AptaDB: Result

Interaction ID: 249

Aptamer sequence: GCUUCCGGAGAGAAGGGUCAAAGUUGUGCGGGAGUGUGUUGUGG

Target unique ID: P07975

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_244	HA12-14	Protein	HEMA_INCJH	N/A	20 mM sodium phosphate, pH 7.5, 500 mM NaCl and 20 mM imidazole	24835440

Aptamer Sequence:	GCUUCCGGAGAGAAGGGUCAAAGUUGUGCGGGAGUGUGUUGUGG
The optimal secondary structure in dot-bracket notation:	(((((((.....................))))))).........
The centroid secondary structure in dot-bracket notation:	(((((((.....................))))))).........

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_244	Description	HA12-14
Aptamer chemistry	RNA	Length	44 nt
GC content	56.8%	Molecular weight	14,609.47 Da
Molarity of 1 μg/μl solution	68.45 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted therapy
Sequence	GCUUCCGGAGAGAAGGGUCAAAGUUGUGCGGGAGUGUGUUGUGG
Applications	Suppressed viral infection

Type	Detail	Type	Detail
Uniprot ID	P07975	Protein name	Hemagglutinin-esterase-fusion glycoprotein (HEF) (EC 3.1.1.53) [Cleaved into: Hemagglutinin-esterase-fusion glycoprotein chain 1 (HEF1); Hemagglutinin-esterase-fusion glycoprotein chain 2 (HEF2)]
Gene name(s)	HE	Organism	Hemagglutinin-esterase-fusion glycoprotein (HEF) (EC 3.1.1.53) [Cleaved into: Hemagglutinin-esterase-fusion glycoprotein chain 1 (HEF1); Hemagglutinin-esterase-fusion glycoprotein chain 2 (HEF2)]
Status	reviewed in uniprot database	Length	655
Mass	72,131	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	Reaction=H2O + N-acetyl-9-O-acetylneuraminate = acetate + H(+) + N-acetylneuraminate
PDB ID(s)	1FLC;6WKO;
Function	Binds to the N-acetyl-9-O-acetylneuraminic acid residues on the cell surface, bringing about the attachment of the virus particle to the cell.

	Interaction ID	249
	Target type	Protein
	Target unique ID	P07975
	Activity	N/A
	Binding Conditions/Buffer	20 mM sodium phosphate, pH 7.5, 500 mM NaCl and 20 mM imidazole
	Assay	The antiviral effect of selected aptamers on the viability of MDCK cells infected by the influenza virus was evaluated using the MTT method. MDCK cells were infected with influenza virus H3N2 at an MOI of 0.1 TCID50 and then treated with the selected RNA aptamers (5 pmol). The highest protective activity was observed by HA12-16, as compared with other selected RNA aptamers
	PubMed ID	24835440

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_242	RNA	GCUUCCAGGAAGAGAAGUGUCAUGUGAUCAGUCCUGGUGGGUGG	65.91%
Apta_241	RNA	GCUUGACGGAGAUCAAGGGCGAGUCUCAUACCAAGUUGAUGGGG	61.36%
Apta_243	RNA	GCUUGAAGCUAGCAGUAAGACUAGCGCUACGGAUGGGUGUGCG	54.55%
Apta_925	RNA	GCUCGGCCAGGUCGCGGGUGGAUGUGAGGG	54.55%
Apta_230	RNA	GGGAGGACGAUGCGGGCGUGAAUGGUGUUGUGAGG	52.27%
Apta_703	RNA	GGGCGGAUGAGACGCUUGGCGUGUGCUGUGGAGAGUCAUCCG	52.27%
Apta_926	RNA	UCGGCAGCGGUUAGAGAGGUGGUGUGCGGG	52.27%
Apta_1189	DNA	GCGGGCGGGGGGAGGGCGGCCGTGGGCTGCGAGTGGGAGG	52.27%
Apta_1168	RNA	GCGACUACGGUGAGGGUCGGGUCCAGUAGCUUCGGCUACUGUUGAGUAGAGUGUGGGCUCCGUAGUCGC	52.17%
Apta_431	RNA	CAGGCAUGCCUAGCUAAGCAGCCCAUGGCUUAUGCGCGGAAUAUUGGCUUCCGUUC	51.79%
Apta_150	DNA	CCGTAGGTTCGGGGCGGAGTGGTCCGGAAGGTGGCGTGG	50.00%
Apta_218	RNA	AGCCGCGAGGGGAGGGAUAGGGUAGGGCGCGGCU	50.00%
Apta_575	RNA	AGAGCUUGACGGUCCCGAGAGUCGAGCCCAAGCUGACACUGGACCUUUGCGGACCACGUGUUGAUCGUCG	50.00%
Apta_594	RNA	GGGCUAUUGCUGGAGGGGCGCCACAUGAAAGUGGUGGUUGGGUGCGGUCGGCGAUAGCUC	50.00%
Apta_633	RNA	GGCAUCGGAAAGUGGGUUGAUGUAAGUAACAGGCGAUGCC	50.00%
Apta_830	DNA	GACGGTGGCAGGGAAAGGGGTCGGGCATATGGCGGAGGGG	50.00%
Apta_927	RNA	UUACAAUGAGGUGGGACGCGGGUUGUGGUG	50.00%
Apta_1167	RNA	GGCGCGAGGAAGGAGGUCUGAGGAGGUCACUGCGCC	50.00%
Apta_520	RNA	GGGAGAUCUACGGAUCUCAGGGCUCUUACGGGAGCUACAUGGAAGGAGUCCAUGUGU	49.12%
Apta_312	RNA	GGGAAAAGAGACGGCCGGCGCCAUAGCCGAGAUCCGAGGUGUUGCCGAGAA	49.02%

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