Aptamer-target interaction descriptor
Aptamer-target interaction information
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_1194 | HGFap2 | Protein | HGF_HUMAN | 45 nM | 10 mM Tris-HCl, 150 mM NaCl, and 100 mM KCl | 28627727 |
Structure information of aptamer
| Aptamer Sequence: | GGGAGAGGTGGGAGCGGGGCCAGGG |
| The optimal secondary structure in dot-bracket notation: | ........(((........)))... |
| The centroid secondary structure in dot-bracket notation: | ......................... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_1194 | Description | HGFap2 |
| Aptamer chemistry | DNA | Length | 25 nt |
| GC content | 80.0% | Molecular weight | 7,959.10 Da |
| Molarity of 1 μg/μl solution | 125.64 μM | Number of G-quadruplexes | 3 |
| G-Score | 62 | Function | Target detection |
| Sequence | GGGAGAGGTGGGAGCGGGGCCAGGG | ||
| Applications | Detect hepatocyte growth factor | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P14210 | Protein name | Hepatocyte growth factor (Hepatopoietin-A) (Scatter factor) (SF) [Cleaved into: Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain] |
| Gene name(s) | HGF HPTA | Organism | Hepatocyte growth factor (Hepatopoietin-A) (Scatter factor) (SF) [Cleaved into: Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain] |
| Status | reviewed in uniprot database | Length | 728 |
| Mass | 83,134 | Activity regulation | N/A |
| Involvement in disease | Deafness, autosomal recessive, 39 (DFNB39) | Catalytic activity | N/A |
| PDB ID(s) | 1BHT;1GMN;1GMO;1GP9;1NK1;1SHY;1SI5;2HGF;2QJ2;3HMS;3HMT;3HN4;3MKP;3SP8;4D3C;4K3J;4O3T;4O3U;5COE;5CP9;5CS1;5CS3;5CS5;5CS9;5CSQ;5CT1;5CT2;5CT3;7MO7;7MO8;7MO9;7MOA;7MOB;7OCL;7OCM; |
 |
Function | Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. |
Activity data
 |
Interaction ID | 1256 |
| Target type | Protein | |
| Target unique ID | P14210 | |
| Activity | 45 nM | |
| Binding Conditions/Buffer | 10 mM Tris-HCl, 150 mM NaCl, and 100 mM KCl |
|
| Assay | SPR sensorgram of PQSs bound to HGF or HBEGF protein |
|
| PubMed ID | 28627727 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_10 | DNA | GGGGGGGGTGGCTGGGCGGGGTGGG | |
| Apta_1195 | DNA | GGGGTTGGGGGGAGGCGGGGAATGGGGG | |
| Apta_27 | DNA | AGGGGGAGGGGTGGGGCCGGGCAGA | |
| Apta_1193 | DNA | GGGTTGGAGGTGGAGGGGAGTTGAGG | |
| Apta_71 | DNA | GGCGAGTAGGTTGGTGTGGTTGGGGCTCGCC | |
| Apta_46 | DNA | CGGGTGGGAGTTGTGGGGGGGGGTGGGAGGGTT | |
| Apta_9 | DNA | TGAGGGGGCGGCGGGGCCGGGCAGG | |
| Apta_13 | DNA | AGGGGGGGGGGAGGGGTCGGGGTGG | |
| Apta_30 | DNA | AGAGGGGGGGGTGGAGGGGGCTAGA | |
| Apta_60 | DNA | GGAATGAGTGCCCTGCAAGCGAGGGCTAGC | |
| Apta_678 | DNA | CGTACGGTGGGAACGGTTCCTCTTAGGGT | |
| Apta_706 | DNA | CCGGCCAAGGGTGGGAGGGAGGGGGCCGG | |
| Apta_69 | DNA | AGCGAGTAGGTTGGTGTGGTTGGGGCTCGCT | |
| Apta_73 | DNA | GGGGAGTAGGTTGGTGTGGTTGGGGCTCCCC | |
| Apta_75 | DNA | CACTGGTAGGTTGGTGTGGTTGGGGCCAGTG | |
| Apta_350 | DNA | GGTGGTGGTGGTTGTGGTGGTGGTGG | |
| Apta_1261 | DNA | GGGCGGACGCTAGGTGGTGATGCTGTGCTACACGTGTTGT | |
| Apta_245 | DNA | ACAGGGGTGTGGGGACAGGGGTGTGGGG | |
| Apta_1210 | DNA | GGGGAGGCAGTGTGTTGTGTCGTGTGTGTGCTTGG | |
| Apta_679 | DNA | CAGGTGGCAGATCGGGTGTGGGTGGCCTGG |
DEV TOOL
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