Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 927
Aptamer sequence: TGAGCCCAAGCCCTGGTATGGTGTGCCTGTCGTTGTATTGGTCGGTAGGGATCGGAGTGGGGCAGGTCTACTTTGGGATC
Target unique ID: BAA-2587-PACK
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_841 Apt-6 Cell Enterohemorrhagic Escherichia coli O157:H7 (E. coli O157:H7) 36.67 nM 1× BB, 50 mmol/L Tris-HCl (pH 7.4), 5 mmol/L KCl, 100 mmol/L NaCl, and 1 mmol/L MgCl2 29756774
Structure information of aptamer
Aptamer Sequence: TGAGCCCAAGCCCTGGTATGGTGTGCCTGTCGTTGTATTGGTCGGTAGGGATCGGAGTGGGGCAGGTCTACTTTGGGATC
The optimal secondary structure in dot-bracket notation: ....(((((..........((((.(((((((......((((((......)))))).....))))))).)))))))))...
The centroid secondary structure in dot-bracket notation: ....(((((..........((((.(((((((......((((((......)))))).....))))))).)))))))))...
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_841 Description Apt-6
Aptamer chemistry DNA Length 80 nt
GC content 57.5% Molecular weight 24,922.87 Da
Molarity of 1 μg/μl solution 40.12 μM Number of G-quadruplexes 2
G-Score 21 Function Targeted binding
Sequence TGAGCCCAAGCCCTGGTATGGTGTGCCTGTCGTTGTATTGGTCGGTAGGGATCGGAGTGGGGCAGGTCTACTTTGGGATC
Applications Binding to Enterohemorrhagic Escherichia coli O157:H7 (E. coli O157:H7)
Target information
Type Detail Type Detail
ATCC ID BAA-2587-PACK Cell name Enterohemorrhagic Escherichia coli O157:H7 (E. coli O157:H7)
Category Bacteria Organism N/A
Product type Reporter-labeled bacterium Cell type N/A
Morphology N/A Tissue N/A
Disease N/A Product format Frozen
Antigen expression N/A Genes expressed N/A
Cell description A kit containing reporter-labeled Escherichia coli designed to distinguish control strain cross-contamination from true contamination in test samples. This product is appropriate for use as a positive control in quality control assays for E. coli.
Application(s) Assay development; Food testing; High-throughput screening
Activity data

No relevant experimental diagram

 Interaction ID 927
Target type Cell
Target unique ID BAA-2587-PACK
Activity 36.67 nM
Binding Conditions/Buffer

1× BB, 50 mmol/L Tris-HCl (pH 7.4), 5 mmol/L KCl, 100 mmol/L NaCl, and 1 mmol/L MgCl2
Assay

N/A
PubMed ID 29756774
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_842 DNA TGAGCCCAAGCCCTGGTATGTGTGGGGTCCTGGATTATGTTTAGCGTCTTTCGCAGTGGGGGCAGGTCTACTTTGGGATC 76.25%
Apta_836 DNA TGAGCCCAAGCCCTGGTATGTTACAGTATGCTACCTCTACTTGAAGGTTGGTCGACGCGGGGCAGGTCTACTTTGGGATC 73.75%
Apta_837 DNA TGAGCCCAAGCCCTGGTATGTGATCGGTGACGAGGGTGCGGGGCGGGGGGTGAGGCACAGGGCAGGTCTACTTTGGGATC 72.50%
Apta_838 DNA TGAGCCCAAGCCCTGGTATGTAGTAATGGTGCGTACAGGCGACGGGGTCCAGGCTGGAGGGGCAGGTCTACTTTGGGATC 72.50%
Apta_840 DNA TGAGCCCAAGCCCTGGTATGCGGATAACGAGGTATTCACGACTGGTCGTCAGGTATGGTTGGCAGGTCTACTTTGGGATC 71.25%
Apta_843 DNA TGAGCCCAAGCCCTGGTATGTCACATCCGGGTTCTTTGCGAACGCGTTTCCGCAGTCTCAGGCAGGTCTACTTTGGGATC 68.75%
Apta_844 DNA TGAGCCCAAGCCCTGGTATGTTGCGGGAGTCTAGCGGGCCACACTTTTATAGGTTCGCAGGGCAGGTCTACTTTGGGATC 68.75%
Apta_839 DNA TGAGCCCAAGCCCTGGTATGAGCCCACGGAACACTGGTCGCGCCCACTGGTTTCTATATTGGCAGGTCTACTTTGGGATC 67.50%
Apta_568 DNA CCCAGCAGCACTGGTAGTGAGGCAGTTCACCGGTGGGGCGGTGAGTTTGGCTGCTATTTAT 55.00%
Apta_465 DNA AGCTGACACAGCAGGTTGGTGGGGGTGGCTTCCAGTTGGGTTGACAATACGTAGGGACACGAAGTCCAACCACGAGTCGAGCAATCTCGAAAT 52.69%
Apta_31 DNA ATACCAGTCTATTCAATTGGGCCCGTCCGTATGGTGGGTGTGCTGGCCAGATAGTATGTGCAATCA 52.50%
Apta_67 DNA AGATGCCTGTCGAGCATGCTGTTGTGGTAGGGTTAGGGATGGTAGCGGTTGTAGCTAAACTGCTTTGTCGACGGG 52.50%
Apta_408 DNA GCAATGGTACGGTACTTCCGTCATCAGCTTGTGATGTGGATGCGAACTGCAAAAGTGCACGCTACTTTGCTAA 52.50%
Apta_508 DNA GCGGGCGATCGGCGGGGGGTGCGTGCGCTCTGTGCCAGGGGGTGGGACAGATCATATGGGGGTGCT 52.50%
Apta_650 DNA GCACACCGATGGCGGTCCTGTTTAGGGGGTGTGCGTGGGGAGGTGCGGTTGC 52.50%
Apta_942 DNA AGTGGGTAAGGTCTGGTGGATTGTGGACGGGGGGCGGGGCAGTGGCTTGA 52.50%
Apta_1071 DNA TTGAAGTGACTCCGCACTGGGTGGGTGGGAGGGTCGTGCGGCTGGTCATAGCAGGGT 52.50%
Apta_953 DNA TCACCTCATACGACTCACTATAGCGGATCCGAGCCGGGGTGTTCTGTTGGCGGGGGCGGTGGGCGGGCTGGCTCGAACAAGCTTGC 52.33%
Apta_812 DNA ATAGGAGTCACGACGACCAGAATAGAGATATGACAGCGGGGAAGGTTAAGAGGCGCTAGGAGTATGTGCGTCTACCTCTTGACTAAT 51.72%
Apta_454 DNA AGCTCCAGAAGATAAATTACAGGTGAGGGCGGGCGGGTGGTTGTAATATGATCGAATGGTATATGTGTGTTTGCAACTAGGATACTATGACCCCG 51.58%

ABOUT

AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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