AptaDB: Result

Interaction ID: 1103

Aptamer sequence: GGAGCTACGTTTAGCGAGTCCGACGCTCGGTGCCTCTTC

Target unique ID: Other-48

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_1027	Apta 5	Other	Carcinoembryonic antigen (CEA)	37.8 nM	N/A	32527457

Aptamer Sequence:	GGAGCTACGTTTAGCGAGTCCGACGCTCGGTGCCTCTTC
The optimal secondary structure in dot-bracket notation:	...((((....))))(((.((((...))))...)))...
The centroid secondary structure in dot-bracket notation:	.......................................

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_1027	Description	Apta 5
Aptamer chemistry	DNA	Length	39 nt
GC content	61.5%	Molecular weight	11,966.66 Da
Molarity of 1 μg/μl solution	83.57 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Target recognition
Sequence	GGAGCTACGTTTAGCGAGTCCGACGCTCGGTGCCTCTTC
Applications	Specific to CEA for use as radiopharmaceuticals in colorectal cancer diagnosis

Type	Detail	Type	Detail
Target ID	Other-48	Name	Carcinoembryonic antigen (CEA)

	Interaction ID	1103
	Target type	Other
	Target unique ID	Other-48
	Activity	37.8 nM
	Binding Conditions/Buffer	N/A
	Assay	Binding saturation curves of aptamers incubated with T84 cell line. Kd was calculated using nonlinear regression with Specific Binding equation
	PubMed ID	32527457

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_229	DNA	GGGGCACGTTTATCCGTCCCTCCTAGTGGCGTGCCCC	61.54%
Apta_1242	DNA	GGAGCGATTAGTAGTGGTCTTGTCTTTTGC	58.97%
Apta_1146	DNA	GGGACAGGGAGTGCGCTGCTCCCC	56.41%
Apta_914	DNA	CGCTCCGTTTACTCGGTCTGCTATTGGCTTGCCTTTGTTT	55.00%
Apta_963	DNA	TGGCGCTGCCCCTAGTCTCCGGCTGATAGCTGCTTCTTGG	55.00%
Apta_71	DNA	GGCGAGTAGGTTGGTGTGGTTGGGGCTCGCC	53.85%
Apta_73	DNA	GGGGAGTAGGTTGGTGTGGTTGGGGCTCCCC	53.85%
Apta_74	DNA	GGACAGTAGGTTGGTGTGGTTGGGGCTGTCC	53.85%
Apta_249	DNA	CCCTGTTCCCCCTCCGCCGCTTGACTCTCC	53.85%
Apta_358	DNA	GGGAATCGCCACCCGACGCAGGGTTTCCC	53.85%
Apta_392	DNA	GGATAGTTCGGTGGCTGTTCAGGGTCTCCTCCT	53.85%
Apta_646	DNA	TGGAGCTTGGATTGATGTGGTGTGTGAGTGCGGTGCCC	53.85%
Apta_1026	DNA	GGGGGGTGTATCGTTGACGAGTTGCGCGTGCGTCTCGTG	53.85%
Apta_1204	DNA	GGCACGATGTGGCTACATCGATCGCGGTACTGGTG	53.85%
Apta_1204	DNA	GGCACGATGTGGCTACATCGATCGCGGTACTGGTG	53.85%
Apta_1246	DNA	GGAGATGTATGAGCGGGTGTTTGTGTTTGC	53.85%
Apta_1125	DNA	TCGGGCGAGTCGTCTGGGGAGGTCAGAACGAAAGGCCCGCATCGTCCTCCC	52.94%
Apta_1127	DNA	TCGGGCGAGTCGTCTGCGAGGCAACAAGCTCGTAATCCGCATCGTCCTCCC	52.94%
Apta_1219	DNA	GCGCAGAACCGACAGCAAAACGGAAGTCCACCACGACTATTGGTGCCCAATCTACCGTA	52.54%
Apta_7	DNA	CCAGTCTCCCGTTTACCGCGCCTACACATGTCTGAATGCC	52.50%

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