ProForma 2.0
validated simple linear
IKKIVSKIKKLLNH
Back to Primary literature
Primary-literature peptidehigh evidence PepDiana evidence grade for a literature-derived identity; Medium indicates retained review flags, while High has no unresolved review flag.
PEPDIA-PLP-001298
L-K5
Aliases: L-K5
Normalized sequence
IKKIVSKIKKLLNH
14 amino acids
Release PepDiana-V1-20260620
34
Activity records
1
Source papers
32
Positive
2
Negative / threshold
Versioned scientific descriptors
Physicochemical properties and standard representations
Reported properties
No separate physicochemical property was reported in the primary-literature identity record. PepDiana release values are shown as computed properties.
Computed properties
physchem-20260620Molecular weight
1662.115 Da
Molecular formula
C78H144N22O17
Theoretical pI
10.6025
Net charge at pH 7
4.8423
GRAVY
-0.1214
Instability index
-27.1
Aromaticity
0
Topological polar surface area
663.72 Ų
cLogP
-2.9343
Hydrogen-bond donors
23
Hydrogen-bond acceptors
23
Rotatable bonds
63
Method: PepDiana sequence/structure descriptor pipeline
Tool: Biopython+RDKit · Biopython 1.85; RDKit 2024.09.6
Calculated: 2026-06-20T11:01:38.358971+00:00
Assumptions: ph=7; termini=free N-terminus and free C-terminus for simple linear peptides; stereochemistry=standard L-amino acids; smiles_length_limit=200
Sequence representations
HELM 2.0
validated simple linear
PEPTIDE1{I.K.K.I.V.S.K.I.K.K.L.L.N.H}$$$$V2.0
Canonical SMILES
validated
CCC(C)C(N)C(=O)NC(CCCCN)C(=O)NC(CCCCN)C(=O)NC(C(=O)NC(C(=O)NC(CO)C(=O)NC(CCCCN)C(=O)NC(C(=O)NC(CCCCN)C(=O)NC(CCCCN)C(=O)NC(CC(C)C)C(=O)NC(CC(C)C)C(=O)NC(CC(N)=O)C(=O)NC(Cc1c[nH]cn1)C(=O)O)C(C)CC)C(C)C)C(C)CC
Isomeric SMILES
validated
CC[C@H](C)[C@H](N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)O)[C@@H](C)CC)C(C)C)[C@@H](C)CC
Paper-level observations
Experimental evidence grouped by activity
Anticancer 16 records
PMC12429139
16 observationsAnticancerAntibacterialPositive observation
Record detail The tumor volume decreased 21.44% ... after treatment with L-K5 at doses of 2.5 ... mg/kg (Section 2.1).
- Measure
- percent_inhibition
- Value
- 21.44%
- Target
- Mus musculus
- Assay
- Not reported by source
AnticancerAntibacterialPositive observation
Record detail The tumor volume decreased ... 23.44% ... after treatment with L-K5 at doses of ... 5 ... mg/kg (Section 2.1).
- Measure
- percent_inhibition
- Value
- 23.44%
- Target
- Mus musculus
- Assay
- Not reported by source
AnticancerAntibacterialPositive observation
Record detail The tumor volume decreased ... 32.6% after treatment with L-K5 at doses of ... 10 mg/kg (Section 2.1).
- Measure
- percent_inhibition
- Value
- 32.6%
- Target
- Mus musculus
- Assay
- Not reported by source
AnticancerAnti-metastaticPositive observation
Record detail The inhibition rates were 18.69% ... in the L-K5 groups at 2.5 ... mg/kg (Section 2.1).
- Measure
- percent_inhibition
- Value
- 18.69%
- Target
- Mus musculus
- Assay
- Not reported by source
AnticancerAnti-metastaticPositive observation
Record detail The inhibition rates were ... 26.17% ... in the L-K5 groups at ... 5 ... mg/kg (Section 2.1).
- Measure
- percent_inhibition
- Value
- 26.17%
- Target
- Mus musculus
- Assay
- Not reported by source
AnticancerAnti-metastaticPositive observation
Record detail The inhibition rates were ... 46.73% ... in the L-K5 groups at ... 10 mg/kg (Section 2.1).
- Measure
- percent_inhibition
- Value
- 46.73%
- Target
- Mus musculus
- Assay
- Not reported by source
AnticancerAnti-metastaticPositive observation
Record detail The polarized M2 macrophages treated with 10 ... μM L-K5 reduced MCF-7 cell migration by 80.8% (Section 2.4).
