AptaDB: Result

Interaction ID: 312

Aptamer sequence: CCUCCGGCUCAUACCUUUUCGAAGACAAGCUUCGACAGGAGG

Target unique ID: P11672

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_303	Oligo569	Protein	NGAL_MOUSE	340 nM	50 mM KH2PO4, 150 mM NaCl, 5 mM MgCl2, pH 7.4	22946879

Aptamer Sequence:	CCUCCGGCUCAUACCUUUUCGAAGACAAGCUUCGACAGGAGG
The optimal secondary structure in dot-bracket notation:	(((((((......))...((((((.....))))))..)))))
The centroid secondary structure in dot-bracket notation:	(((((((......))...((((((.....))))))..)))))

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_303	Description	Oligo569
Aptamer chemistry	RNA	Length	42 nt
GC content	54.8%	Molecular weight	13,644.94 Da
Molarity of 1 μg/μl solution	73.29 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Aptasensor
Sequence	CCUCCGGCUCAUACCUUUUCGAAGACAAGCUUCGACAGGAGG
Applications	Detection of Lipocalin-2

Type	Detail	Type	Detail
Uniprot ID	P11672	Protein name	Neutrophil gelatinase-associated lipocalin (NGAL) (Lipocalin-2) (Oncogene 24p3) (24p3) (SV-40-induced 24p3 protein) (Siderocalin LCN2) (p25)
Gene name(s)	Lcn2	Organism	Neutrophil gelatinase-associated lipocalin (NGAL) (Lipocalin-2) (Oncogene 24p3) (24p3) (SV-40-induced 24p3 protein) (Siderocalin LCN2) (p25)
Status	reviewed in uniprot database	Length	200
Mass	22,875	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	3S26;3U9P;
Function	Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development.

	Interaction ID	312
	Target type	Protein
	Target unique ID	P11672
	Activity	340 nM
	Binding Conditions/Buffer	50 mM KH2PO4, 150 mM NaCl, 5 mM MgCl2, pH 7.4
	Assay	The mLcn2 aptamer (oligo569) was titrated into 5 μM 6xHis-mLcn2 by ITC
	PubMed ID	22946879

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_51	RNA	GCCUUAGUAACGUGCUUUGAUGUCGAUUCGACAGGAGGC	59.52%
Apta_269	RNA	AACGCUCAACGUCAGAAGGCAACGUAUAGGCAAGCACACUUCGACAUGAGGC	55.77%
Apta_425	RNA	UGCCGCCAUAUCACACGGAUUUAAUCGCCGUAGAAAAGCAUGUCAAAGCCG	52.94%
Apta_93	RNA	CUCCGCUAUCUAACGUAUGAUAGAAUGGAUGACUA	52.38%
Apta_153	RNA	CCACAGUUCAGAGGCACCGCGAACAAAACGCAAGACAGAGUGCCGACAAGAAC	50.94%
Apta_588	RNA	CCGGUGCGCAUAACCACCUCAGUGCGAGCAAGGAA	50.00%
Apta_603	RNA	CUUGUCUGAACAAUUUCGUCUGCCUUUGCGUUCCGACGGC	50.00%
Apta_634	RNA	GGUCUUACGUCGUUCGCGACUAUUGGGAGACC	50.00%
Apta_658	RNA	CUGCGAUCAGGGGUAAAUUUCCGCGCAGGCUCCACGCCGC	50.00%
Apta_707	RNA	GGCCUAAAACAUACCAGAUUUCGAUCUGGAGAGGUGAAGAAUUCGACCACCUAGGCCGGU	50.00%
Apta_856	RNA	UGGGCCGGAGGUUAGCUUGCCCAUGGCAAGCAGGGCGCCACGGACCCA	50.00%
Apta_873	DNA	CCACCGAGCCTACCACATGTGACATCCCAGGACATAGCTG	50.00%
Apta_271	RNA	CUGUCGAGCAUGCUCAGGGUAACGUAUAGUCAAGACACCUCAAGUGUAACU	49.02%
Apta_426	RNA	UGCCGCUAUAAUGCACGGAUUUAAUCGCCGUAGAAAAGCAUGUCAAAGCCG	49.02%
Apta_292	RNA	CUACGAACUCAUGACACAAGGAUGCAAUCUCAUCCCGCCAGCCCACCGU	48.98%
Apta_3	DNA	CCTGCCTTTGGCAGCCGGGCGAAGGCAACCCGACACCGACAGC	48.84%
Apta_1249	RNA	AAUCGGCCCAAUUUUACCUUCGGACUUCAGUAUCUAGUGUGGCUUUAAAUCAUAGC	48.21%
Apta_155	RNA	GACCCAGGGCCGGGACGCAAAGCAGCCAAAACAGACGGCCCCAGUCAGGGAGUU	48.15%
Apta_1141	RNA	ACGAGUCUGGUCCUUCCUUUGACGACUAUUGUUUGGUAUCGAUCAACAACAA	48.08%
Apta_136	RNA	CGUGUCGUCCUGGUACGAUUUGGUAUAUAACCGUGGCUUUUCGACA	47.83%

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