Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 944
Aptamer sequence: GGCTTCAGGTTGGTCTGGTTGGGTTTGGCTCCTGTGTACG
Target unique ID: Other-6
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_858 | 6H5 | Other | His-Tagged Proteins | 9.7 nM | 50 mM K2HPO4, 150 mM NaCl, 0.05% Tween 20 (pH 7.5) | 21538335 |
Structure information of aptamer
| Aptamer Sequence: | GGCTTCAGGTTGGTCTGGTTGGGTTTGGCTCCTGTGTACG |
| The optimal secondary structure in dot-bracket notation: | .((..((((..((((...........)))))))).))... |
| The centroid secondary structure in dot-bracket notation: | .((..((((..((((...........)))))))).))... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_858 | Description | 6H5 |
| Aptamer chemistry | DNA | Length | 40 nt |
| GC content | 57.5% | Molecular weight | 12,418.92 Da |
| Molarity of 1 μg/μl solution | 80.52 μM | Number of G-quadruplexes | 2 |
| G-Score | 21 | Function | Targeted binding |
| Sequence | GGCTTCAGGTTGGTCTGGTTGGGTTTGGCTCCTGTGTACG | ||
| Applications | Purification of different His-tagged proteins | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Target ID | Other-6 | Name | His-Tagged Proteins |
Activity data
 |
Interaction ID | 944 |
| Target type | Other | |
| Target unique ID | Other-6 | |
| Activity | 9.7 nM | |
| Binding Conditions/Buffer | 50 mM K2HPO4, 150 mM NaCl, 0.05% Tween 20 (pH 7.5) |
|
| Assay | Variation of imidazole concentration used for protein elution. Imidazole concentrations below 1 M result in insufficient elution |
|
| PubMed ID | 21538335 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_861 | DNA | CAGGTTGGTCTGGTTGGGTTTGGCTCCTGTGTACGCAACCTG | |
| Apta_857 | DNA | GCTATGGGTGGTCTGGTTGGGATTGGCCCCGGGAGCTGGC | |
| Apta_1237 | DNA | GGGGTTGTGTGGGGAGTTTGTCCTGTTGTG | |
| Apta_71 | DNA | GGCGAGTAGGTTGGTGTGGTTGGGGCTCGCC | |
| Apta_72 | DNA | AGTCAGTAGGTTGGTGTGGTTGGGGCTGACT | |
| Apta_74 | DNA | GGACAGTAGGTTGGTGTGGTTGGGGCTGTCC | |
| Apta_146 | DNA | GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCG | |
| Apta_1205 | DNA | GGCAGGTGGTGTTGGTTGGTTGTGCGTGGAGTTGG | |
| Apta_1247 | DNA | GGTGGTTGCTGTTGTTTGTGGTTGGTGTGC | |
| Apta_41 | DNA | CGGTCGCTCCGTGTGGCTTGGGTTGGGTGTGGCAGTGAC | |
| Apta_69 | DNA | AGCGAGTAGGTTGGTGTGGTTGGGGCTCGCT | |
| Apta_73 | DNA | GGGGAGTAGGTTGGTGTGGTTGGGGCTCCCC | |
| Apta_75 | DNA | CACTGGTAGGTTGGTGTGGTTGGGGCCAGTG | |
| Apta_350 | DNA | GGTGGTGGTGGTTGTGGTGGTGGTGG | |
| Apta_482 | DNA | AGCAAGGTTTGGTGTTGGTTGGTTGCTGGTTTTGGTTTC | |
| Apta_682 | DNA | CCTTGTAGATCGGGTGTGGTTTGGCGTAGG | |
| Apta_914 | DNA | CGCTCCGTTTACTCGGTCTGCTATTGGCTTGCCTTTGTTT | |
| Apta_1085 | DNA | TGGGGTGGTGGTGTGGGTGGGGGTTTGTTC | |
| Apta_1243 | DNA | GGTGTTGTTGTTGGCTAGTTTGTTGTGGGC | |
| Apta_144 | DNA | GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCGATAGAGGTAG |
DEV TOOL
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