- Measure
- percent_inhibition
- Value
- 80.8%
- Target
- Homo sapiens
- Assay
- Not reported by source
AnticancerAnti-metastaticPositive observation
Record detail The polarized M2 macrophages treated with ... 20 μM L-K5 reduced MCF-7 cell migration by ... 95.1% (Section 2.4).
- Measure
- percent_inhibition
- Value
- 95.1%
- Target
- Homo sapiens
- Assay
- Not reported by source
AnticancerAnti-metastaticPositive observation
Record detail L-K5 exhibited the best inhibition, with decreases of 54.7% ... when the L-K5 concentration was 10 ... μM (Section 2.4).
- Measure
- percent_inhibition
- Value
- 54.7%
- Target
- Homo sapiens
- Assay
- Not reported by source
AnticancerAnti-metastaticPositive observation
Record detail L-K5 exhibited the best inhibition, with decreases of ... 73% when the L-K5 concentration was ... 20 μM (Section 2.4).
- Measure
- percent_inhibition
- Value
- 73%
- Target
- Homo sapiens
- Assay
- Not reported by source
AnticancerAnti-metastaticPositive observation
Record detail Treatment with L-K5 ... at a concentration of 20 μM downregulated pERK expression by 91.2% (Section 2.4).
- Measure
- percent_inhibition
- Value
- 91.2%
- Target
- Homo sapiens
- Assay
- Not reported by source
AnticancerAnti-metastaticPositive observation
Record detail Treatment with 20 μM L-K5 ... reduced snail mRNA expression by 69.1% (Section 2.4).
- Measure
- percent_inhibition
- Value
- 69.1%
- Target
- Homo sapiens
- Assay
- Not reported by source
AnticancerAnti-metastaticPositive observation
Record detail L-K5 (20 μM) had the greatest inhibitory effect (74.4%) on twist mRNA (Section 2.4).
- Measure
- percent_inhibition
- Value
- 74.4%
- Target
- Homo sapiens
- Assay
- Not reported by source
AnticancerAnti-metastaticPositive observation
Record detail The protein expression was downregulated by approximately 65% after treatment with the three peptides compared to the IL-4-treated group (Section 2.4).
- Measure
- percent_inhibition
- Value
- ~65%
- Target
- Homo sapiens
- Assay
- Not reported by source
AnticancerAnti-metastaticPositive observation
Record detail L-K5 (10 μM ...) increased the protein expression of E-cadherin by 66.3% (Section 2.4).
- Measure
- percent_inhibition
- Value
- 66.3% increase
- Target
- Homo sapiens
- Assay
- Not reported by source
AnticancerAnti-metastaticPositive observation
Record detail L-K5 (... 20 μM) increased the protein expression of E-cadherin by ... 69.7% (Section 2.4).
- Measure
- percent_inhibition
- Value
- 69.7% increase
- Target
- Homo sapiens
- Assay
- Not reported by source
Immunomodulatory 16 records
PMC12429139
16 observationsImmunomodulatoryAnti-inflammatoryPositive observation
Record detail L-K5 at doses of 2.5 ... mg/kg decreased the release of IL-10 by 14.14% (Section 2.1).
- Measure
- percent_inhibition
- Value
- 14.14%
- Target
- Mus musculus
- Assay
- Not reported by source
ImmunomodulatoryAnti-inflammatoryPositive observation
Record detail L-K5 at doses of ... 5 ... mg/kg decreased the release of IL-10 by ... 24.42% (Section 2.1).
- Measure
- percent_inhibition
- Value
- 24.42%
- Target
- Mus musculus
- Assay
- Not reported by source
ImmunomodulatoryAnti-inflammatoryPositive observation
Record detail L-K5 at doses of ... 10 mg/kg decreased the release of IL-10 by ... 29.2% (Section 2.1).
- Measure
- percent_inhibition
- Value
- 29.2%
- Target
- Mus musculus
- Assay
- Not reported by source
ImmunomodulatoryAnti-inflammatoryPositive observation
Record detail L-K5 at doses of 2.5 ... mg/kg decreased ... Arg-1 by 46.62% (Section 2.1).
- Measure
- percent_inhibition
- Value
- 46.62%
- Target
- Mus musculus
- Assay
- Not reported by source
ImmunomodulatoryAnti-inflammatoryPositive observation
Record detail L-K5 at doses of ... 5 ... mg/kg decreased ... Arg-1 by ... 60.36% (Section 2.1).
