AptaDB: Result

Interaction ID: 425

Aptamer sequence: ATCAGGGCTAAAGAGTGCAGAGTTACTTAG

Target unique ID: P83673

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_419	clone1	Protein	LYS1_CRAVI	31 nM	20 mM Tris (pH 7.5) 100 mM NaCl, 5 mM MgCl2	11557339

Aptamer Sequence:	ATCAGGGCTAAAGAGTGCAGAGTTACTTAG
The optimal secondary structure in dot-bracket notation:	............(((((......)))))..
The centroid secondary structure in dot-bracket notation:	............(((((......)))))..

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_419	Description	clone1
Aptamer chemistry	DNA	Length	30 nt
GC content	43.3%	Molecular weight	9,319.04 Da
Molarity of 1 μg/μl solution	107.31 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted binding
Sequence	ATCAGGGCTAAAGAGTGCAGAGTTACTTAG
Applications	Binding to target proteins

Type	Detail	Type	Detail
Uniprot ID	P83673	Protein name	Lysozyme 1 (EC 3.2.1.17) (1,4-beta-N-acetylmuramidase 1) (Invertebrate-type lysozyme 1) (cv-lysozyme 1)
Gene name(s)	lysoz1 lysoz	Organism	Lysozyme 1 (EC 3.2.1.17) (1,4-beta-N-acetylmuramidase 1) (Invertebrate-type lysozyme 1) (cv-lysozyme 1)
Status	reviewed in uniprot database	Length	184
Mass	19,986	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	Reaction=Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
PDB ID(s)	N/A
Function	Has antibacterial activity against the Gram-positive bacteria L.garvieae, M.luteus and Enterococcus sp.

	Interaction ID	425
	Target type	Protein
	Target unique ID	P83673
	Activity	31 nM
	Binding Conditions/Buffer	20 mM Tris (pH 7.5) 100 mM NaCl, 5 mM MgCl2
	Assay	The binding activities for the selected aptamers are shown. Light green bars illustrate percent binding in the absence of protein, while dark green bars show percent binding in the presence of 500 nM lysozyme
	PubMed ID	11557339

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_421	DNA	ATCTACGAATTCATCAGGGCTAAAGAGTGCAGAGTTACTTAGCCCGTGA	61.22%
Apta_1200	DNA	GGCACACAGGACTATACAGTGTTGCAGTGTTGCTG	60.00%
Apta_185	DNA	ACCAAGGCTTTAGGTCTGAGATCTCCCAAT	56.67%
Apta_196	DNA	AGGAAGGCTTTAGGTCTGAGATCTC	56.67%
Apta_203	DNA	GGAAGGCTTTAGGTCTGAGATCTCGG	56.67%
Apta_204	DNA	CCAAGGCTTTAGGTCTGAGATCTCCC	56.67%
Apta_205	DNA	TTAAGGCTTTAGGTCTGAGATCTCTT	56.67%
Apta_343	DNA	AACAGAGGGACAAACGGGGGAAGATTTGACGTCGACGAC	56.41%
Apta_438	DNA	AGCCATGACGATGTCGTTACGTAGATGCAGAGACTCCTAA	55.00%
Apta_438	DNA	AGCCATGACGATGTCGTTACGTAGATGCAGAGACTCCTAA	55.00%
Apta_579	DNA	ACTGAGGGCACGGACAGGAGGGGGAGAGATGGCGTGAGGT	55.00%
Apta_1139	DNA	AATAGGGTAAAAAAAAAAAGTTGGTCCTATG	54.84%
Apta_291	DNA	AAAAATCTAGGCGCTGAAGCCCCGCTTCCTTACCTTCAC	53.85%
Apta_1032	DNA	TGGCAAGAGGGTGTGCTTAAGGTGGACACGGTGGCTTAG	53.85%
Apta_345	DNA	ACAGTAACCGCGTGAGGTCGA	53.33%
Apta_546	DNA	CACGGCATGGTGGGCGTCGTG	53.33%
Apta_630	DNA	GACCAGGGCAAACGGTAGGGAGTGGTC	53.33%
Apta_1150	DNA	CTAAGCAGGAAGGTATGGATTGGTTGCATTAG	53.12%
Apta_443	DNA	GGCCATGACGATGTCGTTACGTAGATGCAGAGACTCCTAA	52.50%
Apta_444	DNA	AGCCATTACGATGTCGTTACGTAGATGCAGAGACTCCTAA	52.50%

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