Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 922
Aptamer sequence: TGAGCCCAAGCCCTGGTATGTTACAGTATGCTACCTCTACTTGAAGGTTGGTCGACGCGGGGCAGGTCTACTTTGGGATC
Target unique ID: BAA-2587-PACK
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_836 | Apt-1 | Cell | Enterohemorrhagic Escherichia coli O157:H7 (E. coli O157:H7) | 18.97 nM | 1× BB, 50 mmol/L Tris-HCl (pH 7.4), 5 mmol/L KCl, 100 mmol/L NaCl, and 1 mmol/L MgCl2 | 29756774 |
Structure information of aptamer
| Aptamer Sequence: | TGAGCCCAAGCCCTGGTATGTTACAGTATGCTACCTCTACTTGAAGGTTGGTCGACGCGGGGCAGGTCTACTTTGGGATC |
| The optimal secondary structure in dot-bracket notation: | ....(((((((((((((.((.........((((((((.....)).)).)))))))).)))))).........)))))... |
| The centroid secondary structure in dot-bracket notation: | ....(((((((((((..............((((((((.....).)))).))).....)))))).........)))))... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_836 | Description | Apt-1 |
| Aptamer chemistry | DNA | Length | 80 nt |
| GC content | 53.8% | Molecular weight | 24,723.79 Da |
| Molarity of 1 μg/μl solution | 40.45 μM | Number of G-quadruplexes | 2 |
| G-Score | 18 | Function | Targeted binding |
| Sequence | TGAGCCCAAGCCCTGGTATGTTACAGTATGCTACCTCTACTTGAAGGTTGGTCGACGCGGGGCAGGTCTACTTTGGGATC | ||
| Applications | Binding to Enterohemorrhagic Escherichia coli O157:H7 (E. coli O157:H7) | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| ATCC ID | BAA-2587-PACK | Cell name | Enterohemorrhagic Escherichia coli O157:H7 (E. coli O157:H7) |
| Category | Bacteria | Organism | N/A |
| Product type | Reporter-labeled bacterium | Cell type | N/A |
| Morphology | N/A | Tissue | N/A |
| Disease | N/A | Product format | Frozen |
| Antigen expression | N/A | Genes expressed | N/A |
| Cell description | A kit containing reporter-labeled Escherichia coli designed to distinguish control strain cross-contamination from true contamination in test samples. This product is appropriate for use as a positive control in quality control assays for E. coli. |
|
|
| Application(s) | Assay development; Food testing; High-throughput screening | ||
Activity data
 |
Interaction ID | 922 |
| Target type | Cell | |
| Target unique ID | BAA-2587-PACK | |
| Activity | 18.97 nM | |
| Binding Conditions/Buffer | 1× BB, 50 mmol/L Tris-HCl (pH 7.4), 5 mmol/L KCl, 100 mmol/L NaCl, and 1 mmol/L MgCl2 |
|
| Assay | Histogram of the percent gated fluorescence intensity above library background for individual aptamers |
|
| PubMed ID | 29756774 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_844 | DNA | TGAGCCCAAGCCCTGGTATGTTGCGGGAGTCTAGCGGGCCACACTTTTATAGGTTCGCAGGGCAGGTCTACTTTGGGATC | |
| Apta_838 | DNA | TGAGCCCAAGCCCTGGTATGTAGTAATGGTGCGTACAGGCGACGGGGTCCAGGCTGGAGGGGCAGGTCTACTTTGGGATC | |
| Apta_841 | DNA | TGAGCCCAAGCCCTGGTATGGTGTGCCTGTCGTTGTATTGGTCGGTAGGGATCGGAGTGGGGCAGGTCTACTTTGGGATC | |
| Apta_837 | DNA | TGAGCCCAAGCCCTGGTATGTGATCGGTGACGAGGGTGCGGGGCGGGGGGTGAGGCACAGGGCAGGTCTACTTTGGGATC | |
| Apta_842 | DNA | TGAGCCCAAGCCCTGGTATGTGTGGGGTCCTGGATTATGTTTAGCGTCTTTCGCAGTGGGGGCAGGTCTACTTTGGGATC | |
| Apta_843 | DNA | TGAGCCCAAGCCCTGGTATGTCACATCCGGGTTCTTTGCGAACGCGTTTCCGCAGTCTCAGGCAGGTCTACTTTGGGATC | |
| Apta_840 | DNA | TGAGCCCAAGCCCTGGTATGCGGATAACGAGGTATTCACGACTGGTCGTCAGGTATGGTTGGCAGGTCTACTTTGGGATC | |
| Apta_839 | DNA | TGAGCCCAAGCCCTGGTATGAGCCCACGGAACACTGGTCGCGCCCACTGGTTTCTATATTGGCAGGTCTACTTTGGGATC | |
| Apta_568 | DNA | CCCAGCAGCACTGGTAGTGAGGCAGTTCACCGGTGGGGCGGTGAGTTTGGCTGCTATTTAT | |
| Apta_902 | DNA | ATACGGGAGCCAACACCACCAACATTATCAGAGTATGTTACTTATAGTGGTGGCAGAGCAGGTGTGACGGAT | |
| Apta_802 | DNA | ATGAGAAGCGTCGGTGTGGTTAAACACGGAACGAAGGTGCAGGAAGATTTGTCGATGCGGTGCCTGAGCGGGCTGGCAAGGCGCATA | |
| Apta_31 | DNA | ATACCAGTCTATTCAATTGGGCCCGTCCGTATGGTGGGTGTGCTGGCCAGATAGTATGTGCAATCA | |
| Apta_554 | DNA | TGTACCGTCTGAGCGATTCGTACCATTAGTGGGTGCTCCTTACCTGATGGTCATCTAGCCAGTCAGTGTTAAGGAGTGC | |
| Apta_656 | DNA | GCACACCGATGGCGGTCCTGTTTTACGGTAGCTTATATGCAGGGGGTGTGCGTGGGGAGGTGCGGTTGC | |
| Apta_233 | DNA | AGCAGCACAGAGGTCAGATGTGAAACATAGCATATTTACTTATGTCGCCTTGCCGGTTCCTATGCGTGCTACCGTGAA | |
| Apta_234 | DNA | AGCAGCACAGAGGTCAGATGAAATTTTGCACTTACTTGACGTTTCCTCGATAGCCACAAGCCTATGCGTGCTACCGTGAA | |
| Apta_408 | DNA | GCAATGGTACGGTACTTCCGTCATCAGCTTGTGATGTGGATGCGAACTGCAAAAGTGCACGCTACTTTGCTAA | |
| Apta_555 | DNA | TGTACCGTCTGAGCGATTCGTACGGATTGGGTTTGCACTTTACCTGCGGTGCATCGCAGCCAGTCAGTGTTAAGGAGTGC | |
| Apta_888 | DNA | GGCGACCCCCGGGCTACCAGACAATGTACGCAGCAAGAGTGACGGTCGTACCTCGGAGTC | |
| Apta_890 | DNA | GGCGGCCGTGAAATTTGCCAAATGCCGTCTTGGCTTTCGCCCAATGTATCCTGGGTGTTC |
DEV TOOL
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