Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 818
Aptamer sequence: ATCGTCTGCTCCGTCCAATATGATGACACCTGCATAATTTATAGTGAGTCTTGATTCACGCTGCATTTGGTGTGAGGTCGTGC
Target unique ID: CRM-CCL-185
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_736 | TV08 | Cell | vaccinia virus–infected and–uninfected lung cancer A549 cells | 2.7 nM | Dulbecco PBS, 4.5g/L glucose, 5 mmol/L MgCl2, 0.1 g/L yeast tRNA, 100 mL/L FBS, and 1 g/L BSA | 19246617 |
Structure information of aptamer
| Aptamer Sequence: | ATCGTCTGCTCCGTCCAATATGATGACACCTGCATAATTTATAGTGAGTCTTGATTCACGCTGCATTTGGTGTGAGGTCGTGC |
| The optimal secondary structure in dot-bracket notation: | ..................((((((.(((((((((.........((((((....))))))..))))...)))))...)))))). |
| The centroid secondary structure in dot-bracket notation: | .........................(((((((((.........((((((....))))))..))))...))))).......... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_736 | Description | TV08 |
| Aptamer chemistry | DNA | Length | 83 nt |
| GC content | 45.8% | Molecular weight | 25,569.35 Da |
| Molarity of 1 μg/μl solution | 39.11 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Target recognition |
| Sequence | ATCGTCTGCTCCGTCCAATATGATGACACCTGCATAATTTATAGTGAGTCTTGATTCACGCTGCATTTGGTGTGAGGTCGTGC | ||
| Applications | Detection of A549 cells | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| ATCC ID | CRM-CCL-185 | Cell name | vaccinia virus-infected and-uninfected lung cancer A549 cells |
| Category | Human cells | Organism | Homo sapiens, human |
| Product type | Certified reference material | Cell type | epithelial cell |
| Morphology | epithelial | Tissue | Lung |
| Disease | Carcinoma | Product format | Frozen |
| Antigen expression | N/A | Genes expressed | N/A |
| Cell description | N/A |
|
|
| Application(s) | Assay development | ||
Activity data
 |
Interaction ID | 818 |
| Target type | Cell | |
| Target unique ID | CRM-CCL-185 | |
| Activity | 2.7 nM | |
| Binding Conditions/Buffer | Dulbecco PBS, 4.5g/L glucose, 5 mmol/L MgCl2, 0.1 g/L yeast tRNA, 100 mL/L FBS, and 1 g/L BSA |
|
| Assay | The Kds of aptamers were determined by flow cytometry |
|
| PubMed ID | 19246617 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_740 | DNA | ATCGTCTGCTCCGTCCAATAAACCTGCATAATTTATAAGTCTAGACTGCTGCATTTGGTGTGAGGTCGTGC | |
| Apta_741 | DNA | ATCGTCTGCTCCGTCCAATAGCCTCACCCTGCATAATTTATAGACTACACTTAGGAATCGCTGCATTTGGTGTGAGGTCGTGC | |
| Apta_743 | DNA | ATCGTCTGCTCCGTCCAATATCCGAGCAAGAACTCATATTGCATTATTTATAGCTACGCGCTGCATTTGGTGTGAGGTCGTGC | |
| Apta_737 | DNA | ATCGTCTGCTCCGTCCAATAGTGCATTGAAACTTCTGCATCCTCGTTTGGTGTGAGGTCGTGC | |
| Apta_739 | DNA | ATCGTCTGCTCCGTCCAATAGCGTGCATTGGTTTACTGCATCCGTGAAACTGGGCTTTGGTGTGAGGTCGTGC | |
| Apta_791 | DNA | ATCGTCTGCTCCGTCCAATATATCAAAGGCGAATTTTGTCAAGGTGTTAAACGATAGTCCCTACCTTTGGTGTGAGGTCGTGC | |
| Apta_738 | DNA | ATCGTCTGCTCCGTCCAATACCTGCATATACACTTTGCATGTGGTTTGGTGTGAGGTCGTGC | |
| Apta_742 | DNA | ATCGTCTGCTCCGTCCAATAGGACCGATAGGAACCACGGACTGCATGTTTCTGCATTTGACGTGGTTTGGTGTGAGGTCGTGC | |
| Apta_532 | DNA | ATCCGTCACACCTGCTCTAACTTACACATAATTCTAGGTTACATCTTGCTCATATGGTGTTGGCTCCCGTAT | |
| Apta_530 | DNA | ATCCGTCACACCTGCTCTGTGTACGCCCGTATTTACGTTCCTAGCAATTGCTATGTGGTGTTGGCTCCCGTAT | |
| Apta_657 | DNA | CCTGCACCTCGTCCAGCATGCACACCGATGGCGGTCCTGTTTTACGGTAGCTTATATGCAGGGGGTGTGCGTGGGGAGGTGCGGTTGC | |
| Apta_451 | DNA | ATACCAGCTTATTCAATTGAGTAAGAGTGCACTATGTTTTACGAGCCGTTTCCTCGGCAGATAGTAAGTGCAATCT | |
| Apta_536 | DNA | ATCCGTCACACCTGCTCTCGAATAGAGTACTCGATACTGACTTTATTTACCGTCTGGTGTTGGCTCCCGTAT | |
| Apta_899 | DNA | ATCCGTCACACCTGCTCTCGAATAGAGTATTCGATACTGACTTTATTTACCGTCTGGTGTTGGCTCCCGTAT | |
| Apta_1174 | DNA | ATGAGAGCGTCGGTGTGGTAAGTATGATATCGGTGTTTATGGTGTCTGTCTTCATACTCTTGCTGTAGGAGGGTGCGGAAGTA | |
| Apta_1231 | DNA | ACCACCGGCCCGAGATAGTCTAGACCACTATGTTGTTGTGCTTACTGCTGTGTGGTGTGG | |
| Apta_800 | DNA | AAGGAGCAGCGTGGAGGATATACACCGTCATCAGAGGGAGCATCTCTAGTCAGGACTGTGATGAATTAGGGTGTGTCGTCGTGGT | |
| Apta_140 | DNA | GGCAGGAAGACAAACACGATGGGGGGGTATGATTTGATGTGGTTGTTGCATGATCGTGGTCTGTGGTGCTGT | |
| Apta_233 | DNA | AGCAGCACAGAGGTCAGATGTGAAACATAGCATATTTACTTATGTCGCCTTGCCGGTTCCTATGCGTGCTACCGTGAA | |
| Apta_511 | DNA | ATCCGTCACACCTGCTCTCAGGGGATCACATTCTTGACGGTGTGATACAGTGCCTGGTGTTGGCTCCCGTAT |
DEV TOOL
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