Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1168
Aptamer sequence: ATCCAGAGTGACGCAGCAGGGCACATTGTTCACACACAGATCACATTACGGAAAACACAACTACACGAAATGTCGTTGGTGGCCC
Target unique ID: CRL-1427
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_1112 | OS-7.9 | Cell | MG-63 | 12.8 nM | PBS with 4.5 mg/mL glucose [Acros], 5 mM MgCl2 [VWR], 1 mg/mL bovine serum albumin (BSA) | 34383183 |
Structure information of aptamer
| Aptamer Sequence: | ATCCAGAGTGACGCAGCAGGGCACATTGTTCACACACAGATCACATTACGGAAAACACAACTACACGAAATGTCGTTGGTGGCCC |
| The optimal secondary structure in dot-bracket notation: | .......((((....((((......)))))))).(((.(((.(((((.((...............)).))))).))).))).... |
| The centroid secondary structure in dot-bracket notation: | .......(((....................))).(((.(((.(((((.((...............)).))))).))).))).... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_1112 | Description | OS-7.9 |
| Aptamer chemistry | DNA | Length | 85 nt |
| GC content | 49.4% | Molecular weight | 26,176.82 Da |
| Molarity of 1 μg/μl solution | 38.20 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted therapy |
| Sequence | ATCCAGAGTGACGCAGCAGGGCACATTGTTCACACACAGATCACATTACGGAAAACACAACTACACGAAATGTCGTTGGTGGCCC | ||
| Applications | OS-7.9 could recognize a common protein expressed in these cancer cells, possibly becoming a potential molecular probe for early diagnosis and targeted therapies for metastatic disease | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| ATCC ID | CRL-1427 | Cell name | MG-63 |
| Category | Human cells | Organism | Homo sapiens, human |
| Product type | N/A | Cell type | N/A |
| Morphology | fibroblast | Tissue | Bone |
| Disease | Osteosarcoma | Product format | Frozen |
| Antigen expression | N/A | Genes expressed | interferon |
| Cell description | N/A |
|
|
| Application(s) | 3D cell culture | ||
Activity data
 |
Interaction ID | 1168 |
| Target type | Cell | |
| Target unique ID | CRL-1427 | |
| Activity | 12.8 nM | |
| Binding Conditions/Buffer | PBS with 4.5 mg/mL glucose [Acros], 5 mM MgCl2 [VWR], 1 mg/mL bovine serum albumin (BSA) |
|
| Assay | Binding curve of OS-7.9 aptamer to target MG-63 cells and negative control MC3T3-E1 cells |
|
| PubMed ID | 34383183 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_921 | DNA | ATCCAGAGTGACGCAGCACGAGCCAGACATCTCACACCTGTTGCATATACATTTTGCATGGACACGGTGGCTTAGT | |
| Apta_1293 | DNA | ATCCAGAGTGACGCAGCATGCCCTAGTTACTACTACTCTTTTTAGCAAACGCCCTCGCTTTGGACACGGTGGCTTAGT | |
| Apta_920 | DNA | ATCCAGAGTGACGCAGCACGGCACTCACTCTTTGTTAAGTGGTCTGCTTCTTAACCTTCATCGACACGGTGGCTTA | |
| Apta_923 | DNA | ATCCAGAGTGACGCAGCATCGACCTCTGTGCGAGCGGGTTGCATATATATTTTGCATGGACACGGTGGCTTAGT | |
| Apta_528 | DNA | ATACGGGAGCCAACACCACTTGGTTAACCAATCAAACCGGACAACGAAGGGTCCAGAGCAGGTGTGACGGAT | |
| Apta_922 | DNA | ATCCAGAGTGACGCAGCACACCGTTTGTATTCTGCATTGTTTTGCATTCTACATGGACACGGTGGCTTAGT | |
| Apta_1090 | DNA | ATCCAGAGTGACGCAGCAGGGCTTGGGTTGGGAATAAGGATGTGGGAGGCGGCGAACATGGACACGGTGGCTTAGT | |
| Apta_458 | DNA | AGCTCCAGAAGATAAATTACAGGGAGAGTAAACTTTGCAAACACAACAATACCATTCCGGAACGTTCTTAACACAACTAGGATACTATGACCCC | |
| Apta_794 | DNA | ATCCAGAGTGACGCAGCAAGGAGATTCGAGGGGGAAGGTTTGTTATAGGGGTTAATGGACACGGTGGCTTAGT | |
| Apta_401 | DNA | ATCCAGAGTGACGCAGCAGATAAGTAGGTATCCGTTTGAAAAACTTTTCTGACCGTCCGACTATGGACACGGTGGCTTAGT | |
| Apta_784 | DNA | ATCCAGAGTGACGCAGCATTTTGACGCTTTATCCTTTTCTTATGGCGGGATAGTTTCGTGGACACGGTGGCTTAGT | |
| Apta_786 | DNA | ATCCAGAGTGACGCAGCATTTGTATCCTATTTTGTTTATGTAATTGTCGTTGATCATGTGGACACGGTGGCTTAGT | |
| Apta_1049 | DNA | ATACGGGAGCCAACACCATGTACATTGTAGATTTATTTGCCCAAAACCCAAGAAGAGCAGGTGTGACGGAT | |
| Apta_457 | DNA | AGCTCCAGAAGATAAATTACAGGGGATCAGTAAGGTTGAGACGGCCTGAATCTATCGTGGAGACCACGCGACGCAACTAGGATACTATGACCCC | |
| Apta_288 | DNA | ATCCAGAGTGACGCAGCATTTCATCGGGTCCAAAAGGGGCTGCTCGGGATTGCGGATATGGACACGT | |
| Apta_600 | DNA | GTCTCTGTGTGCGCCAGAGAACACTGGGGCAGATATGGGCCAGCACAGAATGAGGCCC | |
| Apta_689 | DNA | CCACACAACAGCCTACGTGGATACACCACATACATCCCATAACCCCGTGTCATGTGTCG | |
| Apta_785 | DNA | ATCCAGAGTGACGCAGCATGAATCTTTTCTTTTGGTTTTGATATTTATAGTTGGTGAATGGACACGGTGGCTTAGT | |
| Apta_944 | DNA | ATACGACTCACTATTAGGGACAAAGCTGACAACCCTTTCATAATCTAACTACATTTATGTGCTAGACTACTGACTAC | |
| Apta_992 | DNA | TCACTGACAGTAGGCCCATACGCACATTCAACAATCGGTAGTCCATACAACCGCGCCCCGTG |
DEV TOOL
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