AptaDB: Result

Interaction ID: 836

Aptamer sequence: GGGCGGGGAGTAGGGAGAGGGGTTTCCATCGGCGACAGAGGAGTTATGTGTGT

Target unique ID: CCL-2

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_754	aptamer 14	Cell	Hela cells	7.1 nM	1% BSA in PBS with salts	22536456

Aptamer Sequence:	GGGCGGGGAGTAGGGAGAGGGGTTTCCATCGGCGACAGAGGAGTTATGTGTGT
The optimal secondary structure in dot-bracket notation:	..((.((......(((((....))))).)).))....................
The centroid secondary structure in dot-bracket notation:	.............(((((....)))))..........................

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_754	Description	aptamer 14
Aptamer chemistry	DNA	Length	53 nt
GC content	60.4%	Molecular weight	16,710.67 Da
Molarity of 1 μg/μl solution	59.84 μM	Number of G-quadruplexes	3
G-Score	62	Function	Aptasensor
Sequence	GGGCGGGGAGTAGGGAGAGGGGTTTCCATCGGCGACAGAGGAGTTATGTGTGT
Applications	Molecular probes of HPV-associated cervical cancer cells

Type	Detail	Type	Detail
ATCC ID	CCL-2	Cell name	Hela cells
Category	Human cells	Organism	Homo sapiens, human
Product type	N/A	Cell type	epithelial cell
Morphology	epithelial	Tissue	Uterus; Cervix
Disease	Adenocarcinoma	Product format	Frozen
Antigen expression	N/A	Genes expressed	Lysophosphatidylcholine (lyso-PC) induces AP-1 activity and c-jun N-terminal kinase activity (JNK1) by a protein kinase C-independent pathway; keratin positive by immunoperoxidase staining
Cell description	HeLa cells are the first immortal human cells to be grown in culture and the basis for countless significant scientific discoveries. They were isolated in 1951 from a cervical carcinoma derived from a 31-year-old patient.
Application(s)	3D cell culture

	Interaction ID	836
	Target type	Cell
	Target unique ID	CCL-2
	Activity	7.1 nM
	Binding Conditions/Buffer	1% BSA in PBS with salts
	Assay	Binding equilibrium for aptamers and their HF cell epitopes
	PubMed ID	22536456

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_487	DNA	CGGGGAGTTGGGAGGGGGGGCAAAGCCTGTGTATTTATCTTCTGT	60.38%
Apta_565	DNA	GCGGGGTTGGGCGGGTGGGTTCGCTGGGCAGGGGGCGAGTG	58.49%
Apta_749	DNA	GATCGGTGGGTGGGGGGGTTGGAGATCATCCTCAGGGATTACGTC	56.60%
Apta_830	DNA	GACGGTGGCAGGGAAAGGGGTCGGGCATATGGCGGAGGGG	56.60%
Apta_940	DNA	TGTGCGGGGGAGGGGAGTGGCGCTGTTGGTGCGGGTATAGCGCGTGGTGT	56.60%
Apta_1259	DNA	GGCGCGGCATGGTGTGGACTCCAGGCGGTAGGGATGTCGT	56.60%
Apta_449	DNA	CGCACTATGTTTTACGAGCCGTTTCCTCGGCAGATAGTAAGTGCG	54.72%
Apta_1189	DNA	GCGGGCGGGGGGAGGGCGGCCGTGGGCTGCGAGTGGGAGG	54.72%
Apta_508	DNA	GCGGGCGATCGGCGGGGGGTGCGTGCGCTCTGTGCCAGGGGGTGGGACAGATCATATGGGGGTGCT	54.55%
Apta_617	DNA	CAATGGGGTCGGGCGGGCCGGGTGTCATGGTGGACGGAGATGGGACGTAGAGGGCGGT	53.45%
Apta_608	DNA	TGAGGCGGGTGGGTGGGTTGAATACGCTGATCACCCCATCGGAGAACGTTAAGGCGCTTT	53.33%
Apta_732	DNA	AAGGAGCAGCGTGGAGGATAGCTGTCATCGGCTTATGTGCTTTCCAGGGTCCGTGCTGTCCGTTGTTAGGGTGTGTCGTCGTGGT	52.94%
Apta_143	DNA	GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCGATAGAGGTAGTTTCG	52.83%
Apta_264	DNA	GGCGTTGTCGGGCGCAGGTGTAGGCCTCGTGGTGGTGGGT	52.83%
Apta_488	DNA	CGACCAGGGTGGGAGGGGGACGGGCTGGCGGCGCGCGGTAATG	52.83%
Apta_492	DNA	GCGAGGTGTTCGAGAGTTAGGGGCGACATGACCAAACGTT	52.83%
Apta_556	DNA	GGCTCTCGGGACGACATGGATTTTCCATCAACGAAGTGCGTCCGTCCC	52.83%
Apta_693	DNA	CGAGTTGAGCCGGGCGCGGTACGGGTACTGGTATGTGTGG	52.83%
Apta_756	DNA	GGGGGACACGGAGGTGGTGGAAAGGCTAAGATTTGATGATGAGTAGTGTGGT	52.83%
Apta_879	DNA	GGGGGGGTGAGACATCGGGCATGTTCATGGGTGGGGGGTA	52.83%

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