Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 230
Aptamer sequence: GGGAGATCACTTACGGCACCGTATAGTCGTACATGAA-CGAGTGTGAGTGTTAAGCCTTTG-GCGACAGGGCTCGGAACC
Target unique ID: P05412
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_226 Aptamer-19 Protein JUN_HUMAN 0.5 nM 20 mM Tris (pH 7.9), 0.1 mM EDTA, 10% glycerol, 1 mM DTT, 150 mM KCl, 5 mM MgCl2, 0.04% NP-40 24968026
Structure information of aptamer
Aptamer Sequence: GGGAGATCACTTACGGCACCGTATAGTCGTACATGAA-CGAGTGTGAGTGTTAAGCCTTTG-GCGACAGGGCTCGGAACC
The optimal secondary structure in dot-bracket notation: ......(((..((((((........))))))..))).(((((.....((((..(((...)))))))..))))).....
The centroid secondary structure in dot-bracket notation: ......(((..((((((........))))))..))).(((((...........(((...)))......))))).....
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_226 Description Aptamer-19
Aptamer chemistry DNA Length 80 nt
GC content 53.8% Molecular weight 24,446.43 Da
Molarity of 1 μg/μl solution 40.91 μM Number of G-quadruplexes No QGRS found
G-Score N/A Function Targeted binding
Sequence GGGAGATCACTTACGGCACCGTATAGTCGTACATGAA-CGAGTGTGAGTGTTAAGCCTTTG-GCGACAGGGCTCGGAACC
Applications Binding to target proteins
Target information
Type Detail Type Detail
Uniprot ID P05412 Protein name Transcription factor Jun (Activator protein 1) (AP1) (Proto-oncogene c-Jun) (Transcription factor AP-1 subunit Jun) (V-jun avian sarcoma virus 17 oncogene homolog) (p39)
Gene name(s) JUN Organism Transcription factor Jun (Activator protein 1) (AP1) (Proto-oncogene c-Jun) (Transcription factor AP-1 subunit Jun) (V-jun avian sarcoma virus 17 oncogene homolog) (p39)
Status reviewed in uniprot database Length 331
Mass 35,676 Activity regulation N/A
Involvement in disease N/A Catalytic activity N/A
PDB ID(s)
1A02;1FOS;1JNM;1JUN;1S9K;1T2K;5FV8;5T01;6Y3V;
Function Transcription factor that recognizes and binds to the AP-1 consensus motif 5'-TGA[GC]TCA-3'.
Activity data
Interaction ID 230
Target type Protein
Target unique ID P05412
Activity 0.5 nM
Binding Conditions/Buffer

20 mM Tris (pH 7.9), 0.1 mM EDTA, 10% glycerol, 1 mM DTT, 150 mM KCl, 5 mM MgCl2, 0.04% NP-40
Assay

Aptamer-19(12–74) is 100-fold more specific for binding cJun/cJun compared to cJun/cFos. Data from EMSAs were quantified and fit to binding curves; the equilibrium dissociation constant of the aptamer for cJun/cJun is 0.5 nM and for cJun/cFos is 0.85 nM
PubMed ID 24968026
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_1218 DNA CGACACCACACAGCAGTAAGCACTACAACATAGTGGTCTAGACTATCTCGGGCCGGTG 53.75%
Apta_1133 DNA GCCGAGATTGCACTTACTATCTAAAGAAGAGTAGTAAGAGGCGTGGGTGATTATGGGTAAGTTGTATGAGGTAATTGAATAAGCTGGTATGCGC 53.19%
Apta_933 DNA GACGCTTACTCAGGTGTGACTCGGGGTGTAGCTCGTTATGATTCGGACAAGACTTACCTTGCGCCTCTGGGATCGAAGGACGCAGATGAAGTCTC 52.63%
Apta_417 DNA GGCAGGAAGACAAACACCAGGTGAGGGAGACGACGCGAGTGTTAGATGGTAGCTGTTGGTCTGTGGTGCTGT 52.50%
Apta_422 DNA GGGAGCTCAGAATAAACGCTCAATTCGGGAATGATTATCAAATTTATGCCCTCTGATTTCGACATGAGGCCCGGATC 52.50%
Apta_810 DNA ACAGCATCCCCATGTGAACAATCGCATTGTGATTGTTACGGTTTCCGCCTCATGGACGTGCTG 52.50%
Apta_891 DNA GGAGACCGTACCATCTGTTCGTGGAAGCGCTTTGCTCGTCCATTAGCCTTGTGCTCGTGC 52.50%
Apta_1130 DNA GCCGAGATTGCACTTACTATCTATTAGGAATAAGGTATGAATAGAGATGTATAAGTTGTGTGGACGGTGTAATTGAATAAGCTGGTATGCGC 52.17%
Apta_1137 DNA GCCGAGATTGCACTTACTATCTCAAGAAGAGTAGTAAGAGGAGTGGGTGGTTATGAGTAAGTTGTATGTGGTAATTGAATAAGCTGGTATGCGC 52.13%
Apta_490 DNA GGGAGACAAGAATAAACGCTCAAGGGCGTGGTGGGTGGGGTACTAATAATGTGCGTTTGTTCGACAGGAGGCTCACAACAGGC 51.81%
Apta_1135 DNA GCCGAGATTGCACTTACTATCTCATCAATAAAGATTATAGGGTGAAGGTGATAGTAGGTGGTATATAGGGTAATTGAATAAGCTGGTATGCGC 51.61%
Apta_246 DNA GGCTTTACACTTTATGCTTCCGGCCCGCGGAAATGATAACGCGTATGGTGGTATAACAATTTCACACAGGAAACAG 51.25%
Apta_501 DNA GGGAGACAAGAATAAACGCTCAATTTAACGTATTTATTCAAGCTCGTATTCGACAGGAGGCTCACAACAGGC 51.25%
Apta_536 DNA ATCCGTCACACCTGCTCTCGAATAGAGTACTCGATACTGACTTTATTTACCGTCTGGTGTTGGCTCCCGTAT 51.25%
Apta_615 DNA ACGAAGAAACAGGGCGCTTCGAACGAACATGGGCAGGGTGGGAAGTTTTAAAGTGGTAC 51.25%
Apta_618 DNA GCGGCAAATCAGCATACGAGTGTACAGGGACGGGACGGGTGGGTAAAAGGTGTCGCCTCAG 51.25%
Apta_686 DNA GGTCACCAACAACAGGGAGCGCTACGCGAAGGGTCAATGTGACGTCATGCGGATGTGTGG 51.25%
Apta_807 DNA GGGTCTTCCTGGACTGTCGAAAATTCAGTATCGGGAGGTTACGTATTTGGTTTATAGATAGTAA 51.25%
Apta_920 DNA ATCCAGAGTGACGCAGCACGGCACTCACTCTTTGTTAAGTGGTCTGCTTCTTAACCTTCATCGACACGGTGGCTTA 51.25%
Apta_1111 DNA GTGCGTCGCATGATCGCGATTAGTGCGACCATGTACGATTCTCACGACAACGACACTGTACTTGAGGACTGTCTCC 51.25%

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AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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