Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 943
Aptamer sequence: GCTATGGGTGGTCTGGTTGGGATTGGCCCCGGGAGCTGGC
Target unique ID: Other-6
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_857 | 6H7 | Other | His-Tagged Proteins | 4.6 nM | 50 mM K2HPO4, 150 mM NaCl, 0.05% Tween 20 (pH 7.5) | 21538335 |
Structure information of aptamer
| Aptamer Sequence: | GCTATGGGTGGTCTGGTTGGGATTGGCCCCGGGAGCTGGC |
| The optimal secondary structure in dot-bracket notation: | (((..((((.((((.....))))..))))....))).... |
| The centroid secondary structure in dot-bracket notation: | (((.......((((.....))))..........))).... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_857 | Description | 6H7 |
| Aptamer chemistry | DNA | Length | 40 nt |
| GC content | 67.5% | Molecular weight | 12,487.96 Da |
| Molarity of 1 μg/μl solution | 80.08 μM | Number of G-quadruplexes | 2 |
| G-Score | 21 | Function | Targeted binding |
| Sequence | GCTATGGGTGGTCTGGTTGGGATTGGCCCCGGGAGCTGGC | ||
| Applications | Purification of different His-tagged proteins | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Target ID | Other-6 | Name | His-Tagged Proteins |
Activity data
 |
Interaction ID | 943 |
| Target type | Other | |
| Target unique ID | Other-6 | |
| Activity | 4.6 nM | |
| Binding Conditions/Buffer | 50 mM K2HPO4, 150 mM NaCl, 0.05% Tween 20 (pH 7.5) |
|
| Assay | Variation of imidazole concentration used for protein elution. Imidazole concentrations below 1 M result in insufficient elution |
|
| PubMed ID | 21538335 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_858 | DNA | GGCTTCAGGTTGGTCTGGTTGGGTTTGGCTCCTGTGTACG | |
| Apta_861 | DNA | CAGGTTGGTCTGGTTGGGTTTGGCTCCTGTGTACGCAACCTG | |
| Apta_1205 | DNA | GGCAGGTGGTGTTGGTTGGTTGTGCGTGGAGTTGG | |
| Apta_25 | DNA | TGTGGGGGTGGTTGGGCGGGGCTGC | |
| Apta_749 | DNA | GATCGGTGGGTGGGGGGGTTGGAGATCATCCTCAGGGATTACGTC | |
| Apta_650 | DNA | GCACACCGATGGCGGTCCTGTTTAGGGGGTGTGCGTGGGGAGGTGCGGTTGC | |
| Apta_18 | DNA | TGGGGGGTTGGACGGGCGGGGCTGC | |
| Apta_24 | DNA | TGTGGGGATGGATGGGCCGGGCTGC | |
| Apta_41 | DNA | CGGTCGCTCCGTGTGGCTTGGGTTGGGTGTGGCAGTGAC | |
| Apta_75 | DNA | CACTGGTAGGTTGGTGTGGTTGGGGCCAGTG | |
| Apta_263 | DNA | AGAGGGGAGGGTCGGGTATCGGCGTGTTCGGGGGATCTGC | |
| Apta_1046 | DNA | TCGCATGGAGGGCAGAGCCGCCTGCCGGGATCCGGCCCTCTTGGGCGGGC | |
| Apta_255 | DNA | TAGCGATACTGCGTGGGTTGGGGCGGGTAGGGCCAGCAGTCTCGT | |
| Apta_10 | DNA | GGGGGGGGTGGCTGGGCGGGGTGGG | |
| Apta_13 | DNA | AGGGGGGGGGGAGGGGTCGGGGTGG | |
| Apta_15 | DNA | TGAGGGGGGGGGAGGGCGGGGCTGC | |
| Apta_42 | DNA | GCTGGTCTCTGCGGGTTGTTGCGCCGCGGCACCCTTGGCA | |
| Apta_124 | DNA | GGTGGTGGGGGGGGTTGGTAGGGTGTCTTC | |
| Apta_148 | DNA | CCGTAGGTTCGGGGTCGGAGTGGTCCGGAAGGTGGCGTGG | |
| Apta_149 | DNA | CCGTAGGTTCGGGGTCGGAGTGGTCCGGAAGATGGCGTGG |
DEV TOOL
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