AptaDB: Result

Interaction ID: 837

Aptamer sequence: GGGGAGGGAGACACAGTCATGGAGCAGTTATTAGGGTGTACCGGGTGTAGT

Target unique ID: CCL-2

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_755	aptamer 20	Cell	Hela cells	1.6 nM	1% BSA in PBS with salts	22536456

Aptamer Sequence:	GGGGAGGGAGACACAGTCATGGAGCAGTTATTAGGGTGTACCGGGTGTAGT
The optimal secondary structure in dot-bracket notation:	..........(((((.((..((.(((.((....)).))).)))).))).))
The centroid secondary structure in dot-bracket notation:	............(((.....((.(((.((....)).))).))...)))...

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_755	Description	aptamer 20
Aptamer chemistry	DNA	Length	51 nt
GC content	54.9%	Molecular weight	16,020.26 Da
Molarity of 1 μg/μl solution	62.42 μM	Number of G-quadruplexes	3
G-Score	48	Function	Aptasensor
Sequence	GGGGAGGGAGACACAGTCATGGAGCAGTTATTAGGGTGTACCGGGTGTAGT
Applications	Molecular probes of HPV-associated cervical cancer cells

Type	Detail	Type	Detail
ATCC ID	CCL-2	Cell name	Hela cells
Category	Human cells	Organism	Homo sapiens, human
Product type	N/A	Cell type	epithelial cell
Morphology	epithelial	Tissue	Uterus; Cervix
Disease	Adenocarcinoma	Product format	Frozen
Antigen expression	N/A	Genes expressed	Lysophosphatidylcholine (lyso-PC) induces AP-1 activity and c-jun N-terminal kinase activity (JNK1) by a protein kinase C-independent pathway; keratin positive by immunoperoxidase staining
Cell description	HeLa cells are the first immortal human cells to be grown in culture and the basis for countless significant scientific discoveries. They were isolated in 1951 from a cervical carcinoma derived from a 31-year-old patient.
Application(s)	3D cell culture

	Interaction ID	837
	Target type	Cell
	Target unique ID	CCL-2
	Activity	1.6 nM
	Binding Conditions/Buffer	1% BSA in PBS with salts
	Assay	Binding equilibrium for aptamers and their HF cell epitopes
	PubMed ID	22536456

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_879	DNA	GGGGGGGTGAGACATCGGGCATGTTCATGGGTGGGGGGTA	64.71%
Apta_1230	DNA	GGCGATAGGCAGTGTTGCGGGGTCGGAGAGCGAGGTAATAGCGTGTATGGGTGCTGTGTG	58.33%
Apta_940	DNA	TGTGCGGGGGAGGGGAGTGGCGCTGTTGGTGCGGGTATAGCGCGTGGTGT	56.86%
Apta_1257	DNA	CGACGCACAGTCGCTGGTGCGTACCTGGTTGCCGTTGTGT	56.86%
Apta_684	DNA	GGCGGACCAAGGGGACACCACAGATGAATGTACAGTACCATGTTACTGCGCCCGTAGGTG	56.67%
Apta_888	DNA	GGCGACCCCCGGGCTACCAGACAATGTACGCAGCAAGAGTGACGGTCGTACCTCGGAGTC	56.67%
Apta_417	DNA	GGCAGGAAGACAAACACCAGGTGAGGGAGACGACGCGAGTGTTAGATGGTAGCTGTTGGTCTGTGGTGCTGT	55.56%
Apta_902	DNA	ATACGGGAGCCAACACCACCAACATTATCAGAGTATGTTACTTATAGTGGTGGCAGAGCAGGTGTGACGGAT	55.56%
Apta_77	DNA	GGTGTGGGCGACATGGGGGTTGGTGGTGTAGGAGCGCCAT	54.90%
Apta_506	DNA	AAGCGCGCGAGACTACGTTTGTAGGGATACCGATGTCG	54.90%
Apta_1258	DNA	GGCGAAGGAGTCATGTAGGTGTGGTCGAGACCGCTGTGCT	54.90%
Apta_1259	DNA	GGCGCGGCATGGTGTGGACTCCAGGCGGTAGGGATGTCGT	54.90%
Apta_1261	DNA	GGGCGGACGCTAGGTGGTGATGCTGTGCTACACGTGTTGT	54.90%
Apta_988	DNA	AAGGATGACTGACGACGATGGGAATGGAGAGGAAGGGGATATAGGTATGTTGGATGTAG	54.24%
Apta_140	DNA	GGCAGGAAGACAAACACGATGGGGGGGTATGATTTGATGTGGTTGTTGCATGATCGTGGTCTGTGGTGCTGT	54.17%
Apta_901	DNA	ATACGGGAGCCAACACCACAAGTTGACTTGGAGGAGGGTCTAACTAGTTCGAGGAGAGCAGGTGTGACGGAT	54.17%
Apta_753	DNA	GGGCACAGACGGAAGATGAGAATTGTGGGGCTTAGTATAGTGAGGTGCGTGT	53.85%
Apta_1220	DNA	TGGCGACAAGAAGACGTACAAACACGCACCAGGCCGGAGTGGAGTATTCTGAGGTCGG	53.45%
Apta_794	DNA	ATCCAGAGTGACGCAGCAAGGAGATTCGAGGGGGAAGGTTTGTTATAGGGGTTAATGGACACGGTGGCTTAGT	53.42%
Apta_616	DNA	GGTGCGGGTAGGGATGAGGGCGGGTCGGGTTGAGTGTGAGCTTAGGGGtGGCGGATCTAC	53.33%

ABOUT