Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 831
Aptamer sequence: GATCGGTGGGTGGGGGGGTTGGAGATCATCCTCAGGGATTACGTC
Target unique ID: CRM-CCL-185
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_749 | S11.d | Cell | vaccinia virus–infected and–uninfected lung cancer A549 cells | 60.1 nM | 5 mmol MgCl2 and 4.5 g glucose into 1 L of Dulbecco's PBS without calcium and magnesium | 19684903 |
Structure information of aptamer
| Aptamer Sequence: | GATCGGTGGGTGGGGGGGTTGGAGATCATCCTCAGGGATTACGTC |
| The optimal secondary structure in dot-bracket notation: | ((((......((((((((((...)))).))))))..))))..... |
| The centroid secondary structure in dot-bracket notation: | ..........((((((((((...)))).))))))........... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_749 | Description | S11.d |
| Aptamer chemistry | DNA | Length | 45 nt |
| GC content | 60.0% | Molecular weight | 14,085.00 Da |
| Molarity of 1 μg/μl solution | 71.00 μM | Number of G-quadruplexes | 3 |
| G-Score | 54 | Function | Target recognition |
| Sequence | GATCGGTGGGTGGGGGGGTTGGAGATCATCCTCAGGGATTACGTC | ||
| Applications | Recognition of subtype non-small cell lung cancer | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| ATCC ID | CRM-CCL-185 | Cell name | vaccinia virus-infected and-uninfected lung cancer A549 cells |
| Category | Human cells | Organism | Homo sapiens, human |
| Product type | Certified reference material | Cell type | epithelial cell |
| Morphology | epithelial | Tissue | Lung |
| Disease | Carcinoma | Product format | Frozen |
| Antigen expression | N/A | Genes expressed | N/A |
| Cell description | N/A |
|
|
| Application(s) | Assay development | ||
Activity data
|
No relevant experimental diagram |
Interaction ID | 831 |
| Target type | Cell | |
| Target unique ID | CRM-CCL-185 | |
| Activity | 60.1 nM | |
| Binding Conditions/Buffer | 5 mmol MgCl2 and 4.5 g glucose into 1 L of Dulbecco's PBS without calcium and magnesium |
|
| Assay | N/A |
|
| PubMed ID | 19684903 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_607 | DNA | TGAGGCGGGTGGGTGGGTTGAATATGCTGATTACCCCATCGGAGAACGTTAAGGCGCTTC | |
| Apta_857 | DNA | GCTATGGGTGGTCTGGTTGGGATTGGCCCCGGGAGCTGGC | |
| Apta_124 | DNA | GGTGGTGGGGGGGGTTGGTAGGGTGTCTTC | |
| Apta_565 | DNA | GCGGGGTTGGGCGGGTGGGTTCGCTGGGCAGGGGGCGAGTG | |
| Apta_959 | DNA | GGACCGCTAGGTTCGTGGCTGAGATCACGCGATATCAGTA | |
| Apta_608 | DNA | TGAGGCGGGTGGGTGGGTTGAATACGCTGATCACCCCATCGGAGAACGTTAAGGCGCTTT | |
| Apta_754 | DNA | GGGCGGGGAGTAGGGAGAGGGGTTTCCATCGGCGACAGAGGAGTTATGTGTGT | |
| Apta_50 | DNA | AGTGGCGAGGGGGGTAGGTAAAGTATCCTTAGTGTGTTG | |
| Apta_148 | DNA | CCGTAGGTTCGGGGTCGGAGTGGTCCGGAAGGTGGCGTGG | |
| Apta_149 | DNA | CCGTAGGTTCGGGGTCGGAGTGGTCCGGAAGATGGCGTGG | |
| Apta_746 | DNA | AGAGTGGGGGGGTGGGTGGATTTGACAGGTGGCATGCTGGAGAGT | |
| Apta_850 | DNA | CACCAGTGTGTTGAGGTTTGAGCACACTGATAGAGTGTCA | |
| Apta_1188 | DNA | CGGGCGGGGCGTGGGGTGTTGGAGTGGAGGGCGGGGCGGC | |
| Apta_1256 | DNA | GATCGGGTGTGGGTGGCGTAAAGGGAGCATCGGACA | |
| Apta_609 | DNA | GCTGCGAGGTGGGTGGGTGGGAGCAATTGGTCCTCGCTTAGCTTCTACGGTGGGCTATCT | |
| Apta_611 | DNA | GCTGCGAGGTGGGTGGGTGGGAGCAATTGGTCCTTGCTTAGCTTCTACGGTGGGCTATCT | |
| Apta_616 | DNA | GGTGCGGGTAGGGATGAGGGCGGGTCGGGTTGAGTGTGAGCTTAGGGGtGGCGGATCTAC | |
| Apta_28 | DNA | AGTGGGGGGGGTGGAGCCGGGTAGA | |
| Apta_145 | DNA | GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCGATAGA | |
| Apta_146 | DNA | GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCG |
DEV TOOL
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