AptaDB: Result

Interaction ID: 1014

Aptamer sequence: GCCUUUUCGGGAUACGCGGAGGGUGGGCAA

Target unique ID: Other-34

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_924	TAFapt1	Other	leukemic AML1-MTG8 fusion protein	3.61 nM	20 mM sodium phosphate (pH 6.5), 2 mM magnesium acetate, 200 mM potassium acetate, 5% glycerol, 0.05% Triton X-100, 5 mM b-mercaptoethanol	32870501

Aptamer Sequence:	GCCUUUUCGGGAUACGCGGAGGGUGGGCAA
The optimal secondary structure in dot-bracket notation:	(((((((((.......)))))...))))..
The centroid secondary structure in dot-bracket notation:	(((((((((.......)))))...))))..

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_924	Description	TAFapt1
Aptamer chemistry	RNA	Length	30 nt
GC content	63.3%	Molecular weight	9,739.82 Da
Molarity of 1 μg/μl solution	102.67 μM	Number of G-quadruplexes	2
G-Score	18	Function	Targeted therapy
Sequence	GCCUUUUCGGGAUACGCGGAGGGUGGGCAA
Applications	The aptamer could bind to the AML1-MTG8 fusion protein and dissociate the AML1-MTG8/DNA complex, suggesting that it can inhibit the dominant negative effects of AML1-MTG8 against normal AML1 function and serve as a potential therapeutic agent for leukemia

Type	Detail	Type	Detail
Target ID	Other-34	Name	leukemic AML1-MTG8 fusion protein

No relevant experimental diagram	Interaction ID	1014
	Target type	Other
	Target unique ID	Other-34
	Activity	3.61 nM
	Binding Conditions/Buffer	20 mM sodium phosphate (pH 6.5), 2 mM magnesium acetate, 200 mM potassium acetate, 5% glycerol, 0.05% Triton X-100, 5 mM b-mercaptoethanol
	Assay	N/A
	PubMed ID	32870501

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_377	RNA	CUUCGGGAUUUGAGGGUAGAAUGGGACUA	56.67%
Apta_319	RNA	ACCCUGAUGGUAGACGCCGGGGUG	53.33%
Apta_855	RNA	UCACUGUUAUCCGAUAGCAGCGCGGGAUGA	53.33%
Apta_243	RNA	GCUUGAAGCUAGCAGUAAGACUAGCGCUACGGAUGGGUGUGCG	51.16%
Apta_218	RNA	AGCCGCGAGGGGAGGGAUAGGGUAGGGCGCGGCU	50.00%
Apta_231	RNA	GGGAGGACGAUGCGGGUGUGGGGCG	50.00%
Apta_630	DNA	GACCAGGGCAAACGGTAGGGAGTGGTC	50.00%
Apta_488	DNA	CGACCAGGGTGGGAGGGGGACGGGCTGGCGGCGCGCGGTAATG	48.84%
Apta_47	DNA	GACCTAGCAGTGGACATGTGGCAGGGTGAAGTGGCATCGTC	48.78%
Apta_402	RNA	GCCUGUAAGGUGGUCGGUGUGGCGAGUGUGUUAGGAGAGAUUGC	47.73%
Apta_365	RNA	GGACCUAUGCAGUAGCCAGUGUGGACUGGGCUGCCCCCCC	47.50%
Apta_521	RNA	GGGUUGGGAAGAAACUGUGGCACUUCGGUGCCAGCAACCC	47.50%
Apta_831	DNA	CCGCCCAGCGGGGGTAGGGCCGGACGTAGGAGGAGCTGCG	47.50%
Apta_880	DNA	GCCGCACAATCGGGTTGGGCAGGGAGGGCACGTGTGGCGC	47.50%
Apta_1190	DNA	TTCAGGGCGGGGTAAACGGGAGGTGGGGGGGGCTTGGGAC	47.50%
Apta_848	RNA	CACCACGAUCACGGUUUCCCUCGCAGGUAAGGUGUAGA	47.37%
Apta_1142	RNA	ACGAGUCACACGUUGAUGACUGGAUGGUAGUUAAAGAGGGUGGGGCAACAA	47.06%
Apta_634	RNA	GGUCUUACGUCGUUCGCGACUAUUGGGAGACC	46.88%
Apta_381	RNA	AUUCUGUUGGCGAACUGUACGCAAGUACUGGAUGACAGCCUAUCUAU	46.81%
Apta_27	DNA	AGGGGGAGGGGTGGGGCCGGGCAGA	46.67%

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