Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 745
Aptamer sequence: GGCGGACCAAGGGGACACCACAGATGAATGTACAGTACCATGTTACTGCGCCCGTAGGTG
Target unique ID: 446512
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_684 | OA 24 | Molecule | Okadaic acid | 126 nM | 50 mM Tris, pH 7.5, 150 mM NaCl, 2 mM MgCl2 | 24164310 |
Structure information of aptamer
| Aptamer Sequence: | GGCGGACCAAGGGGACACCACAGATGAATGTACAGTACCATGTTACTGCGCCCGTAGGTG |
| The optimal secondary structure in dot-bracket notation: | ((((.......((....))(((......))).(((((......)))))))))........ |
| The centroid secondary structure in dot-bracket notation: | ((((...............(((......))).(((((......)))))))))........ |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_684 | Description | OA 24 |
| Aptamer chemistry | DNA | Length | 60 nt |
| GC content | 56.7% | Molecular weight | 18,583.92 Da |
| Molarity of 1 μg/μl solution | 53.81 μM | Number of G-quadruplexes | 2 |
| G-Score | 17 | Function | Aptasensor |
| Sequence | GGCGGACCAAGGGGACACCACAGATGAATGTACAGTACCATGTTACTGCGCCCGTAGGTG | ||
| Applications | Detection of okadaic acid (OA) | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| PubChem ID | 446512 | Molecular name | Okadaic acid |
| Molecular formula | C44H68O13 | Molecular weight | 805 |
| IUPAC name | (2R)-3-[(2S,6R,8S,11R)-2-[(E,2R)-4-[(2S,2R,4R,4aS,6R,8aR)-4-hydroxy-2-[(1S,3S)-1-hydroxy-3-[(2S,3R,6S)-3-methyl-1,7-dioxaspiro[5.5]undecan-2-yl]butyl]-3-methylidenespiro[4a,7,8,8a-tetrahydro-4H-pyrano[3,2-b]pyran-6,5-oxolane]-2-yl]but-3-en-2-yl]-11-hydroxy-4-methyl-1,7-dioxaspiro[5.5]undec-4-en-8-yl]-2-hydroxy-2-methylpropanoic acid | InChIKey | QNDVLZJODHBUFM-WFXQOWMNSA-N |
| LogP | 5.214 | Topological polar surface area | 182.83 |
| Hydrogen bond acceptor | 12 | Hydrogen bond donor | 5 |
Synonym(s): okadaic acid; 78111-17-8; C44H68O13; 9,10-Deepithio-9,10-didehydroacanthifolicin; CHEMBL280487; CHEBI:44658; MFCD00083455; UNII-1W21G5Q4N2; (2R)-3-[(2S,5R,6R,8S)-8-[(2R,3E)-4-[(2R,4'aR,5R,6'S,8'R,8'aS)-8'-hydroxy-6'-[(1S,3S)-1-hydroxy-3-[(2S,3R,6S)-3-methyl-1,7-dioxaspiro[5.5]undecan-2-yl]butyl]-7'-methylidene-hexahydro-3'H-spiro[oxolane-2,2'-pyrano[3,2-b]pyran]-5-yl]but-3-en-2-yl]-5-hydroxy-10-methyl-1,7-dioxaspiro[5.5]undec-10-en-2-yl]-2-hydroxy-2-methylpropanoic acid; OKA; 1W21G5Q4N2; Okadaic acid (+Na); Okadaic acid from Prorocentrum concavum; 1jk7; Probes1_000180; SCHEMBL131485; CHEMBL158524; GTPL5349; DTXSID60880002; 1u32; HY-N6785; BDBM50097634; BDBM50110676; ZINC85601542; DB02169; CS-0014219; Q414042; Okadaic acid from Prorocentrum concavum, 