AptaDB: Result

Interaction ID: 481

Aptamer sequence: CCTATCTTATTCGTTGCGGACTCCCCGGTCGGTATGTATG

Target unique ID: Q61727

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_476	R5Cw1	Protein	I10R1_MOUSE	16 nM	150 mmol/L NaCl, 20 mmol/L KCl, and 2 mmol/L HEPES heated to 82 °C and slowly cooling to room temperature	22434135

Aptamer Sequence:	CCTATCTTATTCGTTGCGGACTCCCCGGTCGGTATGTATG
The optimal secondary structure in dot-bracket notation:	..............(((.((((....)))).)))......
The centroid secondary structure in dot-bracket notation:	...........((.(((.((((....)))).)))))....

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_476	Description	R5Cw1
Aptamer chemistry	DNA	Length	40 nt
GC content	52.5%	Molecular weight	12,235.83 Da
Molarity of 1 μg/μl solution	81.73 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted therapy
Sequence	CCTATCTTATTCGTTGCGGACTCCCCGGTCGGTATGTATG
Applications	Bound to IL-10 receptor on the cell surface and blocked IL-10 function in vitro

Type	Detail	Type	Detail
Uniprot ID	Q61727	Protein name	Interleukin-10 receptor subunit alpha (IL-10 receptor subunit alpha) (IL-10R subunit alpha) (IL-10RA) (CDw210a) (Interleukin-10 receptor subunit 1) (IL-10R subunit 1) (IL-10R1) (CD antigen CD210)
Gene name(s)	Il10ra Il10r	Organism	Interleukin-10 receptor subunit alpha (IL-10 receptor subunit alpha) (IL-10R subunit alpha) (IL-10RA) (CDw210a) (Interleukin-10 receptor subunit 1) (IL-10R subunit 1) (IL-10R1) (CD antigen CD210)
Status	reviewed in uniprot database	Length	575
Mass	64,248	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	N/A
Function	Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation.

No relevant experimental diagram	Interaction ID	481
	Target type	Protein
	Target unique ID	Q61727
	Activity	16 nM
	Binding Conditions/Buffer	150 mmol/L NaCl, 20 mmol/L KCl, and 2 mmol/L HEPES heated to 82 °C and slowly cooling to room temperature
	Assay	N/A
	PubMed ID	22434135

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_478	DNA	CTTCACTTTCGTGGACGGTGGCGTCGGGGCGGTTTGTATG	62.50%
Apta_474	DNA	CAAAAAGATAGGTGGATTTTTCCCCGGATCGGTTTGTATG	60.00%
Apta_475	DNA	CAAAAAGATAGGTGGATTTTTCCCCGGGTCGGTTTGTATG	60.00%
Apta_449	DNA	CGCACTATGTTTTACGAGCCGTTTCCTCGGCAGATAGTAAGTGCG	55.56%
Apta_820	DNA	TCCCACGATCTCATTAGTCTGTGGATAAGCGTGGGACGTCTATGA	55.56%
Apta_477	DNA	CGCGGAAGAATTGCTATGTGTGCCCCGGTTGGTCTGTATA	55.00%
Apta_577	DNA	ACTTCAGTGAGTTGTCCCACGGTCGGCGAGTCGGTGGTAG	55.00%
Apta_1036	DNA	ATACCAGTCTATTCAATTGGGCCCGTCCGTATGGTGGG	55.00%
Apta_1177	DNA	TCCTACTCGTGATATCGGTGTTTATGGTGTCTGTCTTCATGTAGGA	54.35%
Apta_65	DNA	CTACCGTGGTAGGGAAGGTTGGAGTGTAG	52.50%
Apta_150	DNA	CCGTAGGTTCGGGGCGGAGTGGTCCGGAAGGTGGCGTGG	52.50%
Apta_162	DNA	CCGTACATACTACGTACGTACGTGAACAGAGGCAGAG	52.50%
Apta_604	DNA	TCCTGACCTTAGCCTATGTTTTCTCTACCGGCTATGTTAC	52.50%
Apta_671	DNA	GCATCTGATCGGGTGTGGGTGGCGTAAAGG	52.50%
Apta_683	DNA	GCAGTACGATCGGGGGTGGGTGGATGTAGG	52.50%
Apta_693	DNA	CGAGTTGAGCCGGGCGCGGTACGGGTACTGGTATGTGTGG	52.50%
Apta_1061	DNA	TATTTATTGAATGCTGTAATTTCGCCACGCGAAAGTAGG	52.50%
Apta_1149	DNA	ATCATGGTGGGTATCGGCACTCGTTGGTTGAT	52.50%
Apta_1263	DNA	CCCCGCTGCCCGTGTTCCGTCCTCCCTTGCTGTGTGTGCG	52.50%
Apta_1081	DNA	CTTACGACTGTGGTCGGGTGGTGGGCCTCTAGAGGGGTGTCGTAAG	52.17%

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