Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1170
Aptamer sequence: TAATACGACTCACTATAGGGAGACCCAAGCCGATTTATTTTGTGCAGCTTTTGTTCCCTTTAGTGAGGGTTAATT3
Target unique ID: P03129
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_1114 G5α3N.4 Protein VE7_HPV16 1.9 μM 0.5 M ammonium acetate, 1 mM EDTA, 0.1% SDS and 20 U RNaseA inhibitor 21565620
Structure information of aptamer
Aptamer Sequence: TAATACGACTCACTATAGGGAGACCCAAGCCGATTTATTTTGTGCAGCTTTTGTTCCCTTTAGTGAGGGTTAATT3
The optimal secondary structure in dot-bracket notation: ........(((((((.((((((....(((((((......)))....))))...)))))).)))))))........
The centroid secondary structure in dot-bracket notation: ........(((((((.((((((.(..(((((((......)))....))))..))))))).)))))))........
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_1114 Description G5α3N.4
Aptamer chemistry DNA Length 75 nt
GC content 40.0% Molecular weight 23,104.82 Da
Molarity of 1 μg/μl solution 43.28 μM Number of G-quadruplexes No QGRS found
G-Score N/A Function Target detection
Sequence TAATACGACTCACTATAGGGAGACCCAAGCCGATTTATTTTGTGCAGCTTTTGTTCCCTTTAGTGAGGGTTAATT3
Applications Detection of papillomavirus infection and cervical cancer
Target information
Type Detail Type Detail
Uniprot ID P03129 Protein name Protein E7
Gene name(s) E7 Organism Protein E7
Status reviewed in uniprot database Length 98
Mass 11,022 Activity regulation N/A
Involvement in disease N/A Catalytic activity N/A
PDB ID(s)
4YOZ;6APN;
Function Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle.
Activity data
Interaction ID 1170
Target type Protein
Target unique ID P03129
Activity 1.9 μM
Binding Conditions/Buffer

0.5 M ammonium acetate, 1 mM EDTA, 0.1% SDS and 20 U RNaseA inhibitor
Assay

Hill plot showing the KD for the G5a3N.4 interaction with purified HPV-16 E7 protein
PubMed ID 21565620
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_281 DNA CCGTAATACGACTCACTATAGGGGAGCTCGGTACCGAATTCATGTTGGTAGTTGGCTTGTGTTCGTGTGTTAAGCTTTCAGAGAGGATCCTT 63.04%
Apta_1103 DNA TACGACTCACTATAGGGATCCACCTATGGCAGATTGAGCCCAAGGGCTGTGCAGCGAATTCCCTTTAGTGAGGGTT 59.21%
Apta_550 DNA GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATTTTACAGAACAACCAACGTCGCTCCGGGTACTTCTTCATCGAGATAGTAAGTGCAATCT 58.00%
Apta_1102 DNA TACGACTCACTATAGGGATCCGTGACTGTACGGGCTCAGTCGTTACTTGAGAGTTGAATTCCCTTTAGTGAGGGTT 57.89%
Apta_1105 DNA TACGACTCACTATAGGGATCCAATGGCACAGCGCCTGGAACGTACTCTGTACCTGGAATTCCCTTTAGTGAGGGTT 57.89%
Apta_1104 DNA TACGACTCACTATAGGGATCCCCCCGAGTGAAGAGCAGGACAGCGGGACAGCGTCGAATTCCCTTTAGTGAGGGTT 56.58%
Apta_1106 DNA TACGACTCACTATAGGGATCCTTCGCATCCGGCACGATGGCTAGGACACCCCGATGAATTCCCTTTAGTGAGGGTT 56.58%
Apta_932 DNA ATACGACTCACTATTAGGGATGTCCACCATATAGATCGATTTAAGTCCCTCGTTATTAATGCTAGACTACTGACTACAA 54.43%
Apta_1101 DNA CCGTAATACGACTCACTATAGGTATGTGACGCGCGTCAATCGCTGTCCTACCAAGCTTTGCAGAGAGGATCCTT 53.95%
Apta_944 DNA ATACGACTCACTATTAGGGACAAAGCTGACAACCCTTTCATAATCTAACTACATTTATGTGCTAGACTACTGACTAC 53.25%
Apta_452 DNA ATACCAGCTTATTCAATTGAAGAGTATAAAAAGAGTGATTATCTTTTGTAGGTTTTTTAGATAGTAAGTGCAATCT 52.63%
Apta_953 DNA TCACCTCATACGACTCACTATAGCGGATCCGAGCCGGGGTGTTCTGTTGGCGGGGGCGGTGGGCGGGCTGGCTCGAACAAGCTTGC 52.33%
Apta_626 DNA ATACCAGCTTATTCAATTGGTGGTAGGATGGGCTCGATCGAGGTCTTCAGTCGGCTCGGTTTAGTTTATGTTCTCCTGTAGATAGTAAGTGCAATCT 51.55%
Apta_804 DNA GCGGGATCCTATGACGCATTGACCCACAACACACTACTGTCGCTCGGTTCGAACTTCGTGCGACTGTTCCCTATAGTGAGTCGTATTAGAATTCCGC 51.55%
Apta_626 DNA ATACCAGCTTATTCAATTGGTGGTAGGATGGGCTCGATCGAGGTCTTCAGTCGGCTCGGTTTAGTTTATGTTCTCCTGTAGATAGTAAGTGCAATCT 51.55%
Apta_993 DNA AATCCGACCACGAGAGCAGACCGACCACAGCATTATCTAAACCCATTAGTCTAGTCCTGG 51.32%
Apta_950 DNA CACCTAATACGACTCACTATAGCGGATCCGAAGGGGGCGCGAGGTGTAAGGGTGTGGGGTGGTGGGTGGGCCTGGCTCGAACAAGCTTGC 51.11%
Apta_931 DNA ATACGACTCACTATTAGGGACCACATAAACATGCATCGCTCAATAACGGGATATTGTTGTGCTAGACTACTGACTACAA 50.63%
Apta_736 DNA ATCGTCTGCTCCGTCCAATATGATGACACCTGCATAATTTATAGTGAGTCTTGATTCACGCTGCATTTGGTGTGAGGTCGTGC 50.60%
Apta_1174 DNA ATGAGAGCGTCGGTGTGGTAAGTATGATATCGGTGTTTATGGTGTCTGTCTTCATACTCTTGCTGTAGGAGGGTGCGGAAGTA 50.60%

ABOUT

AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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