Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1106
Aptamer sequence: CTTCTGCCCGCCTCCTTCCCGGGTTACAGGATCGTAAATCTTATTCGTTGCTCGATCGGGAGACGAGATAGGCGGACACT
Target unique ID: P02144
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_1030 M03 Protein MYG_HUMAN 46.3 nM Ammonium bicarbonate buffer (50 mM, pH 8.5) containing 500 mM NaCl 32631049
Structure information of aptamer
Aptamer Sequence: CTTCTGCCCGCCTCCTTCCCGGGTTACAGGATCGTAAATCTTATTCGTTGCTCGATCGGGAGACGAGATAGGCGGACACT
The optimal secondary structure in dot-bracket notation: ..((((((((.(((((...(((((.(((((((.....)))))....)).)))))...))))).)).....))))))....
The centroid secondary structure in dot-bracket notation: ..((((((...........(((((.((.(((.......))).....)).)))))................))))))....
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_1030 Description M03
Aptamer chemistry DNA Length 80 nt
GC content 55.0% Molecular weight 24,588.70 Da
Molarity of 1 μg/μl solution 40.67 μM Number of G-quadruplexes No QGRS found
G-Score N/A Function Target recognition
Sequence CTTCTGCCCGCCTCCTTCCCGGGTTACAGGATCGTAAATCTTATTCGTTGCTCGATCGGGAGACGAGATAGGCGGACACT
Applications Detecting Mb in human serum and distinguishing acute myocardial infarction patients from healthy individuals
Target information
Type Detail Type Detail
Uniprot ID P02144 Protein name Myoglobin
Gene name(s) MB Organism Myoglobin
Status reviewed in uniprot database Length 154
Mass 17,184 Activity regulation N/A
Involvement in disease N/A Catalytic activity N/A
PDB ID(s)
3RGK;
Function Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
Activity data
Interaction ID 1106
Target type Protein
Target unique ID P02144
Activity 46.3 nM
Binding Conditions/Buffer

Ammonium bicarbonate buffer (50 mM, pH 8.5) containing 500 mM NaCl
Assay

Characterization of selected Mb-binding aptamers
PubMed ID 32631049
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_1029 DNA CTTCTGCCCGCCTCCTTCCCGGTCGAGACCCGGGTTTACTTCCTCGGTAGTGTTCGAGGGAGACGAGATAGGCGGACACT 75.00%
Apta_1028 DNA CTTCTGCCCGCCTCCTTCCGGCGTCCCTGCGCACTGTATAACGTACACACGATACGTGGGAGACGAGATAGGCGGACACT 73.75%
Apta_1074 DNA CTTCTGCCCGCCTCCTTCCGCCACAGTATTATGTATCACAGTGCTTCATCTAATATGAGGAGACGAGATAGGCGGACACT 72.50%
Apta_1073 DNA CTTCTGCCCGCCTCCTTCCTATTAGGGTGCTTCGCGAAGTTCATTTTACATCCCCATAGGAGACGAGATAGGCGGACACT 71.25%
Apta_1075 DNA CTTCTGCCCGCCTCCTTCCTACTAGGGTGCTTCGCGAAGTTCATTTTACATCCCCATAGGAGACGAGATAGGCGGACACT 71.25%
Apta_1076 DNA CTTCTGCCCGCCTCCTTCCTATTAGGGTGCTTCGCGAAGTTCATTTTACACCCCCATAGGAGACGAGATAGGCGGACACT 70.00%
Apta_737 DNA ATCGTCTGCTCCGTCCAATAGTGCATTGAAACTTCTGCATCCTCGTTTGGTGTGAGGTCGTGC 52.50%
Apta_884 DNA ATACCAGCTTATTCAATTTGCACCACTTATTGTTACTAATCTGAGATAGTAAGTGCAATCT 52.50%
Apta_903 DNA ATACGGGAGCCAACACCAGACGGTAAATAAAGTCAGTATCGATACTCTATTCGAGAGCAGGTGTGACGGAT 52.50%
Apta_1049 DNA ATACGGGAGCCAACACCATGTACATTGTAGATTTATTTGCCCAAAACCCAAGAAGAGCAGGTGTGACGGAT 52.50%
Apta_1016 DNA ATACCAGCTTATTCAATTTCTTCGCCAGTGCCAGGATCTCAGTTGGCGGTTCATTAGCTGGGTTGGTCGAAGATAGTAAGTGCAATCT 52.27%
Apta_628 DNA ATACCAGCTTATTCAATTAGCCCGGTATTGAGGTCGATCTCTTATCCTATGGCTTGTCCCCCATGGCTCGGTTATATCCAGATAGTAAGTGCAATCT 51.55%
Apta_628 DNA ATACCAGCTTATTCAATTAGCCCGGTATTGAGGTCGATCTCTTATCCTATGGCTTGTCCCCCATGGCTCGGTTATATCCAGATAGTAAGTGCAATCT 51.55%
Apta_349 DNA ATACCAGCTTATTCAATTAGACGAGTTTTATTCATTTTCCATGTCGTAGCTTACCGTGAGATAGTAAGTGCAATCT 51.25%
Apta_536 DNA ATCCGTCACACCTGCTCTCGAATAGAGTACTCGATACTGACTTTATTTACCGTCTGGTGTTGGCTCCCGTAT 51.25%
Apta_899 DNA ATCCGTCACACCTGCTCTCGAATAGAGTATTCGATACTGACTTTATTTACCGTCTGGTGTTGGCTCCCGTAT 51.25%
Apta_904 DNA ATACGGGAGCCAACACCAGACGGTAAATAAAGTCAGTATCGAGTACTCTATTCGAGAGCAGGTGTGACGGAT 51.25%
Apta_1279 DNA TTCCAAGACCTGACCGTAATCGTCAAATGTGTTATTGGAGGGGGACTAGCCCCCGGAGGG 51.25%
Apta_933 DNA GACGCTTACTCAGGTGTGACTCGGGGTGTAGCTCGTTATGATTCGGACAAGACTTACCTTGCGCCTCTGGGATCGAAGGACGCAGATGAAGTCTC 50.53%
Apta_281 DNA CCGTAATACGACTCACTATAGGGGAGCTCGGTACCGAATTCATGTTGGTAGTTGGCTTGTGTTCGTGTGTTAAGCTTTCAGAGAGGATCCTT 50.00%

ABOUT

AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

HELP CENTER

>Contact us
>LMMD Group
>School of Pharmacy

DEV TOOL
Page last updated at 2022-05-25 10:01:57 (Asia/Shanghai) | You are visitor No. 43983
Copyright © 2019-2020 Laboratory of Molecular Modeling and Design, School of Pharmacy, East China University of Science and Technology. All rights reserved.