Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 465
Aptamer sequence: GGAUGGCAGCGGAGAGUUGCUUGGAAUGCGUUAUAGUCUCUUAGGUGUGUACAGCCCACACCACUCUCC
Target unique ID: Q3SY56
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_460 | S05 | Protein | SP6_HUMAN | 9.5 nM | 50 mM Tris-HCl, pH 7.6, 1 M NaCl, 10 mM 2-mercaptoethanol, 10% glycerol at 4 °C overnight at -20 °C until use | 22426482 |
Structure information of aptamer
| Aptamer Sequence: | GGAUGGCAGCGGAGAGUUGCUUGGAAUGCGUUAUAGUCUCUUAGGUGUGUACAGCCCACACCACUCUCC |
| The optimal secondary structure in dot-bracket notation: | ..........(((((((.((.......))..............((((((.......))))))))))))) |
| The centroid secondary structure in dot-bracket notation: | ..........(((((((.((.......))..............((((((.......))))))))))))) |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_460 | Description | S05 |
| Aptamer chemistry | RNA | Length | 69 nt |
| GC content | 53.6% | Molecular weight | 22,150.10 Da |
| Molarity of 1 μg/μl solution | 45.15 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted therapy |
| Sequence | GGAUGGCAGCGGAGAGUUGCUUGGAAUGCGUUAUAGUCUCUUAGGUGUGUACAGCCCACACCACUCUCC | ||
| Applications | Inhibits transcription of SP6 RNA polymerase | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | Q3SY56 | Protein name | Transcription factor Sp6 (Krueppel-like factor 14) |
| Gene name(s) | SP6 KLF14 | Organism | Transcription factor Sp6 (Krueppel-like factor 14) |
| Status | reviewed in uniprot database | Length | 376 |
| Mass | 39,840 | Activity regulation | N/A |
| Involvement in disease | N/A | Catalytic activity | N/A |
| PDB ID(s) | N/A |
 |
Function | Promotes cell proliferation. |
Activity data
 |
Interaction ID | 465 |
| Target type | Protein | |
| Target unique ID | Q3SY56 | |
| Activity | 9.5 nM | |
| Binding Conditions/Buffer | 50 mM Tris-HCl, pH 7.6, 1 M NaCl, 10 mM 2-mercaptoethanol, 10% glycerol at 4 °C overnight at -20 °C until use |
|
| Assay | Estimation of dissociation constant (KD) by filter-binding assay |
|
| PubMed ID | 22426482 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_1223 | RNA | GGAGGCGCCAACUGAAUGAAAGGUUACGCUUCAACGCCGUGCAGCUGCUUCGGCAGGCUUGUGAGUACAUGGCCCAUUGAUCCGUAACUAGUCGCGUCAC | |
| Apta_816 | RNA | GGGUUCACUGCAGACUUGACGAAGCUUGAGAGAUGCCCCCUGAUGUGCAUUCUUGUUGUGUUGCGGCAAUGGAUCCACAUCUACGAAUUC | |
| Apta_132 | RNA | GGGAGGACGAUGCGGCGUUUCCUCUGGUUCGUCCCCAGACGACUCGCCCGA | |
| Apta_856 | RNA | UGGGCCGGAGGUUAGCUUGCCCAUGGCAAGCAGGGCGCCACGGACCCA | |
| Apta_984 | RNA | GGGAGGACGAUGCGGCGUAUUGCGCGAGGAUUAUCCGCUCAUCGUUGUUGUUGUGCAGACGACUCGCCCGA | |
| Apta_1224 | RNA | GGAGGCGCCAACUGAAUGAAUGUUCUGGAUCUGCUAGAGACUAUCUGCUUCGGCAGCACUCUCCGUUAACAACACGUACAUCCGUAACUAGUCGCGUCAC | |
| Apta_414 | RNA | GGGAGGACGAUGCGGCCGAUUAGAACUGGGCUGAGGCGUUCUGCAUUUCGGUGAUCAGACGACUCGCUGAGGAUCCGAGA | |
| Apta_562 | RNA | GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGCCUUUAGGGCCAAGUGUGGUGAAAGACACACUCGAAACGGUAGCGAGAGCUC | |
| Apta_6 | RNA | GGAAAGACGCUAGCGAAUUGGUUCCUCGAAAGGGGAAAGCGUUAUUAAGAAACCAAAAUUUCC | |
| Apta_129 | RNA | GGGAGGACGAUGCGGACACCGUUAAUCUGAGGCCCUGUCCUAUUCCUUCACGCCUCAGA | |
| Apta_315 | RNA | GGGAGGACGAUGCGGUCGUAUUAUCCGCUGCACGCCAGACGACUCGCCCGA | |
| Apta_576 | RNA | GGCACCAAAGCUGAAGUAGCGGGAUAACUCAAAUUACUUUAGGUGUAUGAAGGUGAAACUAGCAAUGAA | |
| Apta_668 | RNA | GGAACCCAACUAGGCGUUUGAGGGGAUUCGGCCACGGUAACAACCCCUC | |
| Apta_1222 | RNA | GGAGGCGCCAACUGAAUGAAACCGUGCUAAGGAGUUGGACUCCGCUGCUUCGGCAGAGCGAUAUAGUGCCAGCUGAUACAUCCGUAACUAGUCGCGUCAC | |
| Apta_1226 | RNA | GGAGGCGCCAACUGAAUGAAGGUGUAUUUCAGUUAAAGAACUUGCUGCUUCGGCAGCGUUCUGAGUUACUAGUCCAAUAAUCCGUAACUAGUCGCGUCAC | |
| Apta_410 | RNA | GGGAGAGCGGAAGCGUGCUGGGCCUGUCGUUCGCUGUGUCUUGCUACGUUACGUUACACGGUUGGCAUAACCCAGAGGUCGAUGG | |
| Apta_300 | RNA | AUACCAGCUUAUUCAAUUGCCUGAAACUGCUGCAUCGUCUCCCACGUAUUACACAUGAAGAUAGUAAGUGCAAUCU | |
| Apta_624 | RNA | GGGAAUGGAUCCACAUCUACGAAUUCGAAUUGCCGUAAUUUCCCGUGGAGCGAUGCUUCACUGCAGACUUGACGAAGCUU | |
| Apta_43 | RNA | GGGAGAGCGGAAGCGUGCUGGGCCACCAUCCGUAACUAGCUAAUACUUGUUAUCUUUUUAUUUUCAUAACCCAGAGGUCGAUGGAUCCCCCC | |
| Apta_130 | RNA | GGGAGGACGAUGCGGUGGUAGCGCGAUAUAGCGCUGGUAGGGUUGCCGGUGAUCAGACGACUCGCCCGA |
DEV TOOL
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