AptaDB: Result

Interaction ID: 830

Aptamer sequence: AGCTTGAGGGTGGGCGGGTGGACGCGGTAGTGGTATATAGGTCGG

Target unique ID: CRM-CCL-185

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_748	S11.c	Cell	vaccinia virus–infected and–uninfected lung cancer A549 cells	65.7 nM	5 mmol MgCl2 and 4.5 g glucose into 1 L of Dulbecco's PBS without calcium and magnesium	19684903

Aptamer Sequence:	AGCTTGAGGGTGGGCGGGTGGACGCGGTAGTGGTATATAGGTCGG
The optimal secondary structure in dot-bracket notation:	............(((..(((..(((....)))...)))..)))..
The centroid secondary structure in dot-bracket notation:	.......................((....))..............

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_748	Description	S11.c
Aptamer chemistry	DNA	Length	45 nt
GC content	62.2%	Molecular weight	14,190.06 Da
Molarity of 1 μg/μl solution	70.47 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Target recognition
Sequence	AGCTTGAGGGTGGGCGGGTGGACGCGGTAGTGGTATATAGGTCGG
Applications	Recognition of subtype non-small cell lung cancer

Type	Detail	Type	Detail
ATCC ID	CRM-CCL-185	Cell name	vaccinia virus-infected and-uninfected lung cancer A549 cells
Category	Human cells	Organism	Homo sapiens, human
Product type	Certified reference material	Cell type	epithelial cell
Morphology	epithelial	Tissue	Lung
Disease	Carcinoma	Product format	Frozen
Antigen expression	N/A	Genes expressed	N/A
Cell description	N/A
Application(s)	Assay development

No relevant experimental diagram	Interaction ID	830
	Target type	Cell
	Target unique ID	CRM-CCL-185
	Activity	65.7 nM
	Binding Conditions/Buffer	5 mmol MgCl2 and 4.5 g glucose into 1 L of Dulbecco's PBS without calcium and magnesium
	Assay	N/A
	PubMed ID	19684903

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_693	DNA	CGAGTTGAGCCGGGCGCGGTACGGGTACTGGTATGTGTGG	66.67%
Apta_1230	DNA	GGCGATAGGCAGTGTTGCGGGGTCGGAGAGCGAGGTAATAGCGTGTATGGGTGCTGTGTG	60.00%
Apta_940	DNA	TGTGCGGGGGAGGGGAGTGGCGCTGTTGGTGCGGGTATAGCGCGTGGTGT	58.00%
Apta_149	DNA	CCGTAGGTTCGGGGTCGGAGTGGTCCGGAAGATGGCGTGG	57.78%
Apta_264	DNA	GGCGTTGTCGGGCGCAGGTGTAGGCCTCGTGGTGGTGGGT	57.78%
Apta_746	DNA	AGAGTGGGGGGGTGGGTGGATTTGACAGGTGGCATGCTGGAGAGT	57.78%
Apta_869	DNA	ACTAGGACGCTTGGGAGGGGGGGTGGGGTGTCCGGTCGCG	57.78%
Apta_1138	DNA	GCAGTGTGGGCGAATCTATGCGTACCGTTCGATATCGTG	57.78%
Apta_1189	DNA	GCGGGCGGGGGGAGGGCGGCCGTGGGCTGCGAGTGGGAGG	57.78%
Apta_1254	DNA	GGCAGTGTGGGCGAATCTATGCGTACCGTTCGATATCGTG	57.78%
Apta_464	DNA	GTCAGATTCCACTATAGTAGGTTGGGTAGGGTGGTCGCAGTGGATGATATGTCGTAGGGG	56.67%
Apta_988	DNA	AAGGATGACTGACGACGATGGGAATGGAGAGGAAGGGGATATAGGTATGTTGGATGTAG	55.93%
Apta_124	DNA	GGTGGTGGGGGGGGTTGGTAGGGTGTCTTC	55.56%
Apta_144	DNA	GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCGATAGAGGTAG	55.56%
Apta_148	DNA	CCGTAGGTTCGGGGTCGGAGTGGTCCGGAAGGTGGCGTGG	55.56%
Apta_150	DNA	CCGTAGGTTCGGGGCGGAGTGGTCCGGAAGGTGGCGTGG	55.56%
Apta_482	DNA	AGCAAGGTTTGGTGTTGGTTGGTTGCTGGTTTTGGTTTC	55.56%
Apta_488	DNA	CGACCAGGGTGGGAGGGGGACGGGCTGGCGGCGCGCGGTAATG	55.56%
Apta_565	DNA	GCGGGGTTGGGCGGGTGGGTTCGCTGGGCAGGGGGCGAGTG	55.56%
Apta_590	DNA	ACCATCTGTGTAAGGGGTAAGGGGTGGGGGTGGGTACGTCT	55.56%

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