Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 877
Aptamer sequence: GGGAGAGAACAAUGACCUGCGGUGCCAAGCCGUCGGGUUAUGUUGAUCUCCUCAAGGACGAGUGCAUUGCAUCACGUCAGUAG
Target unique ID: HTB-177
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_793 | RA16 | Cell | NCI-H460 non-small-cell lung cancer cells | 9 nM | 400 mM Tris-HCl, 80 mM MgCl2, and 20 mM spermidine | 29499932 |
Structure information of aptamer
| Aptamer Sequence: | GGGAGAGAACAAUGACCUGCGGUGCCAAGCCGUCGGGUUAUGUUGAUCUCCUCAAGGACGAGUGCAUUGCAUCACGUCAGUAG |
| The optimal secondary structure in dot-bracket notation: | ((((((.((((.(((((((((((.....)))).)))))))))))..))))))....((((.((((...))))..))))..... |
| The centroid secondary structure in dot-bracket notation: | ((((((.(((.((((((((((((.....)))).)))))))))))..))))))....((((.((((...))))..))))..... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_793 | Description | RA16 |
| Aptamer chemistry | RNA | Length | 83 nt |
| GC content | 54.2% | Molecular weight | 26,806.93 Da |
| Molarity of 1 μg/μl solution | 37.30 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted therapy |
| Sequence | GGGAGAGAACAAUGACCUGCGGUGCCAAGCCGUCGGGUUAUGUUGAUCUCCUCAAGGACGAGUGCAUUGCAUCACGUCAGUAG | ||
| Applications | Inhibited cancer cell proliferation | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| ATCC ID | HTB-177 | Cell name | NCI-H460 non-small-cell lung cancer cells |
| Category | Human cells | Organism | Homo sapiens, human |
| Product type | N/A | Cell type | N/A |
| Morphology | epithelial | Tissue | Lung; Pleural effusion |
| Disease | Carcinoma | Product format | Frozen |
| Antigen expression | N/A | Genes expressed | N/A |
| Cell description | NCI-H460 [H460] cells were isolated in 1982 from the pleural fluid of a male patient with large cell lung cancer. Use these cells in your cancer and toxicology research. |
|
|
| Application(s) | 3D cell culture; Cancer research; High-throughput screening; Toxicology | ||
Activity data
 |
Interaction ID | 877 |
| Target type | Cell | |
| Target unique ID | HTB-177 | |
| Activity | 9 nM | |
| Binding Conditions/Buffer | 400 mM Tris-HCl, 80 mM MgCl2, and 20 mM spermidine |
|
| Assay | The binding curve of FITC-labeled RA16 (5–200 nM) and mean fluorescence intensities (MFIs) obtained by flow cytometry, KD = 9 ± 2 nM. Three independent experiments were performed; all data represent means ± SD, n = 3 |
|
| PubMed ID | 29499932 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_724 | RNA | GGGAGAGGAUACUACUGUCAUACGUUAGGCUGUAGGCGAGGUGAAAUGAGCGGUAAUAGCCUCAGCGUAGCAUAUGCAUGAAUUCGAAGCUUCGC | |
| Apta_39 | RNA | GGUAGAUACGAUGGACUACCGCCACCCCGGGUCCGGGUGACGUUAAUUGAGGGCCCAUGACGCGCAGCCA | |
| Apta_129 | RNA | GGGAGGACGAUGCGGACACCGUUAAUCUGAGGCCCUGUCCUAUUCCUUCACGCCUCAGA | |
| Apta_131 | RNA | GGGAGGACGAUGCGGUCCUGUCGUCUGUUCGUCCCCAGACGACUCGCCCGA | |
| Apta_1224 | RNA | GGAGGCGCCAACUGAAUGAAUGUUCUGGAUCUGCUAGAGACUAUCUGCUUCGGCAGCACUCUCCGUUAACAACACGUACAUCCGUAACUAGUCGCGUCAC | |
| Apta_433 | RNA | GGGAGACAAGAAUAAACGCUCAACGUUCAGUAUAACAGUCCGAGUCUAACACACAAUGGGACACUGAAUUCGACAGGAGGCUCACAACAGGC | |
| Apta_133 | RNA | GGGAGGACGAUGCGGUGAGUCGUUCCCUUCGUCCCCAGACGACUCGCCCGA | |
| Apta_134 | RNA | GGGAGGACGAUGCGGGCCGUUGUUGUGUGCCGCCCCAGACGACUCGCCCGA | |
| Apta_305 | RNA | GGGAGGACGAUGCGGAUCGCCCUGAACCGGCCCAGCAGACUGCUGACGGCACGAUCCGCAUCGUCCUCCC | |
| Apta_696 | RNA | GAGUGCGCCCAUUGAUAAGAGCGUAGGUUCAACGGAGUCGUAGUCGUUCUCAAGUUGACGGUGCAUUAGAGUCCU | |
| Apta_1221 | RNA | GGAGGCGCCAACUGAAUGAACGAGUUGAAAUGCGGAUCAAGUUACUGCUUCGGCAGUACCUAGUGGGAAAAGUUCUUGGGUCCGUAACUAGUCGCGUCAC | |
| Apta_1222 | RNA | GGAGGCGCCAACUGAAUGAAACCGUGCUAAGGAGUUGGACUCCGCUGCUUCGGCAGAGCGAUAUAGUGCCAGCUGAUACAUCCGUAACUAGUCGCGUCAC | |
| Apta_936 | RNA | GGAGCUCAGCCUUCACUGCGAUCACCGUGUCGGUGGCUAGUCGAUGUAUCCCGAUCACCAUUGGGGGCAAGUUUAGACAGGCACCACGGUCGGAUCCAC | |
| Apta_432 | RNA | GGGAGACAAGAAUAAACGCUCAAGGCAUUUCUGCUAGUCUGGUUGUAAGAUAUUCAACACGUGAGUUUCGACAGGAGGCUCACAACAGGC | |
| Apta_132 | RNA | GGGAGGACGAUGCGGCGUUUCCUCUGGUUCGUCCCCAGACGACUCGCCCGA | |
| Apta_520 | RNA | GGGAGAUCUACGGAUCUCAGGGCUCUUACGGGAGCUACAUGGAAGGAGUCCAUGUGU | |
| Apta_984 | RNA | GGGAGGACGAUGCGGCGUAUUGCGCGAGGAUUAUCCGCUCAUCGUUGUUGUUGUGCAGACGACUCGCCCGA | |
| Apta_1053 | RNA | GCCGCAAGUACGAGGGCGGCGCGCUCGACGCGGUUAGAUGAGUGUCGCCGUUUCCGAAGAUUGGCUACGCGGGG | |
| Apta_1168 | RNA | GCGACUACGGUGAGGGUCGGGUCCAGUAGCUUCGGCUACUGUUGAGUAGAGUGUGGGCUCCGUAGUCGC | |
| Apta_410 | RNA | GGGAGAGCGGAAGCGUGCUGGGCCUGUCGUUCGCUGUGUCUUGCUACGUUACGUUACACGGUUGGCAUAACCCAGAGGUCGAUGG |
DEV TOOL
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