Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 876
Aptamer sequence: ATACCAGCTTATTCAATTCCCAAATTGCCACCACTTACAGCATGATAACATACTACATCTTTTCATCAAGATAGTAAGTGCAATCT
Target unique ID: PCS-100-011
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_792 | Aptamer N55 | Cell | Inflamed human aortic endothelial cell | 82 nM | 5 mmol/L MgCl2, 4.5 g/L glucose, in Dulbecco PBS | 23842900 |
Structure information of aptamer
| Aptamer Sequence: | ATACCAGCTTATTCAATTCCCAAATTGCCACCACTTACAGCATGATAACATACTACATCTTTTCATCAAGATAGTAAGTGCAATCT |
| The optimal secondary structure in dot-bracket notation: | .......................(((((....((((((((.(((....))).))..(((((......))))).))))))))))).. |
| The centroid secondary structure in dot-bracket notation: | ........................(((.....((((((((.(((....))).))..(((((......))))).)))))).)))... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_792 | Description | Aptamer N55 |
| Aptamer chemistry | DNA | Length | 86 nt |
| GC content | 34.9% | Molecular weight | 26,238.91 Da |
| Molarity of 1 μg/μl solution | 38.11 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Target recognition |
| Sequence | ATACCAGCTTATTCAATTCCCAAATTGCCACCACTTACAGCATGATAACATACTACATCTTTTCATCAAGATAGTAAGTGCAATCT | ||
| Applications | Recognize inflamed human aortic endothelial cells | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| ATCC ID | PCS-100-011 | Cell name | Inflamed human aortic endothelial cell |
| Category | Human cells | Organism | Homo sapiens, human |
| Product type | Primary cell | Cell type | endothelial cell |
| Morphology | Cobblestone appearance with large dark nuclei; during proliferation, cells are small and evenly sized, display a high mitotic index and show no presence of smooth muscle cells | Tissue | Heart; Aorta |
| Disease | Normal | Product format | Frozen |
| Antigen expression | On Willebrand Factor VIII positive and smooth muscle α-actin negative | Genes expressed | N/A |
| Cell description | Normal Primary Human Aortic Endothelial Cells, when grown in Vascular Cell Basal Media supplemented with Endothelial Cell Growth Kit components, provide an ideal cell system to propagate aortic endothelial cells in low serum conditions with or without the addition of human recombinant VEGF. |
|
|
| Application(s) | 3D cell culture; Cardiovascular disease research | ||
Activity data
 |
Interaction ID | 876 |
| Target type | Cell | |
| Target unique ID | PCS-100-011 | |
| Activity | 82 nM | |
| Binding Conditions/Buffer | 5 mmol/L MgCl2, 4.5 g/L glucose, in Dulbecco PBS |
|
| Assay | Dissociation plot for aptamer N55 binding to inflamed HAE cells |
|
| PubMed ID | 23842900 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_450 | DNA | ATACCAGCTTATTCAATTACATGTCCTGAAGGGGAATAATATACAGCTTTGGGTGGGTAGATAGTAAGTGCAATCT | |
| Apta_884 | DNA | ATACCAGCTTATTCAATTTGCACCACTTATTGTTACTAATCTGAGATAGTAAGTGCAATCT | |
| Apta_628 | DNA | ATACCAGCTTATTCAATTAGCCCGGTATTGAGGTCGATCTCTTATCCTATGGCTTGTCCCCCATGGCTCGGTTATATCCAGATAGTAAGTGCAATCT | |
| Apta_628 | DNA | ATACCAGCTTATTCAATTAGCCCGGTATTGAGGTCGATCTCTTATCCTATGGCTTGTCCCCCATGGCTCGGTTATATCCAGATAGTAAGTGCAATCT | |
| Apta_349 | DNA | ATACCAGCTTATTCAATTAGACGAGTTTTATTCATTTTCCATGTCGTAGCTTACCGTGAGATAGTAAGTGCAATCT | |
| Apta_452 | DNA | ATACCAGCTTATTCAATTGAAGAGTATAAAAAGAGTGATTATCTTTTGTAGGTTTTTTAGATAGTAAGTGCAATCT | |
| Apta_1016 | DNA | ATACCAGCTTATTCAATTTCTTCGCCAGTGCCAGGATCTCAGTTGGCGGTTCATTAGCTGGGTTGGTCGAAGATAGTAAGTGCAATCT | |
| Apta_451 | DNA | ATACCAGCTTATTCAATTGAGTAAGAGTGCACTATGTTTTACGAGCCGTTTCCTCGGCAGATAGTAAGTGCAATCT | |
| Apta_626 | DNA | ATACCAGCTTATTCAATTGGTGGTAGGATGGGCTCGATCGAGGTCTTCAGTCGGCTCGGTTTAGTTTATGTTCTCCTGTAGATAGTAAGTGCAATCT | |
| Apta_627 | DNA | ATACCAGCTTATTCAATTATGCGGGGATGAGGCTTGATCTGTGGCTCTGATCCATGATCAGTCGTCTTACGTGCGGTCCAGATAGTAAGTGCAATCT | |
| Apta_626 | DNA | ATACCAGCTTATTCAATTGGTGGTAGGATGGGCTCGATCGAGGTCTTCAGTCGGCTCGGTTTAGTTTATGTTCTCCTGTAGATAGTAAGTGCAATCT | |
| Apta_627 | DNA | ATACCAGCTTATTCAATTATGCGGGGATGAGGCTTGATCTGTGGCTCTGATCCATGATCAGTCGTCTTACGTGCGGTCCAGATAGTAAGTGCAATCT | |
| Apta_727 | DNA | ATACCAGCTTATTCAATTAGTCACACTTAGAGTTCTAACTGCTGCGCCGCCGGGAAAATACTGTACGGTTAGATAGTAAGTGCAATCT | |
| Apta_823 | DNA | GGGATACCAGCTTATTCAATTTGATTCCATCTTCCTGGACTGTCGAAAATTCAGTATCGGGAGGTTACGTATTTGGTTTATAGATAGTAAGTGCAATCT | |
| Apta_1015 | DNA | ATACCAGCTTATTCAATTGACCCGTTTCGTTCCCTCTGGGAAGTTTAGCCCAGTTGCCTGGGCGATACCAAGATAGTAAGTGCAATCT | |
| Apta_354 | DNA | ATACCAGCTTATTCAATTCTATACTCCACTTTGCTATTTCTCGGTTCCTTCACGCGCCGATCGCAGGCTGATGAATTGAGATAGTAAGTGCAATCT | |
| Apta_625 | DNA | ATACCAGCTTATTCAATTCAGGGCGGTATGAGGCTCGATCAAGGTCGGAGCGAGAATTTTTTCGCGGAGTCGGCTGGATCAGATAGTAAGTGCAATCT | |
| Apta_625 | DNA | ATACCAGCTTATTCAATTCAGGGCGGTATGAGGCTCGATCAAGGTCGGAGCGAGAATTTTTTCGCGGAGTCGGCTGGATCAGATAGTAAGTGCAATCT | |
| Apta_582 | DNA | GTACCAGCTTATTCAATTGGGCGAAGGAACATACGGCAGTTTATGGCCGCTATCGAGATAGACTATCATCTCAACGTCTTCTAGATAGTATGTTCATCAG | |
| Apta_805 | DNA | ATACCAGCTTATTCAATTAGGCGGTGCATTGTGGTTGGTAGTATACATGAGGTTTGGTTGAGACTAGTCGCAAGATATAGATAGTAAGTGCAATCT |
DEV TOOL
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