AptaDB: Result

Interaction ID: 835

Aptamer sequence: GGGCACAGACGGAAGATGAGAATTGTGGGGCTTAGTATAGTGAGGTGCGTGT

Target unique ID: CCL-2

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_753	aptamer 13	Cell	Hela cells	2.5 nM	1% BSA in PBS with salts	22536456

Aptamer Sequence:	GGGCACAGACGGAAGATGAGAATTGTGGGGCTTAGTATAGTGAGGTGCGTGT
The optimal secondary structure in dot-bracket notation:	..((((..((......((((..........)))).....))...))))....
The centroid secondary structure in dot-bracket notation:	..((((..........((((..........))))..........))))....

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_753	Description	aptamer 13
Aptamer chemistry	DNA	Length	52 nt
GC content	51.9%	Molecular weight	16,348.48 Da
Molarity of 1 μg/μl solution	61.17 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Aptasensor
Sequence	GGGCACAGACGGAAGATGAGAATTGTGGGGCTTAGTATAGTGAGGTGCGTGT
Applications	Molecular probes of HPV-associated cervical cancer cells

Type	Detail	Type	Detail
ATCC ID	CCL-2	Cell name	Hela cells
Category	Human cells	Organism	Homo sapiens, human
Product type	N/A	Cell type	epithelial cell
Morphology	epithelial	Tissue	Uterus; Cervix
Disease	Adenocarcinoma	Product format	Frozen
Antigen expression	N/A	Genes expressed	Lysophosphatidylcholine (lyso-PC) induces AP-1 activity and c-jun N-terminal kinase activity (JNK1) by a protein kinase C-independent pathway; keratin positive by immunoperoxidase staining
Cell description	HeLa cells are the first immortal human cells to be grown in culture and the basis for countless significant scientific discoveries. They were isolated in 1951 from a cervical carcinoma derived from a 31-year-old patient.
Application(s)	3D cell culture

	Interaction ID	835
	Target type	Cell
	Target unique ID	CCL-2
	Activity	2.5 nM
	Binding Conditions/Buffer	1% BSA in PBS with salts
	Assay	Binding equilibrium for aptamers and their HF cell epitopes
	PubMed ID	22536456

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_180	DNA	GGTCCCCCGTAAATTGAGAATTTGGGCGTTTTGGTTCTA	57.69%
Apta_940	DNA	TGTGCGGGGGAGGGGAGTGGCGCTGTTGGTGCGGGTATAGCGCGTGGTGT	57.69%
Apta_685	DNA	GGGGCAACAAACACGGAAGAAAATGAATCTACATACGTGGACATATATCCTGCCGCGTG	57.63%
Apta_654	DNA	GCACACCGATGGCGGTCCTGTTTTACGGTAGCTTATATGCAGGGGGTGTGCGTG	57.41%
Apta_988	DNA	AAGGATGACTGACGACGATGGGAATGGAGAGGAAGGGGATATAGGTATGTTGGATGTAG	55.93%
Apta_744	DNA	GTGGCCAGTCACTCAATTGGGTGTAGGGGTGGGGATTGTGGGTTG	55.77%
Apta_756	DNA	GGGGGACACGGAGGTGGTGGAAAGGCTAAGATTTGATGATGAGTAGTGTGGT	55.77%
Apta_1200	DNA	GGCACACAGGACTATACAGTGTTGCAGTGTTGCTG	55.77%
Apta_1210	DNA	GGGGAGGCAGTGTGTTGTGTCGTGTGTGTGCTTGG	55.77%
Apta_651	DNA	CCTGCACCTCGTCCAGCATGCACACCGATGGCGGTCCTGTTTAGGGGGTGTGCGTG	55.36%
Apta_1230	DNA	GGCGATAGGCAGTGTTGCGGGGTCGGAGAGCGAGGTAATAGCGTGTATGGGTGCTGTGTG	55.00%
Apta_991	DNA	AGCAGTGAAGAGGCGAGAGATTTATGAGGGTAGCGTCCACATATGCCAGGATCGTGTTG	54.24%
Apta_140	DNA	GGCAGGAAGACAAACACGATGGGGGGGTATGATTTGATGTGGTTGTTGCATGATCGTGGTCTGTGGTGCTGT	54.17%
Apta_264	DNA	GGCGTTGTCGGGCGCAGGTGTAGGCCTCGTGGTGGTGGGT	53.85%
Apta_586	DNA	GGGAGACAAGGAAAATCCTTCAATGAAGTGGGTCGACA	53.85%
Apta_646	DNA	TGGAGCTTGGATTGATGTGGTGTGTGAGTGCGGTGCCC	53.85%
Apta_649	DNA	GCACACCGATGGCGGTCCTGTTTAGGGGGTGTGCGTG	53.85%
Apta_650	DNA	GCACACCGATGGCGGTCCTGTTTAGGGGGTGTGCGTGGGGAGGTGCGGTTGC	53.85%
Apta_751	DNA	GGTCGCAGATGGATTAAGTATGTGGGTGGGGGGGTGGAAGTTAAT	53.85%
Apta_755	DNA	GGGGAGGGAGACACAGTCATGGAGCAGTTATTAGGGTGTACCGGGTGTAGT	53.85%

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