Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1174
Aptamer sequence: GGGAATGGATCCACATCTACGAATTCATCAGGGCTAAAGAGTGCAGAGTTACTTAGTTCACTGCAGACTTGACGAAGCTT
Target unique ID: P83673
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_1118 clone 1 Protein LYS1_CRAVI 29 nM 20 mM Tris pH 7.6, 100 mM NaCl, 5 mM MgCl2 15253644
Structure information of aptamer
Aptamer Sequence: GGGAATGGATCCACATCTACGAATTCATCAGGGCTAAAGAGTGCAGAGTTACTTAGTTCACTGCAGACTTGACGAAGCTT
The optimal secondary structure in dot-bracket notation: .(((.....)))...........(((.(((((.........(((((..............)))))..))))).)))....
The centroid secondary structure in dot-bracket notation: ...............................(((.......(((((..............)))))...........))).
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_1118 Description clone 1
Aptamer chemistry DNA Length 80 nt
GC content 45.0% Molecular weight 24,749.89 Da
Molarity of 1 μg/μl solution 40.40 μM Number of G-quadruplexes No QGRS found
G-Score N/A Function Target detection
Sequence GGGAATGGATCCACATCTACGAATTCATCAGGGCTAAAGAGTGCAGAGTTACTTAGTTCACTGCAGACTTGACGAAGCTT
Applications Detection and Quantitation of Proteins
Target information
Type Detail Type Detail
Uniprot ID P83673 Protein name Lysozyme 1 (EC 3.2.1.17) (1,4-beta-N-acetylmuramidase 1) (Invertebrate-type lysozyme 1) (cv-lysozyme 1)
Gene name(s) lysoz1 lysoz Organism Lysozyme 1 (EC 3.2.1.17) (1,4-beta-N-acetylmuramidase 1) (Invertebrate-type lysozyme 1) (cv-lysozyme 1)
Status reviewed in uniprot database Length 184
Mass 19,986 Activity regulation N/A
Involvement in disease N/A Catalytic activity Reaction=Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
PDB ID(s)
N/A
Function Has antibacterial activity against the Gram-positive bacteria L.garvieae, M.luteus and Enterococcus sp.
Activity data
Interaction ID 1174
Target type Protein
Target unique ID P83673
Activity 29 nM
Binding Conditions/Buffer

20 mM Tris pH 7.6, 100 mM NaCl, 5 mM MgCl2
Assay

Fluorescence data showing the reactivity of the three anti-lysozyme aptamer clones after 10 min of washing
PubMed ID 15253644
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_495 DNA GGGAATGGATCCACATCTACGTATTACTGCTGGTGACGAGGGCTAGACGACGTACCTTCACTGCAGACTTGACGAAGCTT 78.75%
Apta_1119 DNA GGGAATGGATCCACATCTACGAATTCGGTTGTGAAGATTGGGAGCGTCGTGGCTACTTCACTGCAGACTTGACGAAGCTT 77.50%
Apta_1120 DNA GGGAATGGATCCACATCTACGAATTCGGAATGAGTGCCCTGCAAGCGAGGGCTAGCTTCACTGCAGACTTGACGAAGCTT 77.50%
Apta_587 DNA GGAATGGATCCACATCCATGGATGGGCAATGCGGGGTGGAGAATGGTTGCCGCACTTCGGCTTCACTGCAGACTTGACGAAGCTT 71.76%
Apta_421 DNA ATCTACGAATTCATCAGGGCTAAAGAGTGCAGAGTTACTTAGCCCGTGA 61.25%
Apta_623 RNA GGGAAUGGAUCCACAUCUACGAAUUCACCGCGGGGUUGCGGACCGGGAGCUCCAGCUUCACUGCAGACUUGACGAAGCUU 60.00%
Apta_589 DNA GGGAATTCGAGCTCGGTACCATCTGTGTAAGGGGTAAGGGGTGGGGGTGGGTACGTCTAGCTGCAGGCATGCAAGCTTGG 56.25%
Apta_624 RNA GGGAAUGGAUCCACAUCUACGAAUUCGAAUUGCCGUAAUUUCCCGUGGAGCGAUGCUUCACUGCAGACUUGACGAAGCUU 56.25%
Apta_589 DNA GGGAATTCGAGCTCGGTACCATCTGTGTAAGGGGTAAGGGGTGGGGGTGGGTACGTCTAGCTGCAGGCATGCAAGCTTGG 56.25%
Apta_531 DNA ATACGGGAGCCAACACCACATCGACTTACGAATCAACGCGTTTATTATTGGTTCAGAGCAGGTGTGACGGAT 53.75%
Apta_904 DNA ATACGGGAGCCAACACCAGACGGTAAATAAAGTCAGTATCGAGTACTCTATTCGAGAGCAGGTGTGACGGAT 53.75%
Apta_811 DNA ATAGGAGTCACGACGACCAGAATCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACTTATGTGCGTCTACCTCTTGACTAAT 52.87%
Apta_355 DNA GATGCAGCCACCTACATTGCAGGTTTTATAATACAACTACGCGCCGTTCGCAGGCTAGTT 52.50%
Apta_501 DNA GGGAGACAAGAATAAACGCTCAATTTAACGTATTTATTCAAGCTCGTATTCGACAGGAGGCTCACAACAGGC 52.50%
Apta_684 DNA GGCGGACCAAGGGGACACCACAGATGAATGTACAGTACCATGTTACTGCGCCCGTAGGTG 52.50%
Apta_810 DNA ACAGCATCCCCATGTGAACAATCGCATTGTGATTGTTACGGTTTCCGCCTCATGGACGTGCTG 52.50%
Apta_824 DNA ATACGGGAGCCAACACCATCCCTCTTAGGATACAAAGCCAAACTGAGCCCGTGCAGAGCAGGTGTGACGGAT 52.50%
Apta_902 DNA ATACGGGAGCCAACACCACCAACATTATCAGAGTATGTTACTTATAGTGGTGGCAGAGCAGGTGTGACGGAT 52.50%
Apta_903 DNA ATACGGGAGCCAACACCAGACGGTAAATAAAGTCAGTATCGATACTCTATTCGAGAGCAGGTGTGACGGAT 52.50%
Apta_954 DNA ATACGGGAGCCAACACCAGGACTAACATTATAAGAATTGCGAATAATCATTGGAGAGCAGGTGTGACGGAT 52.50%

ABOUT

AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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