Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 849
Aptamer sequence: GGUUACCAGCCUUCACUGCGGACGGACAGAGAGUGCAACCUGCCGUGCCGCACCACGGUCGGUCACAC
Target unique ID: HTB-14
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_766 aptamer H02 Cell Cells expressing integrin a5b1 72 nM 1 mM MgCl2 and 0.5 mM CaCl2 in PBS (pH 7.4) 31226519
Structure information of aptamer
Aptamer Sequence: GGUUACCAGCCUUCACUGCGGACGGACAGAGAGUGCAACCUGCCGUGCCGCACCACGGUCGGUCACAC
The optimal secondary structure in dot-bracket notation: .((.(((.(((.....(((((((((.(((..........))))))).)))))....))).))).))..
The centroid secondary structure in dot-bracket notation: .((.(((.(((.....(((((((((.(((..........))))))).)))))....))).))).))..
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_766 Description aptamer H02
Aptamer chemistry RNA Length 68 nt
GC content 64.7% Molecular weight 21,837.04 Da
Molarity of 1 μg/μl solution 45.79 μM Number of G-quadruplexes No QGRS found
G-Score N/A Function Aptasensor
Sequence GGUUACCAGCCUUCACUGCGGACGGACAGAGAGUGCAACCUGCCGUGCCGCACCACGGUCGGUCACAC
Applications Probe for cyto-and histofluorescence in glioblastoma
Target information
Type Detail Type Detail
ATCC ID HTB-14 Cell name Cells expressing integrin a5b1
Category Human cells Organism Homo sapiens, human
Product type N/A Cell type N/A
Morphology epithelial Tissue Brain
Disease Likely Glioblastoma Product format Frozen
Antigen expression N/A Genes expressed N/A
Cell description U-87 MG is a cell line with epithelial morphology that was isolated from malignant gliomas from a male patient, likely with Glioblastoma. Use these cells in your neuroscience and immuno-oncology research.
Application(s) 3D cell culture; Neuroscience; Immuno-oncology
Activity data
Interaction ID 849
Target type Cell
Target unique ID HTB-14
Activity 72 nM
Binding Conditions/Buffer

1 mM MgCl2 and 0.5 mM CaCl2 in PBS (pH 7.4)
Assay

KD for the interaction between U87MG a5+ cells and aptamers H02
PubMed ID 31226519
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_314 RNA GGUUACCAGCCUUCACUGCUCCCGGACGCGUCUGUAUCCGCUACCCGCCGCACCACGGUCGGUCACAC 79.41%
Apta_936 RNA GGAGCUCAGCCUUCACUGCGAUCACCGUGUCGGUGGCUAGUCGAUGUAUCCCGAUCACCAUUGGGGGCAAGUUUAGACAGGCACCACGGUCGGAUCCAC 56.57%
Apta_561 RNA GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGCCUGUGGAAACAGACGUGGCACAUGACUACGUCGAAACGGUAGCGAGAGCUC 55.56%
Apta_710 RNA GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGUCUGGAUACCAUGCAUGAUGCACCUUGGCAGUCUUACGAAACGGUAGCGAGAGCUC 55.32%
Apta_566 RNA GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGCCCUGCGAUGCAGAAAGGUGCUGACGACACAUCGAAACGGUAGCGAGAGCUC 54.44%
Apta_816 RNA GGGUUCACUGCAGACUUGACGAAGCUUGAGAGAUGCCCCCUGAUGUGCAUUCUUGUUGUGUUGCGGCAAUGGAUCCACAUCUACGAAUUC 54.44%
Apta_563 RNA GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGAAAGCCAAAGCCGUAGCGCAGAUGAUCUCGCCAUCAGUACCGAAACGGUAGCGAGAGCUC 54.08%
Apta_549 RNA GGAGCUCAGCCUUCACUGCAUGAUAAACCGAUGCUGGGCGAUUCUCCUGAAGUAGGGGAAGAGUUGUCAUGUAUGGGGGCACCACGGUCGGAUCCUG 53.61%
Apta_292 RNA CUACGAACUCAUGACACAAGGAUGCAAUCUCAUCCCGCCAGCCCACCGU 52.94%
Apta_613 RNA GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGCCUUAGGAUAUGCAUGAUGCAGAAGGACGUCGAAACGGUAGCGAGAGCUC 52.27%
Apta_157 RNA GGGUUCACUGCAGACUUGACGAAGCUUACAAACAAGAGCAAAAAGGGAGUUGACGUAGACUGUGCGGAAUGGAUCCACAUCUACGAAUUC 52.22%
Apta_562 RNA GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGCCUUUAGGGCCAAGUGUGGUGAAAGACACACUCGAAACGGUAGCGAGAGCUC 52.22%
Apta_376 RNA GGGAGCUCAGCCUUCACUGCACUCCGGCUGGUGGACGCGGUACGAGCAAUUUGUACCGGAUGGAUGUUCGGGCAGCGGUGUGGCAGGGAUGAGCGGCACCACGGUCGGAUCCAC 51.75%
Apta_413 RNA GUGAGACCAGCCGAGUGGUGUCUGGCUAUUCACUGGAGCGUGGGUGGAACCCCUGCGCACUCGUUUGGCUGUCCGGGCCUUCGGGCCGGGAUUAUCUCU 51.52%
Apta_155 RNA GACCCAGGGCCGGGACGCAAAGCAGCCAAAACAGACGGCCCCAGUCAGGGAGUU 51.47%
Apta_447 RNA ACGCUCAACGAGCCAGGAACAUCGACGUCAGCAAACGCGAGCGCAACCAGUAACACC 51.47%
Apta_305 RNA GGGAGGACGAUGCGGAUCGCCCUGAACCGGCCCAGCAGACUGCUGACGGCACGAUCCGCAUCGUCCUCCC 51.43%
Apta_294 RNA AUACCAGCUUAUUCAAUUGCCUGAUUAGCGGUAUCACGAUUACUUACCUUCGUUGCUGAGAUAGUAAGUGCAAUCU 51.32%
Apta_1054 RNA CUGACGUUUCGAACGUCAAAUGCGGAAGUUGUCGGAGCUAAGAAACGGGUAAUGAUGCCGUUGAUGUGAAGGCGGGCUCA 51.25%
Apta_137 RNA GGGAGACAAGAAUAAACGCUCAAGUGACGUCCUGAUCGAUUGUGCAUUCGGUGUGACGAUCUUUCGACAGGAGGCUCACAACAGGC 51.16%

ABOUT

AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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