Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 7
Aptamer sequence: CCAGTCTCCCGTTTACCGCGCCTACACATGTCTGAATGCC
Target unique ID: I0IT65
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_7 | Gli4 | Protein | I0IT65_WHEAT | 61 nM | 50 mM TRIS-HCl, 0.25 M NaCl, 5mM MgCl2 | 24502317 |
Structure information of aptamer
| Aptamer Sequence: | CCAGTCTCCCGTTTACCGCGCCTACACATGTCTGAATGCC |
| The optimal secondary structure in dot-bracket notation: | .........((((((..(((........))).)))))).. |
| The centroid secondary structure in dot-bracket notation: | .........(((((....(..........)...))))).. |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_7 | Description | Gli4 |
| Aptamer chemistry | DNA | Length | 40 nt |
| GC content | 57.5% | Molecular weight | 12,103.77 Da |
| Molarity of 1 μg/μl solution | 82.62 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted binding |
| Sequence | CCAGTCTCCCGTTTACCGCGCCTACACATGTCTGAATGCC | ||
| Applications | Detect hordeins and secalins as well as avenins withlower affinity and discriminates them from rice, soya, and maize proteins | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | I0IT65 | Protein name | Alpha-gliadin (Alpha/beta-gliadin) (CSD_alpha gliadin 11) (Gliadin alpha-D12) |
| Gene name(s) | Gli-2 gli-2 Gli-D2 Gli-D2-1 | Organism | Alpha-gliadin (Alpha/beta-gliadin) (CSD_alpha gliadin 11) (Gliadin alpha-D12) |
| Status | unreviewed in uniprot database | Length | 282 |
| Mass | 32,206 | Activity regulation | N/A |
| Involvement in disease | N/A | Catalytic activity | N/A |
| PDB ID(s) | N/A |
 |
Function | N/A |
Activity data
 |
Interaction ID | 7 |
| Target type | Protein | |
| Target unique ID | I0IT65 | |
| Activity | 61 nM | |
| Binding Conditions/Buffer | 50 mM TRIS-HCl, 0.25 M NaCl, 5mM MgCl2 |
|
| Assay | Competitive assay curve. Analytical signals expressed as percentage of the maximum intensity current as a function of the PWG concentration in the presence of [gli4] = 250 nM |
|
| PubMed ID | 24502317 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_1263 | DNA | CCCCGCTGCCCGTGTTCCGTCCTCCCTTGCTGTGTGTGCG | |
| Apta_142 | DNA | CGCACATCAGTTTAGCAGCGCCAAAGGCGGAAGCGCA | |
| Apta_189 | DNA | AGGAACCCTTTACCTCTGAGATCTCGGAAT | |
| Apta_190 | DNA | AGGAACCCTTTAGGTCTGAGATCTCGGAAT | |
| Apta_687 | DNA | GGGACAGCGGAGGTCTCCCACCCACCGGCCCCTGCGGCACACCAACCTGTATGGATGCG | |
| Apta_186 | DNA | AGGAACGCTTTAGGTCTGAGATCTCGGAAT | |
| Apta_187 | DNA | ACCAACCCTTTACCTCTGAGATCTCCCAAT | |
| Apta_227 | DNA | ACGCGCCTATGGTGCATCGCATGCCGACC | |
| Apta_249 | DNA | CCCTGTTCCCCCTCCGCCGCTTGACTCTCC | |
| Apta_253 | DNA | CCCTGTTCGCTATCCCCCATCCCCCGATTGC | |
| Apta_307 | DNA | CATTCACCATGGCCCCTTCCTACGTATGTTCTGCGGGTG | |
| Apta_437 | DNA | TCACAGTCCCGGTGCCGCACTAAAACCCATTGTTGTGCGA | |
| Apta_1027 | DNA | GGAGCTACGTTTAGCGAGTCCGACGCTCGGTGCCTCTTC | |
| Apta_238 | DNA | CACACATTCCCGTCCTCGATACGTCTAGGCTTAGTGCCACTTGCTTAATC | |
| Apta_514 | DNA | ACTCAGCACGCGTGTAGGGAAAACCAACCGCCCACAAGCTGCATGCAACG | |
| Apta_356 | DNA | CGGGGGGTACGTGCCCGTGTTGCTCGGCGGCCCCCTCGCTTATTTTTGTGTGCCCTTT | |
| Apta_969 | DNA | TAACCACCCCACCTCGCTCCCGTGACACTAATGCTAATTCCAA | |
| Apta_833 | DNA | ATTTCGCCCCCGTGTTCCGACTGGTATCTTCACGTCTTCGAGTGT | |
| Apta_845 | DNA | CCAAACGCGCTGGTTTGGAATGCTTAGAGTTTGATCGATGCCTAA | |
| Apta_1033 | DNA | CACAGGTTCCAGGTAATACCTAAGGGTATGCTCTCGCCTATTATATGGAGCAC |
DEV TOOL
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