Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 916
Aptamer sequence: GACGGTGGCAGGGAAAGGGGTCGGGCATATGGCGGAGGGG
Target unique ID: 25922
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_830 | P12-17 | Cell | Escherichia Coli (E.Coli) | 56.33 nM | PBS containing 1.4 mM MgCl2 | 27104834 |
Structure information of aptamer
| Aptamer Sequence: | GACGGTGGCAGGGAAAGGGGTCGGGCATATGGCGGAGGGG |
| The optimal secondary structure in dot-bracket notation: | ...................((((......))))....... |
| The centroid secondary structure in dot-bracket notation: | ........................................ |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_830 | Description | P12-17 |
| Aptamer chemistry | DNA | Length | 40 nt |
| GC content | 70.0% | Molecular weight | 12,678.11 Da |
| Molarity of 1 μg/μl solution | 78.88 μM | Number of G-quadruplexes | 3 |
| G-Score | 58 | Function | Aptasensor |
| Sequence | GACGGTGGCAGGGAAAGGGGTCGGGCATATGGCGGAGGGG | ||
| Applications | Diagnosis of MNEC-borne pathologies | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| ATCC ID | 25922 | Cell name | Escherichia Coli (E.Coli) |
| Category | Bacteria | Organism | N/A |
| Product type | Preceptrol culture | Cell type | N/A |
| Morphology | N/A | Tissue | N/A |
| Disease | N/A | Product format | Freeze-dried |
| Antigen expression | N/A | Genes expressed | N/A |
| Cell description | This strain does not produce verotoxin. This organism is a CLSI control strain for antimicrobial susceptibility testing. Used in susceptibility disc testing of neomycin, colistin [colimycin], kanamycin, cephalexin, gentamicins, cefamandole, cephalothin, tetracycline, cephaloglycin, cephaloridine [cephalomycin], nalidixic acid, and chloramphenicol. |
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|
| Application(s) | Bioinformatics; Food testing; Media testing; Quality control | ||
Activity data
 |
Interaction ID | 916 |
| Target type | Cell | |
| Target unique ID | 25922 | |
| Activity | 56.33 nM | |
| Binding Conditions/Buffer | PBS containing 1.4 mM MgCl2 |
|
| Assay | Binding affinity of selected pool 12 aptamers to E coli ATCC 25922 |
|
| PubMed ID | 27104834 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_579 | DNA | ACTGAGGGCACGGACAGGAGGGGGAGAGATGGCGTGAGGT | |
| Apta_264 | DNA | GGCGTTGTCGGGCGCAGGTGTAGGCCTCGTGGTGGTGGGT | |
| Apta_940 | DNA | TGTGCGGGGGAGGGGAGTGGCGCTGTTGGTGCGGGTATAGCGCGTGGTGT | |
| Apta_679 | DNA | CAGGTGGCAGATCGGGTGTGGGTGGCCTGG | |
| Apta_1195 | DNA | GGGGTTGGGGGGAGGCGGGGAATGGGGG | |
| Apta_754 | DNA | GGGCGGGGAGTAGGGAGAGGGGTTTCCATCGGCGACAGAGGAGTTATGTGTGT | |
| Apta_565 | DNA | GCGGGGTTGGGCGGGTGGGTTCGCTGGGCAGGGGGCGAGTG | |
| Apta_1062 | DNA | GGGGTCTTGCAGGTCCCGTAGGAGGGGCCATTGGAGTGGGG | |
| Apta_488 | DNA | CGACCAGGGTGGGAGGGGGACGGGCTGGCGGCGCGCGGTAATG | |
| Apta_10 | DNA | GGGGGGGGTGGCTGGGCGGGGTGGG | |
| Apta_14 | DNA | AGCGGGGGGGGCTGGGCTGGGTGGG | |
| Apta_76 | DNA | CAGCGCTAGGGCTTTTAGCGTAATGGGTAGGGTGGTGCGGTGCAGATATCGGAATTGGTG | |
| Apta_150 | DNA | CCGTAGGTTCGGGGCGGAGTGGTCCGGAAGGTGGCGTGG | |
| Apta_674 | DNA | GCACGATGGGGAAAGGGTCCCCCTGGGTTG | |
| Apta_693 | DNA | CGAGTTGAGCCGGGCGCGGTACGGGTACTGGTATGTGTGG | |
| Apta_831 | DNA | CCGCCCAGCGGGGGTAGGGCCGGACGTAGGAGGAGCTGCG | |
| Apta_1189 | DNA | GCGGGCGGGGGGAGGGCGGCCGTGGGCTGCGAGTGGGAGG | |
| Apta_1230 | DNA | GGCGATAGGCAGTGTTGCGGGGTCGGAGAGCGAGGTAATAGCGTGTATGGGTGCTGTGTG | |
| Apta_991 | DNA | AGCAGTGAAGAGGCGAGAGATTTATGAGGGTAGCGTCCACATATGCCAGGATCGTGTTG | |
| Apta_1046 | DNA | TCGCATGGAGGGCAGAGCCGCCTGCCGGGATCCGGCCCTCTTGGGCGGGC |
DEV TOOL
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