AptaDB: Result

Interaction ID: 642

Aptamer sequence: TCGCGCGAGTCGTCTGGGGGACTAGAGGACTTGTGCGGCCCCGCATCGTCCTCCC

Target unique ID: 25922

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_509	Antibac2	Cell	Escherichia Coli (E.Coli)	62.43 nM	PBS containing 1.4 mM MgCl2 and 0.05% BSA, Room temperature (45 mins)	28119514

Aptamer Sequence:	TCGCGCGAGTCGTCTGGGGGACTAGAGGACTTGTGCGGCCCCGCATCGTCCTCCC
The optimal secondary structure in dot-bracket notation:	.((((((((((.(((((....))))).))))))))))..................
The centroid secondary structure in dot-bracket notation:	.((((((((((.(((((....))))).))))))))))..................

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_509	Description	Antibac2
Aptamer chemistry	DNA	Length	55 nt
GC content	69.1%	Molecular weight	16,912.77 Da
Molarity of 1 μg/μl solution	59.13 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Aptasensor
Sequence	TCGCGCGAGTCGTCTGGGGGACTAGAGGACTTGTGCGGCCCCGCATCGTCCTCCC
Applications	Rapid bacteria detection

Type	Detail	Type	Detail
ATCC ID	25922	Cell name	Escherichia Coli (E.Coli)
Category	Bacteria	Organism	N/A
Product type	Preceptrol culture	Cell type	N/A
Morphology	N/A	Tissue	N/A
Disease	N/A	Product format	Freeze-dried
Antigen expression	N/A	Genes expressed	N/A
Cell description	This strain does not produce verotoxin. This organism is a CLSI control strain for antimicrobial susceptibility testing. Used in susceptibility disc testing of neomycin, colistin [colimycin], kanamycin, cephalexin, gentamicins, cefamandole, cephalothin, tetracycline, cephaloglycin, cephaloridine [cephalomycin], nalidixic acid, and chloramphenicol.
Application(s)	Bioinformatics; Food testing; Media testing; Quality control

	Interaction ID	524
	Target type	Cell
	Target unique ID	35595
	Activity	195.90 nM
	Binding Conditions/Buffer	PBS containing 1.4 mM MgCl2 and 0.05% BSA; Room temperature (45 mins)
	Assay	Binding affinity of Antibac2 aptamer
	PubMed ID	28119514
	Interaction ID	525
	Target type	Cell
	Target unique ID	BAA-2313
	Activity	194.90 nM
	Binding Conditions/Buffer	PBS containing 1.4 mM MgCl2 and 0.05% BSA; Room temperature (45 mins)
	Assay	Binding affinity of Antibac2 aptamer
	PubMed ID	28119514
	Interaction ID	528
	Target type	Cell
	Target unique ID	19115
	Activity	54.19 nM
	Binding Conditions/Buffer	PBS containing 1.4 mM MgCl2 and 0.05% BSA; Room temperature (45 mins)
	Assay	Binding affinity of Antibac2 aptamer
	PubMed ID	28119514
	Interaction ID	532
	Target type	Cell
	Target unique ID	BAA-1605
	Activity	71.92 nM
	Binding Conditions/Buffer	PBS containing 1.4 mM MgCl2 and 0.05% BSA; Room temperature (45 mins)
	Assay	Binding affinity of Antibac2 aptamer
	PubMed ID	28119514
	Interaction ID	642
	Target type	Cell
	Target unique ID	25922
	Activity	62.43 nM
	Binding Conditions/Buffer	PBS containing 1.4 mM MgCl2 and 0.05% BSA, Room temperature (45 mins)
	Assay	Binding affinity of Antibac2 aptamer
	PubMed ID	28119514

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_1125	DNA	TCGGGCGAGTCGTCTGGGGAGGTCAGAACGAAAGGCCCGCATCGTCCTCCC	76.36%
Apta_1126	DNA	TCGGGCGAGTCGTCTGCGGGCGGATAGACTACGACACCGCATCGTCCTCCC	76.36%
Apta_1127	DNA	TCGGGCGAGTCGTCTGCGAGGCAACAAGCTCGTAATCCGCATCGTCCTCCC	72.73%
Apta_558	DNA	CCGGTGGGTGGTCAGGTGGGATAGCGTTCCGCGTATGGCCCAGCGCATCACGGGTTCGCACCA	58.73%
Apta_369	DNA	GGCTGTTGTGAGCCTCCTCCCAGAGGGAAGACTTTAGGTTCGGTTCACGTCCCGCTTATTCTTACTCCC	56.52%
Apta_255	DNA	TAGCGATACTGCGTGGGTTGGGGCGGGTAGGGCCAGCAGTCTCGT	56.36%
Apta_556	DNA	GGCTCTCGGGACGACATGGATTTTCCATCAACGAAGTGCGTCCGTCCC	56.36%
Apta_488	DNA	CGACCAGGGTGGGAGGGGGACGGGCTGGCGGCGCGCGGTAATG	54.55%
Apta_1014	DNA	TGGGGGTTGGGTGGATAGGCTGGCGTCGGGGCAGGTCAGT	54.55%
Apta_228	DNA	AGCAGCACAGAGGTCAGATGCGGCGCGGGGTCTTTTACAAATAGTTGTCCTAGCCACGCCTATGCGTGCTACCGTGAA	53.85%
Apta_311	DNA	GTGGGGTTGGGTGGGTTTGGGTTATGGGCGCTCCGGCCCGGCGTCTTGCGTCCCTTCCAT	53.33%
Apta_878	DNA	TCGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCTGTGGGTGGGCTTCGCACACACGGACTTAC	53.16%
Apta_1116	DNA	CGTACGGAATTCGCTAGCCGGGGTGGGAACCAGTCTTGCGCGGGTGACGGATCCGAGCTCCACGTG	53.03%
Apta_1117	DNA	CGTACGGAATTCGCTAGCGGACTGGAGTCTAGACCGGGTAGCTGTGGTGGATCCGAGCTCCACGTG	53.03%
Apta_341	DNA	TCGGGGTTTGGGGGTTCGGGGTCGGTTCGGTTTCTTCTAATGGA	52.73%
Apta_592	DNA	CTCTCGGGACGACCACTACGCATAGTTTCTATCGCCAGGAAGGGTCGTCCC	52.73%
Apta_593	DNA	CTCTCGGGACGACAGCCGAGTTGATTCAACAGCCGAGTCGTCCC	52.73%
Apta_636	DNA	TAGCCGGATCGCGCTGGCCAGATGATATAAAGGGTCAGCCCCCCA	52.73%
Apta_697	DNA	CTCTCGGGACGACGGACGCTAATCTTACAAGGGCGTAGTGTATGTCGTCCC	52.73%
Apta_857	DNA	GCTATGGGTGGTCTGGTTGGGATTGGCCCCGGGAGCTGGC	52.73%

ABOUT