Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 962
Aptamer sequence: GTTGCGAAAGACAACGAATGCTTTGCCTGCCATAATTTGC
Target unique ID: Other-12
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_876 | WKB-14 | Other | P48 protein of Mycoplasma bovis (M. bovis) | 15.61 nM | P48 protein dissolved in 100 μL of 0.05 carbonate-bicarbonate buffer | 24417693 |
Structure information of aptamer
| Aptamer Sequence: | GTTGCGAAAGACAACGAATGCTTTGCCTGCCATAATTTGC |
| The optimal secondary structure in dot-bracket notation: | ((.((.((((.((.....))))))))..)).......... |
| The centroid secondary structure in dot-bracket notation: | ...(((..((.((.....)))).))).............. |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_876 | Description | WKB-14 |
| Aptamer chemistry | DNA | Length | 40 nt |
| GC content | 45.0% | Molecular weight | 12,294.93 Da |
| Molarity of 1 μg/μl solution | 81.33 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Target detection |
| Sequence | GTTGCGAAAGACAACGAATGCTTTGCCTGCCATAATTTGC | ||
| Applications | Detection of antibodies to mycoplasma bovis in serum | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Target ID | Other-12 | Name | P48 protein of Mycoplasma bovis (M. bovis) |
Activity data
 |
Interaction ID | 962 |
| Target type | Other | |
| Target unique ID | Other-12 | |
| Activity | 15.61 nM | |
| Binding Conditions/Buffer | P48 protein dissolved in 100 μL of 0.05 carbonate-bicarbonate buffer |
|
| Assay | Dose-dependent i-ELAA to determine the dissociation constant (Kd) of aptamers |
|
| PubMed ID | 24417693 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_776 | DNA | AGTGGTCGAACTACACATCCTTGAACTGCGGAATTATCTAC | |
| Apta_1162 | DNA | AGAGGCTACAGCGATAAGTCGACATTGCTGACCGTACCTAGTAATACGT | |
| Apta_1213 | DNA | GGACTTGAAGAAAAGGGCGACACGCGAAGTCGATGTCGCGTCTGCCTGAAGTCA | |
| Apta_883 | DNA | CGTACCGGCCAGTGATTACGACGAGACGAGCTTATGCGTATTGATGCCTAACTATCTACA | |
| Apta_1171 | DNA | TTTTTTTTTTTCGCAGCACCCAAGAACAAGGACTGCTTAGGATTGCGATAGGTTCGG | |
| Apta_631 | DNA | GGGATCGAAACGTAGCGCCTTCGATCCC | |
| Apta_781 | DNA | GTACGCCGCAAGACGAGTTGTGTATAAGCCGGC | |
| Apta_877 | DNA | GTGCGAAGCGCCTCCACTGTACATCCACTCCTTCTGCC | |
| Apta_1206 | DNA | GGCAGCAGCAATGTAACACTGTGTGTATGTGTTGG | |
| Apta_1239 | DNA | GGTGGATGGGGATGCTTTGTTAGTTTGTGC | |
| Apta_1242 | DNA | GGAGCGATTAGTAGTGGTCTTGTCTTTTGC | |
| Apta_846 | DNA | GTGCAGACAGCTAGTAGTGGTGACTTTCTGTCCAAATCAAGATAA | |
| Apta_592 | DNA | CTCTCGGGACGACCACTACGCATAGTTTCTATCGCCAGGAAGGGTCGTCCC | |
| Apta_1033 | DNA | CACAGGTTCCAGGTAATACCTAAGGGTATGCTCTCGCCTATTATATGGAGCAC | |
| Apta_910 | DNA | TCACGTGCATGATAGACGGCGAAGCCGTCGAGTTGCTGTGTGCCGATGCACGTGA | |
| Apta_221 | DNA | GTCCTAGCAGACACTCGGCCATCACGCCCATTGGTTTGCA | |
| Apta_360 | DNA | GGGAACGCATACGCCGCAGTATTTCCC | |
| Apta_362 | DNA | GGGAACGCACCGATCGCAGGTTTCCC | |
| Apta_513 | DNA | TAGGGAAAACCAACCGCCCAGAACTGCAGCATAAC | |
| Apta_573 | DNA | CGTACAGTGGGCTATATTGCTGACGTAAGAGCTGCTTTCAATGCATTGGCATAGATGTCA |
DEV TOOL
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