AptaDB: Result

Interaction ID: 318

Aptamer sequence: TAGCCAAGGTAACCAGTACAAGGTGCTAAACGTAATGGCTTCGGCTTAC

Target unique ID: P14151

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_309	LS Aptamer	Protein	LYAM1_HUMAN	3 nM	20 mM Hepes (pH 7.5), 125 mM NaCl, 1 mM CaCl2, 1 mM MgCl2, 5 mM KCl plus 0.01% (wt/vol) human serum albumin	8981912

Aptamer Sequence:	TAGCCAAGGTAACCAGTACAAGGTGCTAAACGTAATGGCTTCGGCTTAC
The optimal secondary structure in dot-bracket notation:	.(((((........(((((...)))))........))))).........
The centroid secondary structure in dot-bracket notation:	.((((.....................................))))...

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_309	Description	LS Aptamer
Aptamer chemistry	DNA	Length	49 nt
GC content	46.9%	Molecular weight	15,353.67 Da
Molarity of 1 μg/μl solution	65.13 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted therapy
Sequence	TAGCCAAGGTAACCAGTACAAGGTGCTAAACGTAATGGCTTCGGCTTAC
Applications	Inhibit L-selectin binding to immobilized SLex in static assays and inhibit L-selectin-mediated rolling of human lymphocytes and neutrophils on cytokine-activated endothelial cells in flow-based assays

Type	Detail	Type	Detail
Uniprot ID	P14151	Protein name	L-selectin (CD62 antigen-like family member L) (Leukocyte adhesion molecule 1) (LAM-1) (Leukocyte surface antigen Leu-8) (Leukocyte-endothelial cell adhesion molecule 1) (LECAM1) (Lymph node homing receptor) (TQ1) (gp90-MEL) (CD antigen CD62L)
Gene name(s)	SELL LNHR LYAM1	Organism	L-selectin (CD62 antigen-like family member L) (Leukocyte adhesion molecule 1) (LAM-1) (Leukocyte surface antigen Leu-8) (Leukocyte-endothelial cell adhesion molecule 1) (LECAM1) (Lymph node homing receptor) (TQ1) (gp90-MEL) (CD antigen CD62L)
Status	reviewed in uniprot database	Length	372
Mass	42,187	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	2LGF;3CFW;5VC1;
Function	Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells.

No relevant experimental diagram	Interaction ID	318
	Target type	Protein
	Target unique ID	P14151
	Activity	3 nM
	Binding Conditions/Buffer	20 mM Hepes (pH 7.5), 125 mM NaCl, 1 mM CaCl2, 1 mM MgCl2, 5 mM KCl plus 0.01% (wt/vol) human serum albumin
	Assay	N/A
	PubMed ID	8981912

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_309	DNA	TAGCCAAGGTAACCAGTACAAGGTGCTAAACGTAATGGCTTCGGCTTAC	100.00%
Apta_306	DNA	CAAGGTAACCAGTACAAGGTGCTAAACGTAATGGCTTCG	79.59%
Apta_939	DNA	TTGCCCACCGTACTGTGCAGGTCGAACTACAGGCACGTCGTG	57.14%
Apta_788	DNA	TACCAGTGCGATGCTCAGTAACTTTGAAGGAAAGGCTACAAACTCTTCCTGACGCATTCGGTTGAC	56.06%
Apta_875	DNA	GCCGCGACCATACAACGCAAACAACACGCCTGTACGCTTG	55.10%
Apta_971	DNA	TAGTCTAGGAACGGTACGATCGTATTCCAAGGACTTTGCTGGTGGTATCC	54.00%
Apta_1047	DNA	TAGTCCCAAGGCACATAAGGGCCGGAGGTCTAGCGTCAGGGATACAGAGA	54.00%
Apta_220	DNA	ATCAACTCACCGTAGGTCTAGTGAGAACTTCAAGTCTACT	53.06%
Apta_445	DNA	AGCCATGACGATGTCGTTACGTAGATGTAGAGACTCCTAA	53.06%
Apta_446	DNA	AGCCATGACGATGTCGTTACGTAGATGCATAGACTCCTAA	53.06%
Apta_642	DNA	ATCCAATACCTCAGAACTCAGTTCGAGTCGTAAAGGGGAATCGCA	53.06%
Apta_835	DNA	TATGGCGTGGCAAGCTTGGCCCGCTTCTCAAGCATGGTTATCTAC	53.06%
Apta_946	DNA	TACCGGGTAATATGCACACATGCTGATAGCAAGCAATGCA	53.06%
Apta_1032	DNA	TGGCAAGAGGGTGTGCTTAAGGTGGACACGGTGGCTTAG	53.06%
Apta_1271	DNA	ACCACAGCCAGTAAGTCCCGTAGGCCCTGGTTGGGCTGTT	53.06%
Apta_1172	DNA	GACGCCACTGACTACCCCAGTATCTCTTCAACGCGTATTGAAGTCAGTCGGTCGTCA	52.63%
Apta_570	DNA	GGCAAGATGTGCCCAGAACAGTTGCTTGTATGGTGGGGAGCGTCCATATTGGCTTAAAC	52.54%
Apta_176	DNA	AGCAGCACAGAGGTCAGATGTATGGTACATTCGAGCGAGCTAAACTAGGATTAAACTCACCCTATGCGTGCTACCGTGAA	52.50%
Apta_1173	DNA	TTGACTTGCCACTGACTACCCCAGTATCTCTTCAACGCTATGGTGCATGTACTACGTATTGAAGTCAGTCGGTCGTCATC	52.50%
Apta_395	DNA	AATCCACGTATCGACTAGCTCAAACCAGCCTGATAGAGCCATTCGCTTTA	52.00%

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