Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1027
Aptamer sequence: CGGGTGCCTTAAAGTGTAGGTGGGAGGGGGATGATTATGTACGATCAAGT
Target unique ID: Other-38
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_941 | QA-92 | Other | Soft-shelled turtle iridovirus (STIV) | 71.2 nM | 100 mM NaCl, 2.5 mM MgCl2, 20 mM Tris-HCl (pH 7.5) | 26419355 |
Structure information of aptamer
| Aptamer Sequence: | CGGGTGCCTTAAAGTGTAGGTGGGAGGGGGATGATTATGTACGATCAAGT |
| The optimal secondary structure in dot-bracket notation: | .....((((........))))..........(((((......)))))... |
| The centroid secondary structure in dot-bracket notation: | .....((((........))))..........(((((......)))))... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_941 | Description | QA-92 |
| Aptamer chemistry | DNA | Length | 50 nt |
| GC content | 50.0% | Molecular weight | 15,681.06 Da |
| Molarity of 1 μg/μl solution | 63.77 μM | Number of G-quadruplexes | 2 |
| G-Score | 20 | Function | Targeted therapy |
| Sequence | CGGGTGCCTTAAAGTGTAGGTGGGAGGGGGATGATTATGTACGATCAAGT | ||
| Applications | Inhibited STIV infection in vitro and in vivo, with aptamer QA-36 demonstrating the greatest protective effect against STIV and inhibiting STIV infection in a dose-dependent manne | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Target ID | Other-38 | Name | Soft-shelled turtle iridovirus (STIV) |
Activity data
 |
Interaction ID | 1027 |
| Target type | Other | |
| Target unique ID | Other-38 | |
| Activity | 71.2 nM | |
| Binding Conditions/Buffer | 100 mM NaCl, 2.5 mM MgCl2, 20 mM Tris-HCl (pH 7.5) |
|
| Assay | Affinity of STIV-selected ssDNA aptamer interactions by ELISA. STIV was incubated with increasing concentrations of 5′-biotinylated aptamer. After addition of streptavidin-HRP, the amount of STIV-aptamer complex was calculated and graphed as a function of aptamer concentration. The graph was fit to the equation Y = Bmax*X/(Kd + X) using SigmaPlot software. Results for each aptamer was presented as the mean ± SD of three independent experiments |
|
| PubMed ID | 26419355 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_691 | DNA | CGACGCGCGTTGGTGGTGGATGGTGTGTTACACGTGTTGT | |
| Apta_747 | DNA | TGGGGTTATTAATTTTGGGTGGGGGGGAAGATGTAGCATCCGACG | |
| Apta_782 | DNA | AGGTGTGACTCGATCTGTGGGGGTTGGGGGGTGGTTTTTCGGAA | |
| Apta_988 | DNA | AAGGATGACTGACGACGATGGGAATGGAGAGGAAGGGGATATAGGTATGTTGGATGTAG | |
| Apta_745 | DNA | GGTTGCATGCCGTGGGGAGGGGGGTGGGTTTTATAGCGTACTCAG | |
| Apta_862 | DNA | TGTGCCAAAGAGAGTGGTGGGGGGGTGGGCGGAACTCGCG | |
| Apta_464 | DNA | GTCAGATTCCACTATAGTAGGTTGGGTAGGGTGGTCGCAGTGGATGATATGTCGTAGGGG | |
| Apta_46 | DNA | CGGGTGGGAGTTGTGGGGGGGGGTGGGAGGGTT | |
| Apta_579 | DNA | ACTGAGGGCACGGACAGGAGGGGGAGAGATGGCGTGAGGT | |
| Apta_590 | DNA | ACCATCTGTGTAAGGGGTAAGGGGTGGGGGTGGGTACGTCT | |
| Apta_1062 | DNA | GGGGTCTTGCAGGTCCCGTAGGAGGGGCCATTGGAGTGGGG | |
| Apta_756 | DNA | GGGGGACACGGAGGTGGTGGAAAGGCTAAGATTTGATGATGAGTAGTGTGGT | |
| Apta_76 | DNA | CAGCGCTAGGGCTTTTAGCGTAATGGGTAGGGTGGTGCGGTGCAGATATCGGAATTGGTG | |
| Apta_1071 | DNA | TTGAAGTGACTCCGCACTGGGTGGGTGGGAGGGTCGTGCGGCTGGTCATAGCAGGGT | |
| Apta_1011 | DNA | GCTGTGTGACTCCTGCAATGGTAGCGGGTAGGGGAGGGAGGGTGAATGGAGGATGTTCATGGCAGCTGTATCTTGTCTCC | |
| Apta_618 | DNA | GCGGCAAATCAGCATACGAGTGTACAGGGACGGGACGGGTGGGTAAAAGGTGTCGCCTCAG | |
| Apta_667 | DNA | GCGGCGGTACCAGGTCAAAGGTGGGTGAGGGGACGCCAAGAGTCCCCGCGGTTACATAGAG | |
| Apta_77 | DNA | GGTGTGGGCGACATGGGGGTTGGTGGTGTAGGAGCGCCAT | |
| Apta_266 | DNA | TGGATGGTAGCTCGGTCGGGGTGGGTGGGTTGGCAAGTCT | |
| Apta_478 | DNA | CTTCACTTTCGTGGACGGTGGCGTCGGGGCGGTTTGTATG |
DEV TOOL
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