Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1173
Aptamer sequence: CGTACGGAATTCGCTAGCGGACTGGAGTCTAGACCGGGTAGCTGTGGTGGATCCGAGCTCCACGTG
Target unique ID: P43490
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_1117 G55 Protein NAMPT_HUMAN 71.7 nM Tris-HCl 20 mM, pH 7.4 22143382
Structure information of aptamer
Aptamer Sequence: CGTACGGAATTCGCTAGCGGACTGGAGTCTAGACCGGGTAGCTGTGGTGGATCCGAGCTCCACGTG
The optimal secondary structure in dot-bracket notation: .((.((((.(((((((((...((((........))))...)))..)))))))))).))........
The centroid secondary structure in dot-bracket notation: .((.((((.(((((((((...((((........))))...)))..)))))))))).))........
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_1117 Description G55
Aptamer chemistry DNA Length 66 nt
GC content 60.6% Molecular weight 20,483.07 Da
Molarity of 1 μg/μl solution 48.82 μM Number of G-quadruplexes No QGRS found
G-Score N/A Function Targeted binding
Sequence CGTACGGAATTCGCTAGCGGACTGGAGTCTAGACCGGGTAGCTGTGGTGGATCCGAGCTCCACGTG
Applications Binding to nampt
Target information
Type Detail Type Detail
Uniprot ID P43490 Protein name Nicotinamide phosphoribosyltransferase (NAmPRTase) (Nampt) (EC 2.4.2.12) (Pre-B-cell colony-enhancing factor 1) (Pre-B cell-enhancing factor) (Visfatin)
Gene name(s) NAMPT PBEF PBEF1 Organism Nicotinamide phosphoribosyltransferase (NAmPRTase) (Nampt) (EC 2.4.2.12) (Pre-B-cell colony-enhancing factor 1) (Pre-B cell-enhancing factor) (Visfatin)
Status reviewed in uniprot database Length 491
Mass 55,521 Activity regulation Inhibited by FK866. FK866 competes for the same binding site as nicotinamide, but due to its very low dissociation rate, it is essentially an irreversible inhibitor
Involvement in disease N/A Catalytic activity Reaction=beta-nicotinamide D-ribonucleotide + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + H(+) + nicotinamide
PDB ID(s)
2E5B;2E5C;2E5D;2GVG;2GVJ;3DGR;3DHD;3DHF;3DKJ;3DKL;4JNM;4JR5;4KFN;4KFO;4KFP;4L4L;4L4M;4LTS;4LV9;4LVA;4LVB;4LVD;4LVF;4LVG;4LWW;4M6P;4M6Q;4N9B;4N9C;4N9D;4N9E;4O0Z;4O10;4O12;4O13;4O14;4O15;4O16;4O17;4O18;4O19;4O1A;4O1B;4O1C;4O1D;4O28;4WQ6;5KIT;5LX3;5LX5;5NSD;5U2M;5U2N;5UPE;5UPF;5WI0;5WI1;6ATB;6AZJ;6B75;6B76;6E68;6PEB;6TA0;6TA2;6TAC;
Function Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD.
Activity data
Interaction ID 1173
Target type Protein
Target unique ID P43490
Activity 71.7 nM
Binding Conditions/Buffer

Tris-HCl 20 mM, pH 7.4
Assay

The dose dependent saturation plots for Nampt binding to aptamer sequences G35, G40 and G55 obtained by SPR assay
PubMed ID 22143382
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_708 DNA CGTACGGAATTCGCTAGCGGGCGGGGGTGCTGGGGGAATGGAGTGCTGCGTGCTGCGGGGATCCGAGCTCCACGTG 76.32%
Apta_1115 DNA CGTACGGAATTCGCTAGCGGTGACGGACGTGGGGTGCACGAAGGGAGGGGATCCGAGCTCCACGTG 75.76%
Apta_1116 DNA CGTACGGAATTCGCTAGCCGGGGTGGGAACCAGTCTTGCGCGGGTGACGGATCCGAGCTCCACGTG 74.24%
Apta_1291 DNA CGTACGGAATTCGCTAGCCCCCCGGCAGGCCACGGCTTGGGTTGGTCCCACTGCGCGTGGATCCGAGCTCCACGTG 73.68%
Apta_910 DNA TCACGTGCATGATAGACGGCGAAGCCGTCGAGTTGCTGTGTGCCGATGCACGTGA 59.09%
Apta_1034 DNA ATGCGGATCCCGCGCGCGACCGTGTCAGCGGGGACTAGCGGTGTAGCGCGAAGCTTGCGC 57.58%
Apta_109 DNA AGCAGCACAGAGGTCAGATGTGCGACCACCTGGATAGACGAGGGTAGTAAGGAGTGTGTTCCTATGCGTGCTACCGTGA 56.96%
Apta_109 DNA AGCAGCACAGAGGTCAGATGTGCGACCACCTGGATAGACGAGGGTAGTAAGGAGTGTGTTCCTATGCGTGCTACCGTGA 56.96%
Apta_178 DNA AGCAGCACAGAGGTCAGATGCGGTATGCTGGGTCATCTAACTGGGGATATTCTGATATTGCCTATGCGTGCTACCGTGAA 56.25%
Apta_175 DNA AGCAGCACAGAGGTCAGATGAGGACTGTCGATAAGTATAATGGCAAGTAGCTCGATACATCCTATGCGTGCTACCGTGAA 55.00%
Apta_79 DNA ATCTCGAGTCATAGGGGGCGCGAACATACGCGGTTGGTGTGGTTGGCTGACTCGT 54.55%
Apta_144 DNA GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCGATAGAGGTAG 54.55%
Apta_223 DNA CATTGAGATAGCTAGTTGTAGCTGCGTCATAGGCTGGGTTGGGTCTAGTGGTTGGGTGTG 54.55%
Apta_815 DNA CATCCGTCACACCTGCTCTGGCCACTAACATGGGGACCAGGTGGTGTTGGCTCCCGTATC 54.55%
Apta_869 DNA ACTAGGACGCTTGGGAGGGGGGGTGGGGTGTCCGGTCGCG 54.55%
Apta_1219 DNA GCGCAGAACCGACAGCAAAACGGAAGTCCACCACGACTATTGGTGCCCAATCTACCGTA 54.55%
Apta_1229 DNA CACGGGCAGAGGGATAGGTTGTTGACGGGGCTGGTGGGTGGGTGCGCTCGCGCTATCGTGG 54.55%
Apta_99 DNA AGCAGCACAGAGGTCAGATGTCGGGCGGTTCGGGACCTTGCTGCGACATATGCGTATTATCCTATGCGTGCTACCGTGAA 53.75%
Apta_99 DNA AGCAGCACAGAGGTCAGATGTCGGGCGGTTCGGGACCTTGCTGCGACATATGCGTATTATCCTATGCGTGCTACCGTGAA 53.75%
Apta_174 DNA AGCAGCACAGAGGTCAGATGTCGGTCTTGTGTATACATACCCGTGTGTTTTCATCTCAGGCCTATGCGTGCTACCGTGAA 53.75%

ABOUT

AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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