AptaDB: Result

Interaction ID: 1052

Aptamer sequence: TAACCACCCCACCTCGCTCCCGTGACACTAATGCTAATTCCAA

Target unique ID: P08962

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_969	CD63-2	Protein	CD63_HUMAN	78.43 nM	pH 7.4 PBS containing 2.0 mM MgCl2, 0.02% Tween 20, and 100 µg/mL Salmon Sperm DNA	33261145

Aptamer Sequence:	TAACCACCCCACCTCGCTCCCGTGACACTAATGCTAATTCCAA
The optimal secondary structure in dot-bracket notation:	.............((((....))))..................
The centroid secondary structure in dot-bracket notation:	.............((((....))))..................

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_969	Description	CD63-2
Aptamer chemistry	DNA	Length	43 nt
GC content	51.2%	Molecular weight	12,956.35 Da
Molarity of 1 μg/μl solution	77.18 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted therapy
Sequence	TAACCACCCCACCTCGCTCCCGTGACACTAATGCTAATTCCAA
Applications	Binding to target proteins

Type	Detail	Type	Detail
Uniprot ID	P08962	Protein name	CD63 antigen (Granulophysin) (Lysosomal-associated membrane protein 3) (LAMP-3) (Lysosome integral membrane protein 1) (Limp1) (Melanoma-associated antigen ME491) (OMA81H) (Ocular melanoma-associated antigen) (Tetraspanin-30) (Tspan-30) (CD antigen CD63)
Gene name(s)	CD63 MLA1 TSPAN30	Organism	CD63 antigen (Granulophysin) (Lysosomal-associated membrane protein 3) (LAMP-3) (Lysosome integral membrane protein 1) (Limp1) (Melanoma-associated antigen ME491) (OMA81H) (Ocular melanoma-associated antigen) (Tetraspanin-30) (Tspan-30) (CD antigen CD63)
Status	reviewed in uniprot database	Length	238
Mass	25,637	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	N/A
Function	Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades.

	Interaction ID	1052
	Target type	Protein
	Target unique ID	P08962
	Activity	78.43 nM
	Binding Conditions/Buffer	pH 7.4 PBS containing 2.0 mM MgCl2, 0.02% Tween 20, and 100 µg/mL Salmon Sperm DNA
	Assay	Analysing equilibrium dissociation constants (Kd) of CD63-2 aptamer to CD63 molecule by ELISA
	PubMed ID	33261145

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_238	DNA	CACACATTCCCGTCCTCGATACGTCTAGGCTTAGTGCCACTTGCTTAATC	54.00%
Apta_448	DNA	TAACTCACTCCATACTCACTTGCTGATTCGCCAACAACACACCCTTAAACAGTCCC	53.57%
Apta_220	DNA	ATCAACTCACCGTAGGTCTAGTGAGAACTTCAAGTCTACT	53.49%
Apta_436	DNA	CCCCCGCATCACGCCGTGGTGCGATTGACACAATTGCAAT	53.49%
Apta_439	DNA	TAACACTCGATTCTCCTAGCCCGCTAGAAATTCCCCTCCC	53.49%
Apta_446	DNA	AGCCATGACGATGTCGTTACGTAGATGCATAGACTCCTAA	53.49%
Apta_472	DNA	CCCCCACATTCAAATGGCCCCCCTACTCAATTACTGCACA	53.49%
Apta_1191	DNA	TAATACTACCAACTTCTTTGCCTGGCGTAAGTAACAGTCA	53.49%
Apta_1228	DNA	GGCCACCAAACCACACCGTCGCAACCGCGAGAACCGAAGTAGTGATCATGTCCCTGCGTG	53.33%
Apta_1003	DNA	ATAGGAGTCGACCGACCAGACCCCGCCTCTCCTTTCATGTGACACAATGGTGCGTCTACATCTAGACTC	52.17%
Apta_1004	DNA	ATAGGAGTCGACCGACCAGACCCGACCTCTCTTTCACTGTGACACAATGGTGCGTCTACATCTAGACTC	52.17%
Apta_1010	DNA	ATAGGAGTCGACCGACCAGACCGACCCTCGTCGTACCAACTGATGACCTAGTGGCGTCTACATCTAGACTC	52.11%
Apta_1072	DNA	TTGAAGTGACTCCCACCCCGCCCCACCGTCCTTGCCACCGTTCCGCCATAGCAGGT	51.79%
Apta_1007	DNA	ATAGGAGTCGACCGACCAGACCCGACCTCTCTTTCATGTGACACAATGGTGCGTCTACATCTAGACTC	51.47%
Apta_997	DNA	ATAGGAGTCGACCGACCAGACCCCGCCTCTCCTTTTCATGTGACACAATGGTGCGTCTACATCTAGACTC	51.43%
Apta_998	DNA	ATAGGAGTCGACCGACCAGACCAACCCTACGCGTACCAACCAGATGACCTACGTGCGTCTACATCTAGACTC	51.39%
Apta_7	DNA	CCAGTCTCCCGTTTACCGCGCCTACACATGTCTGAATGCC	51.16%
Apta_165	DNA	TGGACGAACTGCCCTCAGCTACTTTCATGTTGCTGACGCA	51.16%
Apta_183	DNA	TAGCACTGTGCGGCGCGTATTGTTCTAATCTGATTACATT	51.16%
Apta_438	DNA	AGCCATGACGATGTCGTTACGTAGATGCAGAGACTCCTAA	51.16%

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