- Measure
- percent_inhibition
- Value
- 60.36%
- Target
- Mus musculus
- Assay
- Not reported by source
ImmunomodulatoryAnti-inflammatoryPositive observation
Record detail L-K5 at doses of ... 10 mg/kg decreased ... Arg-1 by ... 63.7% (Section 2.1).
- Measure
- percent_inhibition
- Value
- 63.7%
- Target
- Mus musculus
- Assay
- Not reported by source
ImmunomodulatoryAnti-inflammatoryPositive observation
Record detail 10 μM ... L-K5 reduced the IL-10 content by 59.6% (Section 2.2).
- Measure
- percent_inhibition
- Value
- 59.6%
- Target
- Homo sapiens
- Assay
- Not reported by source
ImmunomodulatoryAnti-inflammatoryPositive observation
Record detail 20 μM L-K5 reduced the IL-10 content by ... 62.3% (Section 2.2).
- Measure
- percent_inhibition
- Value
- 62.3%
- Target
- Homo sapiens
- Assay
- Not reported by source
ImmunomodulatoryAnti-inflammatoryPositive observation
Record detail 10 μM ... L-K5 reduced ... Arg-1 by 83.0% (Section 2.2).
- Measure
- percent_inhibition
- Value
- 83.0%
- Target
- Homo sapiens
- Assay
- Not reported by source
ImmunomodulatoryAnti-inflammatoryPositive observation
Record detail 20 μM L-K5 reduced ... Arg-1 by ... 84.9% (Section 2.2).
- Measure
- percent_inhibition
- Value
- 84.9%
- Target
- Homo sapiens
- Assay
- Not reported by source
ImmunomodulatoryAnti-inflammatoryPositive observation
Record detail L-K5 exhibited the most significant inhibitory effect, with inhibition rates of 63.3% ... at peptide concentrations of 10 ... μM (Section 2.3).
- Measure
- percent_inhibition
- Value
- 63.3%
- Target
- Homo sapiens
- Assay
- Not reported by source
ImmunomodulatoryAnti-inflammatoryPositive observation
Record detail L-K5 exhibited the most significant inhibitory effect, with inhibition rates of ... 70.6% at peptide concentrations of ... 20 μM (Section 2.3).
- Measure
- percent_inhibition
- Value
- 70.6%
- Target
- Homo sapiens
- Assay
- Not reported by source
ImmunomodulatoryAnti-inflammatoryPositive observation
Record detail KLF4 expression was downregulated by approximately 80% in IL4-induced U937 macrophages treated with 10 and 20 μM of ... L-K5 (Section 2.3).
- Measure
- percent_inhibition
- Value
- ~80%
- Target
- Homo sapiens
- Assay
- Not reported by source
ImmunomodulatoryAnti-inflammatoryPositive observation
Record detail L-K5 at peptide concentrations of 10 and 20 μM reduced the phosphorylation of STAT6 by approximately 76% (Section 2.3).
- Measure
- percent_inhibition
- Value
- ~76%
- Target
- Homo sapiens
- Assay
- Not reported by source
ImmunomodulatoryAnti-inflammatoryPositive observation
Record detail L-K5 ... at a concentration of 20 μM reduced the TGF-β1 levels by ... 76.3% (Section 2.4).
- Measure
- percent_inhibition
- Value
- 76.3%
- Target
- Homo sapiens
- Assay
- Not reported by source
ImmunomodulatoryAnti-inflammatoryPositive observation
Record detail L-K5 ... at a concentration of 20 μM reduced the EGF level by ... 88% (Section 2.4).
- Measure
- percent_inhibition
- Value
- 88%
- Target
- Homo sapiens
- Assay
- Not reported by source
Toxicity 2 records
PMC12429139
2 observationsToxicityCytotoxicNegative / threshold
Record detail cell viability was greater than 80% when the concentrations of ... L-K5 ... were 10 and 20 μM (Section 2.2, Figure S1A).
- Measure
- cell_viability
- Value
- >80%
- Target
- Homo sapiens
- Assay
- Not reported by source
ToxicityCytotoxicNegative / threshold
Record detail the viability of MCF-7 cells was greater than 80% after treatment with 10 and 20 μM of ... L-K5 (Section 2.4, Figure S1B).
- Measure
- cell_viability
- Value
- >80%
- Target
- Homo sapiens
- Assay
- Not reported by source
Citation and release provenance
Cite this identity as PepDiana record PEPDIA-PLP-001298, release PepDiana-V1-20260620. Experimental observations retain their paper, mention, and activity-record identifiers.
Record updated: 2026-06-22T07:14:26.229247+00:00