AptaDB: Result

Interaction ID: 293

Aptamer sequence: CACTCCGTCACTTTAGTGGATCTTTATAAAACCGATGCTG

Target unique ID: P0DOF8

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_284	HA7	Protein	M2_I72A8	22.3 nM	50 mM NaH2PO4, 50 mM NaCl, and 20 mM imidazole (pH 8.0) for 1 hour	23077124

Aptamer Sequence:	CACTCCGTCACTTTAGTGGATCTTTATAAAACCGATGCTG
The optimal secondary structure in dot-bracket notation:	.......((((....)))).....................
The centroid secondary structure in dot-bracket notation:	........................................

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_284	Description	HA7
Aptamer chemistry	DNA	Length	40 nt
GC content	42.5%	Molecular weight	12,460.82 Da
Molarity of 1 μg/μl solution	80.25 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Target detection
Sequence	CACTCCGTCACTTTAGTGGATCTTTATAAAACCGATGCTG
Applications	Be used in rapid detection and typing protocols for fifield applications

Type	Detail	Type	Detail
Uniprot ID	P0DOF8	Protein name	Matrix protein 2 (Proton channel protein M2)
Gene name(s)	M	Organism	Matrix protein 2 (Proton channel protein M2)
Status	reviewed in uniprot database	Length	97
Mass	11,186	Activity regulation	The M2 protein from most influenza A strains is inhibited by amantadine and rimantadine, resulting in viral uncoating incapacity
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	N/A
Function	Forms a proton-selective ion channel that is necessary for the efficient release of the viral genome during virus entry.

	Interaction ID	293
	Target type	Protein
	Target unique ID	P0DOF8
	Activity	22.3 nM
	Binding Conditions/Buffer	50 mM NaH2PO4, 50 mM NaCl, and 20 mM imidazole (pH 8.0) for 1 hour
	Assay	The dissociation constant (Kd) of each DNA aptamer was used to describe the strength of the binding affinity between aptamer and recombinant swH3 protein. The Kd was calculated from the dot blot chemiluminescence intensity based on a nonlinear regression equation
	PubMed ID	23077124

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_285	DNA	GCCACTCCGTCACTTTAGTGGTTTTTTTTATAAATGCTTC	72.50%
Apta_286	DNA	TACTCCGTCACTTTAGTGGATATAACAACCTCCCTATGGG	70.00%
Apta_283	DNA	ACTCCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGA	55.00%
Apta_882	DNA	AATAGTCTGGTTCGGTGCTTGGTGCGATGTGTTAGTCAGACTCTTGTGTG	54.00%
Apta_75	DNA	CACTGGTAGGTTGGTGTGGTTGGGGCCAGTG	52.50%
Apta_158	DNA	CACAGGCTACGGCACGTAGAGCATCACCATGATCCTGTG	52.50%
Apta_167	DNA	TGCTCCTTGGCCTTAGCCCTGGAACCATCAATCCTCTTCG	52.50%
Apta_227	DNA	ACGCGCCTATGGTGCATCGCATGCCGACC	52.50%
Apta_691	DNA	CGACGCGCGTTGGTGGTGGATGGTGTGTTACACGTGTTGT	52.50%
Apta_822	DNA	TAGCCCTTCAACATAGTAATATCTCTGCATTCTGTGATG	52.50%
Apta_1203	DNA	CCCTGCGGGGCTACCCGATATGTGTCCGAGTGGTG	52.50%
Apta_593	DNA	CTCTCGGGACGACAGCCGAGTTGATTCAACAGCCGAGTCGTCCC	52.27%
Apta_654	DNA	GCACACCGATGGCGGTCCTGTTTTACGGTAGCTTATATGCAGGGGGTGTGCGTG	51.85%
Apta_179	DNA	TCGGTTGAGTGAGATTAGTCAAATGTTTAGAAGACAACCTC	51.22%
Apta_1023	DNA	CTCCTTCAACGAAGTGGGTCTCCTTCAACGAAGTGGGTCTC	51.22%
Apta_640	DNA	CAATGGACCTATTCGAGTACTGAATAGAACAGTCGGCGCTCTGGG	51.11%
Apta_845	DNA	CCAAACGCGCTGGTTTGGAATGCTTAGAGTTTGATCGATGCCTAA	51.11%
Apta_987	DNA	GTACTGTCAATTGGAAGTGGTGTTACGTTGTGTAGTCAAATCAGTGC	51.06%
Apta_1033	DNA	CACAGGTTCCAGGTAATACCTAAGGGTATGCTCTCGCCTATTATATGGAGCAC	50.94%
Apta_621	DNA	AAGCTTGCTTTATAGCCTGCAGCGATTCTTGATCGGAAAAGGCTGAGAGCTACGC	50.91%

ABOUT