Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 38
Aptamer sequence: GGUAGAUACGAUGGAUACCCCCUGUGGCCCGUCAACACAGGGGAAGUGGCAUGACGCGCAGCCA
Target unique ID: O15455
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_38 | Family-1 | Protein | TLR3_HUMAN | 2.1 nM | 2 mM HEPES-NaOH (pH 7.6), 3 mM MgCl2, and 100 mM NaCl | 17150912 |
Structure information of aptamer
| Aptamer Sequence: | GGUAGAUACGAUGGAUACCCCCUGUGGCCCGUCAACACAGGGGAAGUGGCAUGACGCGCAGCCA |
| The optimal secondary structure in dot-bracket notation: | ..................((((((((.........))))))))...((((.((.....)))))) |
| The centroid secondary structure in dot-bracket notation: | ..................((((((((.........))))))))....(((.((.....))))). |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_38 | Description | Family-1 |
| Aptamer chemistry | RNA | Length | 64 nt |
| GC content | 60.9% | Molecular weight | 19,805.68 Da |
| Molarity of 1 μg/μl solution | 50.49 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted binding |
| Sequence | GGUAGAUACGAUGGAUACCCCCUGUGGCCCGUCAACACAGGGGAAGUGGCAUGACGCGCAGCCA | ||
| Applications | Binding to TLR3 ECD | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | O15455 | Protein name | Toll-like receptor 3 (CD antigen CD283) |
| Gene name(s) | TLR3 | Organism | Toll-like receptor 3 (CD antigen CD283) |
| Status | reviewed in uniprot database | Length | 904 |
| Mass | 103,829 | Activity regulation | N/A |
| Involvement in disease | Immunodeficiency 83, susceptibility to viral infections (IMD83) | Catalytic activity | N/A |
| PDB ID(s) | 1ZIW;2A0Z;2MK9;2MKA;3ULU;3ULV;5GS0;7C76; |
 |
Function | Key component of innate and adaptive immunity. |
Activity data
 |
Interaction ID | 38 |
| Target type | Protein | |
| Target unique ID | O15455 | |
| Activity | 2.1 nM | |
| Binding Conditions/Buffer | 2 mM HEPES-NaOH (pH 7.6), 3 mM MgCl2, and 100 mM NaCl |
|
| Assay | Binding curves of selected RNA aptamers for TLR3-ECD. Data points were obtained by a filter binding assay (Family-II closed squares] and Family-II [closed trianglesJ). The values represent means of three independent experiments. RNA binding shows the percentage of input RNA retained on the filter in protein/RNA complex |
|
| PubMed ID | 17150912 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_39 | RNA | GGUAGAUACGAUGGACUACCGCCACCCCGGGUCCGGGUGACGUUAAUUGAGGGCCCAUGACGCGCAGCCA | |
| Apta_305 | RNA | GGGAGGACGAUGCGGAUCGCCCUGAACCGGCCCAGCAGACUGCUGACGGCACGAUCCGCAUCGUCCUCCC | |
| Apta_400 | RNA | GGGAGGACGAUGCGGAUACCAGCGAAUAACUCGCUGAGGAACCCGACUCACAAACAGACGACUCGCUGAGGAUCCGAGA | |
| Apta_561 | RNA | GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGCCUGUGGAAACAGACGUGGCACAUGACUACGUCGAAACGGUAGCGAGAGCUC | |
| Apta_397 | RNA | GGGAGGACGAUGCGGAUACCAGGAUCACAUCCUGAGGAACCCCGGCUCCCAACCUCAGACGACUCGCUGAGGAUCCGAGA | |
| Apta_566 | RNA | GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGCCCUGCGAUGCAGAAAGGUGCUGACGACACAUCGAAACGGUAGCGAGAGCUC | |
| Apta_613 | RNA | GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGCCUUAGGAUAUGCAUGAUGCAGAAGGACGUCGAAACGGUAGCGAGAGCUC | |
| Apta_171 | RNA | GGGAGCUCAGAAUAAACGCUCAACCAGUCUUGUGGCUUUGAAAGAGAGGAGUGUUCGACAUGAGGCCCGGAUCCGGC | |
| Apta_399 | RNA | GGGAGGACGAUGCGGCAAACCAGCUAUUUCCUGAGGUACCCCGGCUCUCCAUGGCAGACGACUCGCUGAGGAUCCGAGA | |
| Apta_132 | RNA | GGGAGGACGAUGCGGCGUUUCCUCUGGUUCGUCCCCAGACGACUCGCCCGA | |
| Apta_304 | RNA | CGGCCGAUAGAAAGACUACUUGAGCCCUUAAAUGAGGUUAUGUGCGGCCG | |
| Apta_936 | RNA | GGAGCUCAGCCUUCACUGCGAUCACCGUGUCGGUGGCUAGUCGAUGUAUCCCGAUCACCAUUGGGGGCAAGUUUAGACAGGCACCACGGUCGGAUCCAC | |
| Apta_398 | RNA | GGGAGGACGAUGCGGCUUUAAUCCUCGCACUCAGCGCGCAUCACCCUUGACAUCACAGACGACUCGCUGAGGAUCCGAGA | |
| Apta_415 | RNA | GGGAGGACGAUGCGGAAACUCAUCGGUAGCCUUCCUGCGGUCAGUCUAUUAGGACCAGACGACUCGCUGAGGAUCCGAGA | |
| Apta_467 | RNA | GGGAGGACGAUGCGGCAGUGCCCAACCGGAACAACAACCACCGGCGGCUCCUGCUCAGACGACUCGCUGAGGAUCCGAGA | |
| Apta_562 | RNA | GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGCCUUUAGGGCCAAGUGUGGUGAAAGACACACUCGAAACGGUAGCGAGAGCUC | |
| Apta_352 | RNA | GGGAGGACGAUGCGGUGGUCUCCCAAUUCUAAACUUUCUCCAUCGUAUCUGGGCAGACGACUCGCCCGA | |
| Apta_984 | RNA | GGGAGGACGAUGCGGCGUAUUGCGCGAGGAUUAUCCGCUCAUCGUUGUUGUUGUGCAGACGACUCGCCCGA | |
| Apta_256 | RNA | GGGCGCUAAGUCCUCGCUCAUGCGCGUCCCAUGGGGUAUAGAGGGGUCGAAGUGGACGCGCGACUCGGAUCCU | |
| Apta_131 | RNA | GGGAGGACGAUGCGGUCCUGUCGUCUGUUCGUCCCCAGACGACUCGCCCGA |
DEV TOOL
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