Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 313
Aptamer sequence: CGGCCGAUAGAAAGACUACUUGAGCCCUUAAAUGAGGUUAUGUGCGGCCG
Target unique ID: P13562
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_304 | A10 | Protein | GON1_MOUSE | 190 nM | 10 mM HEPES (pH 7.5), 137 mM NaCl, 2.7 mM KCl, 2 mM CaCl2, 1 mM MgCl2, 0.005% Triton X-100 | 11927260 |
Structure information of aptamer
| Aptamer Sequence: | CGGCCGAUAGAAAGACUACUUGAGCCCUUAAAUGAGGUUAUGUGCGGCCG |
| The optimal secondary structure in dot-bracket notation: | ((((((..........(((.(((..((((....))))))).))))))))) |
| The centroid secondary structure in dot-bracket notation: | ((((((...................((((....)))).......)))))) |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_304 | Description | A10 |
| Aptamer chemistry | RNA | Length | 50 nt |
| GC content | 52.0% | Molecular weight | 16,360.56 Da |
| Molarity of 1 μg/μl solution | 61.12 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted binding |
| Sequence | CGGCCGAUAGAAAGACUACUUGAGCCCUUAAAUGAGGUUAUGUGCGGCCG | ||
| Applications | Binding to GnRH | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P13562 | Protein name | Progonadoliberin-1 (Progonadoliberin I) [Cleaved into: Gonadoliberin-1 (Gonadoliberin I) (Gonadotropin-releasing hormone I) (GnRH-I) (Luliberin I) (Luteinizing hormone-releasing hormone I) (LH-RH I); Prolactin release-inhibiting factor 1 (Prolactin release-inhibiting factor I)] |
| Gene name(s) | Gnrh1 Gnrh | Organism | Progonadoliberin-1 (Progonadoliberin I) [Cleaved into: Gonadoliberin-1 (Gonadoliberin I) (Gonadotropin-releasing hormone I) (GnRH-I) (Luliberin I) (Luteinizing hormone-releasing hormone I) (LH-RH I); Prolactin release-inhibiting factor 1 (Prolactin release-inhibiting factor I)] |
| Status | reviewed in uniprot database | Length | 90 |
| Mass | 10,337 | Activity regulation | N/A |
| Involvement in disease | N/A | Catalytic activity | N/A |
| PDB ID(s) | N/A |
 |
Function | Stimulates the secretion of gonadotropins. |
Activity data
|
No relevant experimental diagram |
Interaction ID | 313 |
| Target type | Protein | |
| Target unique ID | P13562 | |
| Activity | 190 nM | |
| Binding Conditions/Buffer | 10 mM HEPES (pH 7.5), 137 mM NaCl, 2.7 mM KCl, 2 mM CaCl2, 1 mM MgCl2, 0.005% Triton X-100 |
|
| Assay | N/A |
|
| PubMed ID | 11927260 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_703 | RNA | GGGCGGAUGAGACGCUUGGCGUGUGCUGUGGAGAGUCAUCCG | |
| Apta_367 | RNA | CGAGCGUAGAAUGUCUUCCCACUAGACGUCGUUCGGCCCC | |
| Apta_426 | RNA | UGCCGCUAUAAUGCACGGAUUUAAUCGCCGUAGAAAAGCAUGUCAAAGCCG | |
| Apta_370 | RNA | GGGAGGACGCGUCGCCGUAAUGGAUGUUUUGCUCCCUG | |
| Apta_1167 | RNA | GGCGCGAGGAAGGAGGUCUGAGGAGGUCACUGCGCC | |
| Apta_38 | RNA | GGUAGAUACGAUGGAUACCCCCUGUGGCCCGUCAACACAGGGGAAGUGGCAUGACGCGCAGCCA | |
| Apta_230 | RNA | GGGAGGACGAUGCGGGCGUGAAUGGUGUUGUGAGG | |
| Apta_293 | RNA | CGGCCACAGAAUGAAAAACCUCAUCGAUGUUGCAUAGUUG | |
| Apta_632 | RNA | GACGAGAAGGAGUGCUGGUUAUACUAGCGGUUAGGUCACUCGUC | |
| Apta_849 | RNA | UCCCGAUUACUGUACAUACCUUAGCCCAUAGCUGGCUGC | |
| Apta_171 | RNA | GGGAGCUCAGAAUAAACGCUCAACCAGUCUUGUGGCUUUGAAAGAGAGGAGUGUUCGACAUGAGGCCCGGAUCCGGC | |
| Apta_1069 | DNA | CCGGAGAAAAAAAACTTGATGAGCCAGGAAAACGAGGGTTGGAACGCGACTG | |
| Apta_39 | RNA | GGUAGAUACGAUGGACUACCGCCACCCCGGGUCCGGGUGACGUUAAUUGAGGGCCCAUGACGCGCAGCCA | |
| Apta_425 | RNA | UGCCGCCAUAUCACACGGAUUUAAUCGCCGUAGAAAAGCAUGUCAAAGCCG | |
| Apta_696 | RNA | GAGUGCGCCCAUUGAUAAGAGCGUAGGUUCAACGGAGUCGUAGUCGUUCUCAAGUUGACGGUGCAUUAGAGUCCU | |
| Apta_257 | DNA | GGCAGAAGAAATATCGAAACCCAGAATGGTCGGCCAGGCG | |
| Apta_431 | RNA | CAGGCAUGCCUAGCUAAGCAGCCCAUGGCUUAUGCGCGGAAUAUUGGCUUCCGUUC | |
| Apta_521 | RNA | GGGUUGGGAAGAAACUGUGGCACUUCGGUGCCAGCAACCC | |
| Apta_560 | RNA | GGAAGAGAUGGCGACUAAAACGACUUGUCGC | |
| Apta_603 | RNA | CUUGUCUGAACAAUUUCGUCUGCCUUUGCGUUCCGACGGC |
DEV TOOL
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