AptaDB: Result

Interaction ID: 920

Aptamer sequence: CGCAATACCAAAGTGGCGAGAGCGCTGTCTTGAGTGAGTGGTTGG

Target unique ID: 25922

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_834	20-7	Cell	Escherichia Coli (E.Coli)	8.0 nM	1X PBS, 0.1 mg/mL salmon sperm, 1% bovine serum albumin, and 0.05% Tween-20	31837967

Aptamer Sequence:	CGCAATACCAAAGTGGCGAGAGCGCTGTCTTGAGTGAGTGGTTGG
The optimal secondary structure in dot-bracket notation:	(((..(((..((((((((....))))).)))..))).))).....
The centroid secondary structure in dot-bracket notation:	(((..(((..((((((((....))))).)))..))).))).....

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_834	Description	20-7
Aptamer chemistry	DNA	Length	45 nt
GC content	55.6%	Molecular weight	14,021.99 Da
Molarity of 1 μg/μl solution	71.32 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted binding
Sequence	CGCAATACCAAAGTGGCGAGAGCGCTGTCTTGAGTGAGTGGTTGG
Applications	Isolation of bacteria

Type	Detail	Type	Detail
ATCC ID	25922	Cell name	Escherichia Coli (E.Coli)
Category	Bacteria	Organism	N/A
Product type	Preceptrol culture	Cell type	N/A
Morphology	N/A	Tissue	N/A
Disease	N/A	Product format	Freeze-dried
Antigen expression	N/A	Genes expressed	N/A
Cell description	This strain does not produce verotoxin. This organism is a CLSI control strain for antimicrobial susceptibility testing. Used in susceptibility disc testing of neomycin, colistin [colimycin], kanamycin, cephalexin, gentamicins, cefamandole, cephalothin, tetracycline, cephaloglycin, cephaloridine [cephalomycin], nalidixic acid, and chloramphenicol.
Application(s)	Bioinformatics; Food testing; Media testing; Quality control

	Interaction ID	920
	Target type	Cell
	Target unique ID	25922
	Activity	8.0 nM
	Binding Conditions/Buffer	1X PBS, 0.1 mg/mL salmon sperm, 1% bovine serum albumin, and 0.05% Tween-20
	Assay	Binding saturation curve of aptamers
	PubMed ID	31837967

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_648	DNA	GCACACCGATGGCGGTCCTGTTTAGGGGGTGTGC	62.22%
Apta_649	DNA	GCACACCGATGGCGGTCCTGTTTAGGGGGTGTGCGTG	60.00%
Apta_756	DNA	GGGGGACACGGAGGTGGTGGAAAGGCTAAGATTTGATGATGAGTAGTGTGGT	59.62%
Apta_217	DNA	CGCCTGATTAGCGATACTCAGGCGTTGGGTGGGTGGGTGGG	57.78%
Apta_1206	DNA	GGCAGCAGCAATGTAACACTGTGTGTATGTGTTGG	57.78%
Apta_686	DNA	GGTCACCAACAACAGGGAGCGCTACGCGAAGGGTCAATGTGACGTCATGCGGATGTGTGG	56.67%
Apta_1230	DNA	GGCGATAGGCAGTGTTGCGGGGTCGGAGAGCGAGGTAATAGCGTGTATGGGTGCTGTGTG	56.67%
Apta_150	DNA	CCGTAGGTTCGGGGCGGAGTGGTCCGGAAGGTGGCGTGG	55.56%
Apta_677	DNA	CCAAATGCGACGGGGCCTGTTTTAATGGGG	55.56%
Apta_1204	DNA	GGCACGATGTGGCTACATCGATCGCGGTACTGGTG	55.56%
Apta_1204	DNA	GGCACGATGTGGCTACATCGATCGCGGTACTGGTG	55.56%
Apta_694	DNA	ACTCACTATGGAAGAGATGGCGACATCTCTTCTCCGAGCCGGTCGAAATAGTGAGT	55.36%
Apta_223	DNA	CATTGAGATAGCTAGTTGTAGCTGCGTCATAGGCTGGGTTGGGTCTAGTGGTTGGGTGTG	55.00%
Apta_1035	DNA	ATGCGGATCCCGCGCGGGTCTGAGGAGTGCGCGGTGCCAGTGAGTGCGCGAAGCTTGCGC	55.00%
Apta_1172	DNA	GACGCCACTGACTACCCCAGTATCTCTTCAACGCGTATTGAAGTCAGTCGGTCGTCA	54.39%
Apta_650	DNA	GCACACCGATGGCGGTCCTGTTTAGGGGGTGTGCGTGGGGAGGTGCGGTTGC	53.85%
Apta_753	DNA	GGGCACAGACGGAAGATGAGAATTGTGGGGCTTAGTATAGTGAGGTGCGTGT	53.85%
Apta_65	DNA	CTACCGTGGTAGGGAAGGTTGGAGTGTAG	53.33%
Apta_148	DNA	CCGTAGGTTCGGGGTCGGAGTGGTCCGGAAGGTGGCGTGG	53.33%
Apta_149	DNA	CCGTAGGTTCGGGGTCGGAGTGGTCCGGAAGATGGCGTGG	53.33%

ABOUT