AptaDB: Result

Interaction ID: 370

Aptamer sequence: GGACCUAUGCAGUAGCCAGUGUGGACUGGGCUGCCCCCCC

Target unique ID: P23284

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_365	M9-5	Protein	PPIB_HUMAN	1.5 nM	20 mM HEPES pH 7.5, 150 mM NaCl, 2mM CaCl2, and 3 mM MgCl2	22484812

Aptamer Sequence:	GGACCUAUGCAGUAGCCAGUGUGGACUGGGCUGCCCCCCC
The optimal secondary structure in dot-bracket notation:	........(((((..(((((....))))))))))......
The centroid secondary structure in dot-bracket notation:	........(((((..(((((....))))))))))......

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_365	Description	M9-5
Aptamer chemistry	RNA	Length	40 nt
GC content	67.5%	Molecular weight	13,056.58 Da
Molarity of 1 μg/μl solution	76.59 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted therapy
Sequence	GGACCUAUGCAGUAGCCAGUGUGGACUGGGCUGCCCCCCC
Applications	Discriminated between the sera of pancreatic cancer patients and healthy volunteers with high sensitivity and specificity

Type	Detail	Type	Detail
Uniprot ID	P23284	Protein name	Peptidyl-prolyl cis-trans isomerase B (PPIase B) (EC 5.2.1.8) (CYP-S1) (Cyclophilin B) (Rotamase B) (S-cyclophilin) (SCYLP)
Gene name(s)	PPIB CYPB	Organism	Peptidyl-prolyl cis-trans isomerase B (PPIase B) (EC 5.2.1.8) (CYP-S1) (Cyclophilin B) (Rotamase B) (S-cyclophilin) (SCYLP)
Status	reviewed in uniprot database	Length	216
Mass	23,743	Activity regulation	Inhibited by cyclosporin A (CsA)
Involvement in disease	Osteogenesis imperfecta 9 (OI9)	Catalytic activity	Reaction=[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)
PDB ID(s)	1CYN;3ICH;3ICI;
Function	PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding.

No relevant experimental diagram	Interaction ID	370
	Target type	Protein
	Target unique ID	P23284
	Activity	1.5 nM
	Binding Conditions/Buffer	20 mM HEPES pH 7.5, 150 mM NaCl, 2mM CaCl2, and 3 mM MgCl2
	Assay	N/A
	PubMed ID	22484812

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_313	RNA	UGCCCUGCCCUCACCCGUUAGCCUGAGCGCCCCGCA	60.00%
Apta_370	RNA	GGGAGGACGCGUCGCCGUAAUGGAUGUUUUGCUCCCUG	57.50%
Apta_521	RNA	GGGUUGGGAAGAAACUGUGGCACUUCGGUGCCAGCAACCC	55.00%
Apta_856	RNA	UGGGCCGGAGGUUAGCUUGCCCAUGGCAAGCAGGGCGCCACGGACCCA	54.17%
Apta_647	RNA	GGAUCCCGACUGGCGAGAGCCAGGUAACGAAUGGAUCC	52.50%
Apta_647	RNA	GGAUCCCGACUGGCGAGAGCCAGGUAACGAAUGGAUCC	52.50%
Apta_1167	RNA	GGCGCGAGGAAGGAGGUCUGAGGAGGUCACUGCGCC	52.50%
Apta_1292	RNA	GGCACGUGUAUUACCCUAGUGGUCGACGUGCC	52.50%
Apta_703	RNA	GGGCGGAUGAGACGCUUGGCGUGUGCUGUGGAGAGUCAUCCG	52.38%
Apta_243	RNA	GCUUGAAGCUAGCAGUAAGACUAGCGCUACGGAUGGGUGUGCG	51.16%
Apta_636	DNA	TAGCCGGATCGCGCTGGCCAGATGATATAAAGGGTCAGCCCCCCA	51.11%
Apta_668	RNA	GGAACCCAACUAGGCGUUUGAGGGGAUUCGGCCACGGUAACAACCCCUC	51.02%
Apta_2	RNA	GGGCUUCUCUGGUUAGACCAGAUUUGAGCCUGGGAGCUCUCUGGCUAACUAGGGAACCC	50.85%
Apta_1248	RNA	GGGUGACUUUUCCCUCAGGCUCCUGUGAAGCAACCGAAUGGACUGAAAUGAAACCGCCC	50.85%
Apta_496	RNA	GGGCUAGCUGAUCGUACCCAGUAGCGUGGCAUGGGGUGCCUAGUCGGGCGAUACUAACAGCUAACACCC	50.72%
Apta_71	DNA	GGCGAGTAGGTTGGTGTGGTTGGGGCTCGCC	50.00%
Apta_73	DNA	GGGGAGTAGGTTGGTGTGGTTGGGGCTCCCC	50.00%
Apta_74	DNA	GGACAGTAGGTTGGTGTGGTTGGGGCTGTCC	50.00%
Apta_95	RNA	CCGCGACAGUCGUAAGUUUUGACUGACUGAACGUU	50.00%
Apta_431	RNA	CAGGCAUGCCUAGCUAAGCAGCCCAUGGCUUAUGCGCGGAAUAUUGGCUUCCGUUC	50.00%

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