Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 252
Aptamer sequence: GGCTTTACACTTTATGCTTCCGTACCAGAACATATCGAGTGATCCTGGGAGGATAACAATTTCACACAGGAAACAG
Target unique ID: P08246
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_247 | BnHNE2 | Protein | ELNE_HUMAN | 49.2 nM | 20 mM HEPES pH 7.5, 150 mM NaCl, 6 mM KCl, 2 mM MgCl2, room temperature | 31961667 |
Structure information of aptamer
| Aptamer Sequence: | GGCTTTACACTTTATGCTTCCGTACCAGAACATATCGAGTGATCCTGGGAGGATAACAATTTCACACAGGAAACAG |
| The optimal secondary structure in dot-bracket notation: | .......(((((((((.(((.......)))))))..))))).(((((....((........))...)))))..... |
| The centroid secondary structure in dot-bracket notation: | .......(((((((((.(((.......)))))))..))))).(((((...................)))))..... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_247 | Description | BnHNE2 |
| Aptamer chemistry | DNA | Length | 76 nt |
| GC content | 43.4% | Molecular weight | 23,658.00 Da |
| Molarity of 1 μg/μl solution | 42.27 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted binding |
| Sequence | GGCTTTACACTTTATGCTTCCGTACCAGAACATATCGAGTGATCCTGGGAGGATAACAATTTCACACAGGAAACAG | ||
| Applications | Binding to target proteins | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P08246 | Protein name | Neutrophil elastase (EC 3.4.21.37) (Bone marrow serine protease) (Elastase-2) (Human leukocyte elastase) (HLE) (Medullasin) (PMN elastase) |
| Gene name(s) | ELANE ELA2 | Organism | Neutrophil elastase (EC 3.4.21.37) (Bone marrow serine protease) (Elastase-2) (Human leukocyte elastase) (HLE) (Medullasin) (PMN elastase) |
| Status | reviewed in uniprot database | Length | 267 |
| Mass | 28,518 | Activity regulation | N/A |
| Involvement in disease | Cyclic haematopoiesis (CH) | Catalytic activity | Reaction=Hydrolysis of proteins, including elastin. Preferential cleavage: Val-|-Xaa > Ala-|-Xaa |
| PDB ID(s) | 1B0F;1H1B;1HNE;1PPF;1PPG;2RG3;2Z7F;3Q76;3Q77;4NZL;4WVP;5A09;5A0A;5A0B;5A0C;5A8X;5A8Y;5A8Z;5ABW;6E69;6F5M;6SMA;7CBK; |
 |
Function | Modifies the functions of natural killer cells, monocytes and granulocytes. |
Activity data
 |
Interaction ID | 252 |
| Target type | Protein | |
| Target unique ID | P08246 | |
| Activity | 49.2 nM | |
| Binding Conditions/Buffer | 20 mM HEPES pH 7.5, 150 mM NaCl, 6 mM KCl, 2 mM MgCl2, room temperature |
|
| Assay | Binding of BnHNE1, BnHNE2, BnHNE3, and their natural counterparts to HNE. Data are average ± SD of three independent determinations |
|
| PubMed ID | 31961667 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_84 | DNA | GGCTTTACACTTTATGCTTCCGTTGCAATTTTAATGGGAGGTACATGGCGGAATAACAATTTCACACAGGAAACAG | |
| Apta_246 | DNA | GGCTTTACACTTTATGCTTCCGGCCCGCGGAAATGATAACGCGTATGGTGGTATAACAATTTCACACAGGAAACAG | |
| Apta_248 | DNA | GGCTTTACACTTTATGCTTCCGGCCGAGGATTTTCATACCCCGCACGAACGCATAACAATTTCACACAGGAAACAG | |
| Apta_83 | DNA | GGCTTTACACTTTATGCTTCCGTAAAAACACAAACACACGCACATACATAAAATAACAATTTCACACAGGAAACAG | |
| Apta_82 | DNA | GGCTTTACACTTTATGCTTCCGAAACAACGCAGAAAGTCTTTACAACATTACATAACAATTTCACACAGGAAACAG | |
| Apta_807 | DNA | GGGTCTTCCTGGACTGTCGAAAATTCAGTATCGGGAGGTTACGTATTTGGTTTATAGATAGTAA | |
| Apta_1135 | DNA | GCCGAGATTGCACTTACTATCTCATCAATAAAGATTATAGGGTGAAGGTGATAGTAGGTGGTATATAGGGTAATTGAATAAGCTGGTATGCGC | |
| Apta_1107 | DNA | GCGCGATGGACTGTACCTTCGGCGTAACTGATATTCCTGATGATTCTGGCGAGACGTAGCACAAACCCGGTGTATA | |
| Apta_281 | DNA | CCGTAATACGACTCACTATAGGGGAGCTCGGTACCGAATTCATGTTGGTAGTTGGCTTGTGTTCGTGTGTTAAGCTTTCAGAGAGGATCCTT | |
| Apta_1108 | DNA | GTGCTTAGGTGCAGAACGCACACACGCTCGGTATGACGTGTTCGCTACGGTATAATTGCAGGCGCGAGCACAACAG | |
| Apta_1131 | DNA | GCCGAGATTGCACTTACTATCTCAAGAAGGGTAGTAAGAGGAGTGGGTGATTATGGGTAAGTTGTATGAGGTAATTGAATAAGCTGGTATGCGC | |
| Apta_1136 | DNA | GCCGAGATTGCACTTACTATCTAAAGAAAGGTAGTAAGAGTGGGTGATTATGGGTAAGTTGTATGAGGTAATTGAATAAGCTGGTATGCGC | |
| Apta_33 | DNA | GCGGTAAACTGCGTCCGAAGGGGCTGCAGTGACCCGAATGGGTCCGCCGCGAAGCG | |
| Apta_570 | DNA | GGCAAGATGTGCCCAGAACAGTTGCTTGTATGGTGGGGAGCGTCCATATTGGCTTAAAC | |
| Apta_992 | DNA | TCACTGACAGTAGGCCCATACGCACATTCAACAATCGGTAGTCCATACAACCGCGCCCCGTG | |
| Apta_1015 | DNA | ATACCAGCTTATTCAATTGACCCGTTTCGTTCCCTCTGGGAAGTTTAGCCCAGTTGCCTGGGCGATACCAAGATAGTAAGTGCAATCT | |
| Apta_1133 | DNA | GCCGAGATTGCACTTACTATCTAAAGAAGAGTAGTAAGAGGCGTGGGTGATTATGGGTAAGTTGTATGAGGTAATTGAATAAGCTGGTATGCGC | |
| Apta_823 | DNA | GGGATACCAGCTTATTCAATTTGATTCCATCTTCCTGGACTGTCGAAAATTCAGTATCGGGAGGTTACGTATTTGGTTTATAGATAGTAAGTGCAATCT | |
| Apta_934 | DNA | GACGCTTACTCAGGTGTGACTCGTGAACCCACCTCAGGGCATCTTACATTTCTTCTAAGTTGTTACCATGTTTCGAAGGACGCAGATGAAGTCTC | |
| Apta_1128 | DNA | GCCGAGATTGCACTTACTATCTCAATAGGGTAAAAAAAAAAAGTTGGTCCTATGGGTGGGTTGTTCAGTAATTGAATAAGCTGGTATGCGC |
DEV TOOL
 |
 |
 |
 |