Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 321
Aptamer sequence: GGGAAAAGAGACGGCCGGCGCCAUAGCCGAGAUCCGAGGUGUUGCCGAGAA
Target unique ID: P14555
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_312 | Aptamer 15 | Protein | PA2GA_HUMAN | 1.7 nM | Tris-buffered saline (25 mM Tris, pH 7.4, 150 mM NaCl); Tris potassium calcium buffer (25 mM Tris, 2.5 mM CaCl2, 100 mM KCl) | 9526554 |
Structure information of aptamer
| Aptamer Sequence: | GGGAAAAGAGACGGCCGGCGCCAUAGCCGAGAUCCGAGGUGUUGCCGAGAA |
| The optimal secondary structure in dot-bracket notation: | ...........((((.((((((...............)))))))))).... |
| The centroid secondary structure in dot-bracket notation: | ...........(((..((((((...............)))))).))).... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_312 | Description | Aptamer 15 |
| Aptamer chemistry | RNA | Length | 51 nt |
| GC content | 62.7% | Molecular weight | 16,883.98 Da |
| Molarity of 1 μg/μl solution | 59.23 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted therapy |
| Sequence | GGGAAAAGAGACGGCCGGCGCCAUAGCCGAGAUCCGAGGUGUUGCCGAGAA | ||
| Applications | Inhibits enzymatic activity | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P14555 | Protein name | Phospholipase A2, membrane associated (EC 3.1.1.4) (GIIC sPLA2) (Group IIA phospholipase A2) (Non-pancreatic secretory phospholipase A2) (NPS-PLA2) (Phosphatidylcholine 2-acylhydrolase 2A) |
| Gene name(s) | PLA2G2A PLA2B PLA2L RASF-A | Organism | Phospholipase A2, membrane associated (EC 3.1.1.4) (GIIC sPLA2) (Group IIA phospholipase A2) (Non-pancreatic secretory phospholipase A2) (NPS-PLA2) (Phosphatidylcholine 2-acylhydrolase 2A) |
| Status | reviewed in uniprot database | Length | 144 |
| Mass | 16,083 | Activity regulation | N/A |
| Involvement in disease | N/A | Catalytic activity | Reaction=a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + H2O = a 1-acyl-sn-glycero-3-phosphoethanolamine + a fatty acid + H(+) |
| PDB ID(s) | 1AYP;1BBC;1DB4;1DB5;1DCY;1J1A;1KQU;1KVO;1N28;1N29;1POD;1POE;3U8B;3U8D;3U8H;3U8I;5G3N; |
 |
Function | Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids with implications in host antimicrobial defense, inflammatory response and tissue regeneration. |
Activity data
 |
Interaction ID | 321 |
| Target type | Protein | |
| Target unique ID | P14555 | |
| Activity | 1.7 nM | |
| Binding Conditions/Buffer | Tris-buffered saline (25 mM Tris, pH 7.4, 150 mM NaCl); Tris potassium calcium buffer (25 mM Tris, 2.5 mM CaCl2, 100 mM KCl) |
|
| Assay | Arachidonic acid-mediated contractions of guinea pig lung pleural strips in the presence of aptamer 15 |
|
| PubMed ID | 9526554 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_230 | RNA | GGGAGGACGAUGCGGGCGUGAAUGGUGUUGUGAGG | |
| Apta_370 | RNA | GGGAGGACGCGUCGCCGUAAUGGAUGUUUUGCUCCCUG | |
| Apta_645 | RNA | CUGGACGACGGGGACGCCACUGGACUAGGUAAGCCAGGACCGUACGUCGGGAGCCGUCAGAAUA | |
| Apta_1168 | RNA | GCGACUACGGUGAGGGUCGGGUCCAGUAGCUUCGGCUACUGUUGAGUAGAGUGUGGGCUCCGUAGUCGC | |
| Apta_33 | DNA | GCGGTAAACTGCGTCCGAAGGGGCTGCAGTGACCCGAATGGGTCCGCCGCGAAGCG | |
| Apta_983 | DNA | ATGGGAACAGAGCGGCGCAGAGACCCAATGCCACGAGTACCTGACCCTATCAACCCGTGA | |
| Apta_660 | RNA | UGGGAGGAGUGCUGCUCUCCAGGCACUCUUGUUGCUCGGA | |
| Apta_153 | RNA | CCACAGUUCAGAGGCACCGCGAACAAAACGCAAGACAGAGUGCCGACAAGAAC | |
| Apta_130 | RNA | GGGAGGACGAUGCGGUGGUAGCGCGAUAUAGCGCUGGUAGGGUUGCCGGUGAUCAGACGACUCGCCCGA | |
| Apta_793 | RNA | GGGAGAGAACAAUGACCUGCGGUGCCAAGCCGUCGGGUUAUGUUGAUCUCCUCAAGGACGAGUGCAUUGCAUCACGUCAGUAG | |
| Apta_398 | RNA | GGGAGGACGAUGCGGCUUUAAUCCUCGCACUCAGCGCGCAUCACCCUUGACAUCACAGACGACUCGCUGAGGAUCCGAGA | |
| Apta_131 | RNA | GGGAGGACGAUGCGGUCCUGUCGUCUGUUCGUCCCCAGACGACUCGCCCGA | |
| Apta_134 | RNA | GGGAGGACGAUGCGGGCCGUUGUUGUGUGCCGCCCCAGACGACUCGCCCGA | |
| Apta_315 | RNA | GGGAGGACGAUGCGGUCGUAUUAUCCGCUGCACGCCAGACGACUCGCCCGA | |
| Apta_366 | RNA | GGUUAGAGGCGACGUCCCGGAAUCCGCGCCCAUUGCCCCC | |
| Apta_703 | RNA | GGGCGGAUGAGACGCUUGGCGUGUGCUGUGGAGAGUCAUCCG | |
| Apta_1288 | RNA | GGCGCACUGGCGCUGCGCCUUCGGGCGCCAAUCGUAGCGUGUCGGCGCC | |
| Apta_129 | RNA | GGGAGGACGAUGCGGACACCGUUAAUCUGAGGCCCUGUCCUAUUCCUUCACGCCUCAGA | |
| Apta_984 | RNA | GGGAGGACGAUGCGGCGUAUUGCGCGAGGAUUAUCCGCUCAUCGUUGUUGUUGUGCAGACGACUCGCCCGA | |
| Apta_38 | RNA | GGUAGAUACGAUGGAUACCCCCUGUGGCCCGUCAACACAGGGGAAGUGGCAUGACGCGCAGCCA |
DEV TOOL
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