Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 862
Aptamer sequence: GAAGACGAGCGGCGAGTGTTATTACGCTTGGAAACAACCCC
Target unique ID: CRL-5800
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_778 | EJ4 | Cell | Lung Adenocarcinoma (H23) | 60 nM | 0.45% w/v glucose 5 mM MgCl2 in PBS 1 mg/mL tRNA 1 mg/mL BSA incubate with cells for 30 minutes | 23082117 |
Structure information of aptamer
| Aptamer Sequence: | GAAGACGAGCGGCGAGTGTTATTACGCTTGGAAACAACCCC |
| The optimal secondary structure in dot-bracket notation: | .....((((((.............))))))........... |
| The centroid secondary structure in dot-bracket notation: | .....((((((.............))))))........... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_778 | Description | EJ4 |
| Aptamer chemistry | DNA | Length | 41 nt |
| GC content | 53.7% | Molecular weight | 12,668.16 Da |
| Molarity of 1 μg/μl solution | 78.94 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Target detection |
| Sequence | GAAGACGAGCGGCGAGTGTTATTACGCTTGGAAACAACCCC | ||
| Applications | Early detection, Targeted therapy, and guided surgery of glioblastoma leading to effective treatment of glioblastoma | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| ATCC ID | CRL-5800 | Cell name | Lung Adenocarcinoma (H23) |
| Category | Human cells | Organism | Homo sapiens, human |
| Product type | N/A | Cell type | N/A |
| Morphology | epithelial | Tissue | Lung |
| Disease | Adenocarcinoma; Non-small cell lung cancer | Product format | Frozen |
| Antigen expression | N/A | Genes expressed | Myc+; src+; abl+; erb+; ras+; sis- |
| Cell description | N/A |
|
|
| Application(s) | 3D cell culture; Cancer research; High-throughput screening; Toxicology | ||
Activity data
|
No relevant experimental diagram |
Interaction ID | 862 |
| Target type | Cell | |
| Target unique ID | CRL-5800 | |
| Activity | 60 nM | |
| Binding Conditions/Buffer | 0.45% w/v glucose 5 mM MgCl2 in PBS 1 mg/mL tRNA 1 mg/mL BSA incubate with cells for 30 minutes |
|
| Assay | N/A |
|
| PubMed ID | 23082117 | |
|
No relevant experimental diagram |
Interaction ID | 864 |
| Target type | Cell | |
| Target unique ID | CRL-5800 | |
| Activity | 55 nM | |
| Binding Conditions/Buffer | 0.45% w/v glucose 5 mM MgCl2 in PBS 1 mg/mL tRNA 1 mg/mL BSA incubate with cells for 30 minutes |
|
| Assay | N/A |
|
| PubMed ID | 23082117 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_778 | DNA | GAAGACGAGCGGCGAGTGTTATTACGCTTGGAAACAACCCC | |
| Apta_964 | DNA | CACGAGAGGGGTGAGGTGTCGTATTAGAGGGAAGCTCCAA | |
| Apta_641 | DNA | TTCAAGACGATGCCTGGCGCGAGTTACACACTTGCATGGAGCTGG | |
| Apta_179 | DNA | TCGGTTGAGTGAGATTAGTCAAATGTTTAGAAGACAACCTC | |
| Apta_943 | DNA | GAAGGTGGAGCGGTTAGGATACTAGCGAATGAAGACACGTTAGCTGATCT | |
| Apta_359 | DNA | GGGAACGCGTTATTGGGTAACTTCCC | |
| Apta_1146 | DNA | GGGACAGGGAGTGCGCTGCTCCCC | |
| Apta_635 | DNA | TAGCCGGATCGCGCTGGCCAGATGATATAAAGGGTCAACCCCCCA | |
| Apta_638 | DNA | TAGCCGGATCGCGCTGGCCAGATGATATAAAGGGTCAACCCCCCG | |
| Apta_585 | DNA | GATCGAGGGCAGCGATAGCTGGGCTAATAAGGTTAGCCCCATCGGTC | |
| Apta_987 | DNA | GTACTGTCAATTGGAAGTGGTGTTACGTTGTGTAGTCAAATCAGTGC | |
| Apta_668 | RNA | GGAACCCAACUAGGCGUUUGAGGGGAUUCGGCCACGGUAACAACCCCUC | |
| Apta_607 | DNA | TGAGGCGGGTGGGTGGGTTGAATATGCTGATTACCCCATCGGAGAACGTTAAGGCGCTTC | |
| Apta_71 | DNA | GGCGAGTAGGTTGGTGTGGTTGGGGCTCGCC | |
| Apta_73 | DNA | GGGGAGTAGGTTGGTGTGGTTGGGGCTCCCC | |
| Apta_385 | DNA | ACAGATACGGAACTACAGAGGTCAATTACGGTGGCCACGC | |
| Apta_928 | DNA | GATTGAGTAGATAGTGGTTCTGTACGTAGTGAAAGAGTGG | |
| Apta_1216 | DNA | AGCGTCCTCTGCATAGTGATTTACGCTATGTGTACAGTGC | |
| Apta_1270 | DNA | GTATATCAAGCATCGCGTGTTTACACATGCGAGAGGTGAA | |
| Apta_636 | DNA | TAGCCGGATCGCGCTGGCCAGATGATATAAAGGGTCAGCCCCCCA |
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