Target unique ID	25922
	Activity	10.1 nM
	Binding Conditions/Buffer	1X PBS, 0.1 mg/mL salmon sperm, 1% bovine serum albumin, and 0.05% Tween-20
	Assay	Binding saturation curve of aptamers
	PubMed ID	31837967

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_692	DNA	ACGTTGACGCTGGTGCCCGCTTGTGGTGCGAGTGTTGTGT	55.56%
Apta_722	DNA	CTCTCGGGACGACGGCCCGATCTCAGAGTAGTCGTCCC	55.56%
Apta_591	DNA	CTCTCGGGACGACGGCTGGCACGTTTGGTTCAAGAATGTGGGTGTCGTCCC	54.90%
Apta_35	DNA	TGGGTGGCCTCCCGTTTATTCGAGATTTGGTTGAATTTGT	53.33%
Apta_36	DNA	GTTAATGGGGGATCTCGCGGCCGTTCTTGTTGCTTATACA	53.33%
Apta_609	DNA	GCTGCGAGGTGGGTGGGTGGGAGCAATTGGTCCTCGCTTAGCTTCTACGGTGGGCTATCT	53.33%
Apta_610	DNA	GCTGTGAGGTGGGTGGGTGGGAGCAATTGGTCCTCGCTTAGCTTCTACGGTGGGCTATCT	53.33%
Apta_817	DNA	TGCGTGAGCGGTAGGCCCCTACGACCCACTGTGGTTGGGC	53.33%
Apta_912	DNA	TAGTATGGACTTGTCTTCTTGTCAGAGTCTGGCAGCACGT	53.33%
Apta_916	DNA	GGCGTATGCAGTTTTGTAGGGTTATCAAGGCTTGACGATT	53.33%
Apta_963	DNA	TGGCGCTGCCCCTAGTCTCCGGCTGATAGCTGCTTCTTGG	53.33%
Apta_309	DNA	TAGCCAAGGTAACCAGTACAAGGTGCTAAACGTAATGGCTTCGGCTTAC	53.06%
Apta_309	DNA	TAGCCAAGGTAACCAGTACAAGGTGCTAAACGTAATGGCTTCGGCTTAC	53.06%
Apta_974	DNA	CATGGTGTCAATACTATACCTTTTGCCGATGGTAACAGTTGGTGGTATCC	52.00%
Apta_1070	DNA	CCGTATCGCCCAGGCAACTGGGCTAAACTTCCCAGAGGGAACGAAACCTGGG	51.92%
Apta_573	DNA	CGTACAGTGGGCTATATTGCTGACGTAAGAGCTGCTTTCAATGCATTGGCATAGATGTCA	51.67%
Apta_611	DNA	GCTGCGAGGTGGGTGGGTGGGAGCAATTGGTCCTTGCTTAGCTTCTACGGTGGGCTATCT	51.67%
Apta_883	DNA	CGTACCGGCCAGTGATTACGACGAGACGAGCTTATGCGTATTGATGCCTAACTATCTACA	51.67%
Apta_993	DNA	AATCCGACCACGAGAGCAGACCGACCACAGCATTATCTAAACCCATTAGTCTAGTCCTGG	51.67%
Apta_1283	DNA	TACTGATGCAGGGTAATGCTTCTGTGTTTGTTCTTCATCATTAACGGCCGCGTCTGGCGG	51.67%

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