92-100% (HPLC); Okadaic acid from Prorocentrum concavum, >=90% (HPLC), translucent film; (2R)-2-hydroxy-3-[(2S,5R,6R,8S)-5-hydroxy-8-{(1R,2E)-3-[(2R,4a'R,5R,6'S,8'R,8a'S)-8'-hydroxy-6'-{(1S,3S)-1-hydroxy-3-[(2S,3R,6S)-3-methyl-1,7-dioxaspiro[5.5]undec-2-yl]butyl}-7'-methylideneoctahydro-3H,3'H-spiro[furan-2,2'-pyrano[3,2-b]pyran]-5-yl]-1-methylprop-2-en-1-yl}-10-methyl-1,7-dioxaspiro[5.5]undec-10-en-2-yl]-2-methylpropanoic acid
Activity data
|
No relevant experimental diagram |
Interaction ID | 745 |
| Target type | Molecule | |
| Target unique ID | 446512 | |
| Activity | 126 nM | |
| Binding Conditions/Buffer | 50 mM Tris, pH 7.5, 150 mM NaCl, 2 mM MgCl2 |
|
| Assay | N/A |
|
| PubMed ID | 24164310 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_888 | DNA | GGCGACCCCCGGGCTACCAGACAATGTACGCAGCAAGAGTGACGGTCGTACCTCGGAGTC | |
| Apta_662 | DNA | GGCGCCAAACAGGACCACCATGACAATTACCCATACCACCTCATTATGCCCCATCTCCGC | |
| Apta_755 | DNA | GGGGAGGGAGACACAGTCATGGAGCAGTTATTAGGGTGTACCGGGTGTAGT | |
| Apta_104 | DNA | AGCAGCACAGAGGTCAGATGCAAACCACAGCCTGAGTGGTTAGCGTATGTCATTTACGGACCTATGCGTGCTACCGTGAA | |
| Apta_104 | DNA | AGCAGCACAGAGGTCAGATGCAAACCACAGCCTGAGTGGTTAGCGTATGTCATTTACGGACCTATGCGTGCTACCGTGAA | |
| Apta_585 | DNA | GATCGAGGGCAGCGATAGCTGGGCTAATAAGGTTAGCCCCATCGGTC | |
| Apta_685 | DNA | GGGGCAACAAACACGGAAGAAAATGAATCTACATACGTGGACATATATCCTGCCGCGTG | |
| Apta_728 | DNA | CGACCCGGCACAAACCCAGAACCATATACACGATCATTAGTCTCCTGGGCCG | |
| Apta_1068 | DNA | CGGGACAAGACAACGAACGAACAGGAAGAGAACCGGAATGCAGACGTCAGGG | |
| Apta_1267 | DNA | GGCACGGCAAAGGGGTACAGCCTACCGAACCGTGGCTGTAAGGGTGGTTGTGGTGTG | |
| Apta_790 | DNA | GGGAGACAAGAATAAACGCTCAAGAAGTGAAAATGACAGAACACAACATTCGACAGGAGGCTCACAACAGGC | |
| Apta_1178 | DNA | AGCGTCGAATACCACTACAGGGGATGCGAAGTTCCGCGGTCGAGTATATGATACATCCATCTAATGGAGCTCGTGGTCAG | |
| Apta_639 | DNA | CTAAGCACAGGGAAACCAGCTAATGAGTTAGGCCTGTCCCCCACG | |
| Apta_663 | DNA | CACGCACAGAAGACACCTACAGGGCCAGATCACAATCGGTTAGTGAACTCGTACGGCGCG | |
| Apta_819 | DNA | GCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTAT | |
| Apta_983 | DNA | ATGGGAACAGAGCGGCGCAGAGACCCAATGCCACGAGTACCTGACCCTATCAACCCGTGA | |
| Apta_991 | DNA | AGCAGTGAAGAGGCGAGAGATTTATGAGGGTAGCGTCCACATATGCCAGGATCGTGTTG | |
| Apta_109 | DNA | AGCAGCACAGAGGTCAGATGTGCGACCACCTGGATAGACGAGGGTAGTAAGGAGTGTGTTCCTATGCGTGCTACCGTGA | |
| Apta_109 | DNA | AGCAGCACAGAGGTCAGATGTGCGACCACCTGGATAGACGAGGGTAGTAAGGAGTGTGTTCCTATGCGTGCTACCGTGA | |
| Apta_1105 | DNA | TACGACTCACTATAGGGATCCAATGGCACAGCGCCTGGAACGTACTCTGTACCTGGAATTCCCTTTAGTGAGGGTT |
DEV TOOL
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