Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 875
Aptamer sequence: ATCGTCTGCTCCGTCCAATATATCAAAGGCGAATTTTGTCAAGGTGTTAAACGATAGTCCCTACCTTTGGTGTGAGGTCGTGC
Target unique ID: CRL-2974
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_791 Aptamer TY04 Cell Multiple myeloma cells (MM.1S) 3.89 μM yeast tRNA (0.1mg/ml; Sigma) and BSA (1 mg/ml; Sigma) into the wash buffer 24792887
Structure information of aptamer
Aptamer Sequence: ATCGTCTGCTCCGTCCAATATATCAAAGGCGAATTTTGTCAAGGTGTTAAACGATAGTCCCTACCTTTGGTGTGAGGTCGTGC
The optimal secondary structure in dot-bracket notation: .......((..((.((..(((((((((((......(((((............)))))......))))))))))).)).)).))
The centroid secondary structure in dot-bracket notation: .......((..((.((..(((((((((((......(((((............)))))......))))))))))).)).)).))
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_791 Description Aptamer TY04
Aptamer chemistry DNA Length 83 nt
GC content 45.8% Molecular weight 25,587.38 Da
Molarity of 1 μg/μl solution 39.08 μM Number of G-quadruplexes No QGRS found
G-Score N/A Function Targeted therapy
Sequence ATCGTCTGCTCCGTCCAATATATCAAAGGCGAATTTTGTCAAGGTGTTAAACGATAGTCCCTACCTTTGGTGTGAGGTCGTGC
Applications Aptamer TY04 inhibits the growth of multiple myeloma cells
Target information
Type Detail Type Detail
ATCC ID CRL-2974 Cell name Multiple myeloma cells (MM.1S)
Category Human cells Organism Homo sapiens, human
Product type N/A Cell type B lymphoblast
Morphology lymphoblast Tissue Peripheral blood
Disease Immunoglobulin A Lambda Myeloma Product format Frozen
Antigen expression CD25+, CD38+, CD52+, CD59+ Genes expressed Lambda-light chain immunoglobulin
Cell description N/A
Application(s) 3D cell culture; Immune system disorder research; Immunology
Activity data
Interaction ID 875
Target type Cell
Target unique ID CRL-2974
Activity 3.89 μM
Binding Conditions/Buffer

yeast tRNA (0.1mg/ml; Sigma) and BSA (1 mg/ml; Sigma) into the wash buffer
Assay

MM.1S cells were treated with different concentrations of unselected ssDNA library (Lib) and TY04 for 96 h
PubMed ID 24792887
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_740 DNA ATCGTCTGCTCCGTCCAATAAACCTGCATAATTTATAAGTCTAGACTGCTGCATTTGGTGTGAGGTCGTGC 72.29%
Apta_743 DNA ATCGTCTGCTCCGTCCAATATCCGAGCAAGAACTCATATTGCATTATTTATAGCTACGCGCTGCATTTGGTGTGAGGTCGTGC 72.29%
Apta_736 DNA ATCGTCTGCTCCGTCCAATATGATGACACCTGCATAATTTATAGTGAGTCTTGATTCACGCTGCATTTGGTGTGAGGTCGTGC 71.08%
Apta_741 DNA ATCGTCTGCTCCGTCCAATAGCCTCACCCTGCATAATTTATAGACTACACTTAGGAATCGCTGCATTTGGTGTGAGGTCGTGC 69.88%
Apta_737 DNA ATCGTCTGCTCCGTCCAATAGTGCATTGAAACTTCTGCATCCTCGTTTGGTGTGAGGTCGTGC 67.47%
Apta_739 DNA ATCGTCTGCTCCGTCCAATAGCGTGCATTGGTTTACTGCATCCGTGAAACTGGGCTTTGGTGTGAGGTCGTGC 67.47%
Apta_738 DNA ATCGTCTGCTCCGTCCAATACCTGCATATACACTTTGCATGTGGTTTGGTGTGAGGTCGTGC 65.06%
Apta_742 DNA ATCGTCTGCTCCGTCCAATAGGACCGATAGGAACCACGGACTGCATGTTTCTGCATTTGACGTGGTTTGGTGTGAGGTCGTGC 65.06%
Apta_731 DNA AAGGAGCAGCGTGGAGGATACTCTAATTGCCGTATAAGGTCAGGGGGTTGGTTGGTTCCTAGTGCTTAGGGTGTGTCGTCGTGGT 55.29%
Apta_532 DNA ATCCGTCACACCTGCTCTAACTTACACATAATTCTAGGTTACATCTTGCTCATATGGTGTTGGCTCCCGTAT 53.01%
Apta_653 DNA CCTGCACCTCGTCCAGCATCCGATGGCGGTCCTGTTTTACGGTAGCTTATATGCAGGGGGGTG 53.01%
Apta_655 DNA CCTGCACCTCGTCCAGCATGCACACCGATGGCGGTCCTGTTTTACGGTAGCTTATATGCAGGGGGTGTGCGTG 53.01%
Apta_810 DNA ACAGCATCCCCATGTGAACAATCGCATTGTGATTGTTACGGTTTCCGCCTCATGGACGTGCTG 53.01%
Apta_1187 DNA TGTACCGTCTGAGCGATTCGTACTTTATTCGGGAAGGGTATCAGCGGGGTTCAACAAGCCAGTCAGTGTTAAGGAGTGC 53.01%
Apta_1231 DNA ACCACCGGCCCGAGATAGTCTAGACCACTATGTTGTTGTGCTTACTGCTGTGTGGTGTGG 53.01%
Apta_511 DNA ATCCGTCACACCTGCTCTCAGGGGATCACATTCTTGACGGTGTGATACAGTGCCTGGTGTTGGCTCCCGTAT 51.81%
Apta_525 DNA ATCCGTCACACCTGCTCTCAATCTGAAATATTGCAAGCAGTGCTCACAATTTGTTGGTGTTGGCTCCCGTAT 51.81%
Apta_529 DNA ATCCGTCACACCTGCTCTACACTCCGCACGATCTTTTTTAGAACGCGTACCCGTTGGTGTTGGCTCCCGTAT 51.81%
Apta_1103 DNA TACGACTCACTATAGGGATCCACCTATGGCAGATTGAGCCCAAGGGCTGTGCAGCGAATTCCCTTTAGTGAGGGTT 51.81%
Apta_1178 DNA AGCGTCGAATACCACTACAGGGGATGCGAAGTTCCGCGGTCGAGTATATGATACATCCATCTAATGGAGCTCGTGGTCAG 51.81%

ABOUT

AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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